ZKSCAN5 (NM_145102) Human Tagged ORF Clone

CAT#: RG215697

ZKSCAN5 (tGFP-tagged) - Human zinc finger with KRAB and SCAN domains 5 (ZKSCAN5), transcript variant 2

Product Images

Circular map for RG215697

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZKSCAN5
• Synonyms: ZFP-95; ZFP95; ZNF914; ZSCAN37
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215697 representing NM_145102
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATAATGACCGAATCCCGAGAAGTTATAGACTTAGACCCCCCAGCTGAGACTTCCCAGGAGCAGGAAG
  ACCTTTTCATAGTGAAGGTGGAAGAAGAAGACTGCACCTGGATGCAGGAGTACAACCCGCCAACGTTTGA
  GACTTTTTACCAGCGCTTCAGGCACTTCCAGTACCATGAGGCTTCAGGACCCCGGGAGGCTCTCAGCCAA
  CTCCGGGTGCTCTGCTGTGAGTGGCTGAGGCCCGAGCTGCACACGAAGGAGCAGATCCTGGAGCTGCTGG
  TGCTGGAGCAGTTCCTGACCATCCTGCCTGAAGAGTTCCAGCCCTGGGTGAGGGAACATCACCCTGAAAG
  TGGAGAAGAGGCGGTGGCCGTGATAGAAAATATACAGCGAGAACTTGAGGAACGCAGACAGCAGATTGTT
  GCCTGCCCTGATGTGCTTCCTCGGAAGATGGCAACACCTGGAGCAGTGCAGGAGTCCTGCAGCCCCCATC
  CCCTGACCGTGGACACCCAGCCTGAGCAAGCGCCACAGAAGCCTCGTCTCCTGGAGGAAAATGCCCTTCC
  TGTTCTCCAAGTTCCTTCCCTTCCCCTGAAGGACAGCCAGGAGCTGACAGCTTCACTTCTCTCAACTGGG
  TCCCAGAAGTTGGTGAAAATTGAAGAGGTGGCTGATGTGGCTGTATCCTTCATCCTGGAGGAATGGGGGC
  ATTTGGACCAGTCCCAGAAGTCCCTTTATAGGGATGACAGGAAGGAGAACTATGGGAGTATTACTTCCAT
  GGGTTATGAGTCCAGGGACAATATGGAGCTCATAGTGAAGCAGATTTCTGATGACTCTGAATCACACTGG
  GTGGCGCCAGAACACACCGAAAGGAGCGTTCCTCAGGATCCAGACTTTGCAGAAGTCAGTGACCTTAAAG
  GCATGGTACAAAGGTGGCAGGTCAACCCCACTGTGGGGAAATCAAGGCAGAATCCTTCCCAGAAAAGGGA
  TCTGGATGCAATCACAGACATCAGCCCTAAGCAAAGCACACATGGCGAGAGAGGGCACAGATGCAGCGAT
  TGTGGCAAATTCTTCCTCCAAGCCTCAAACTTTATTCAGCATCGGCGCATCCACACTGGAGAAAAACCGT
  TTAAGTGCGGAGAATGTGGGAAGAGCTACAATCAGCGGGTGCACCTCACCCAGCACCAGCGCGTCCACAC
  AGGGGAGAAACCCTACAAATGTCAGGTGTGCGGAAAGGCTTTCCGGGTGAGTTCCCACCTGGTTCAGCAC
  CACAGTGTCCACAGCGGAGAGAGGCCCTATGGCTGCAATGAGTGTGGGAAGAACTTCGGTCGCCATTCGC
  ATCTGATCGAACACCTAAAACGCCACTTCAGGGAGAAATCCCAGAGATGCAGTGACAAAAGAAGTAAGAA
  CACAAAATTAAGTGTTAAGAAGAAAATTTCAGAATATTCAGAAGCAGACATGGAACTATCTGGAAAAACC
  CAAAGAAATGTTTCTCAAGTTCAAGATTTTGGAGAAGGCTGTGAGTTTCAAGGCAAGCTGGATAGAAAGC
  AGGGAATTCCCATGAAAGAGATACTAGGACAACCATCTTCAAAGAGGATGAACTACAGTGAAGTCCCATA
  TGTCCACAAAAAATCCTCCACTGGAGAGAGACCACATAAATGTAACGAGTGTGGGAAAAGCTTCATTCAG
  AGTGCACATCTTATTCAACATCAAAGAATACACACTGGGGAGAAACCATTCAGGTGTGAGGAATGTGGGA
  AAAGCTACAACCAACGCGTGCACCTAACTCAGCATCAGCGCGTCCACACAGGTGAGAAGCCCTACACCTG
  TCCCTTATGTGGGAAAGCCTTCAGAGTGAGGTCCCACCTTGTTCAGCATCAGAGCGTGCACAGTGGGGAG
  AGACCCTTCAAGTGTAACGAATGTGGGAAAGGCTTTGGGAGGCGTTCCCACCTGGCTGGACATCTTCGAC
  TCCACTCCCGAGAGAAATCCCATCAGTGTCGTGAATGTGGGGAAATCTTTTTTCAGTACGTTAGCCTAAT
  TGAACATCAGGTGCTCCACATGGGTCAGAAAAATGAAAAAAATGGCATCTGTGAGGAAGCATATAGTTGG
  AACTTGACAGTGATTGAAGACAAGAAGATTGAGTTACAAGAGCAGCCTTATCAGTGTGATATCTGTGGAA
  AAGCCTTTGGTTATAGCTCAGACCTCATTCAGCATTACAGAACTCATACAGCAGAGAAGCCCTATCAATG
  TGATATATGTAGAGAAAATGTTGGCCAGTGTTCCCACACCAAACAACATCAAAAAATCTACTCCAGCACA
  AAATCCCATCAATGTCATGAATGTGGCAGAGGCTTCACTCTGAAGTCACATCTTAATCAACATCAGAGAA
  TCCATACTGGTGAGAAACCTTTTCAATGTAAAGAATGTGGAATGAATTTCAGCTGGAGTTGTAGCCTCTT
  TAAACACCTGAGAAGCCATGAGAGGACAGATCCCATAAATACCTTAAGTGTAGAGGGGTCTCTGTTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215697 representing NM_145102
  Red=Cloning site Green=Tags(s)
  
  MIMTESREVIDLDPPAETSQEQEDLFIVKVEEEDCTWMQEYNPPTFETFYQRFRHFQYHEASGPREALSQ
  LRVLCCEWLRPELHTKEQILELLVLEQFLTILPEEFQPWVREHHPESGEEAVAVIENIQRELEERRQQIV
  ACPDVLPRKMATPGAVQESCSPHPLTVDTQPEQAPQKPRLLEENALPVLQVPSLPLKDSQELTASLLSTG
  SQKLVKIEEVADVAVSFILEEWGHLDQSQKSLYRDDRKENYGSITSMGYESRDNMELIVKQISDDSESHW
  VAPEHTERSVPQDPDFAEVSDLKGMVQRWQVNPTVGKSRQNPSQKRDLDAITDISPKQSTHGERGHRCSD
  CGKFFLQASNFIQHRRIHTGEKPFKCGECGKSYNQRVHLTQHQRVHTGEKPYKCQVCGKAFRVSSHLVQH
  HSVHSGERPYGCNECGKNFGRHSHLIEHLKRHFREKSQRCSDKRSKNTKLSVKKKISEYSEADMELSGKT
  QRNVSQVQDFGEGCEFQGKLDRKQGIPMKEILGQPSSKRMNYSEVPYVHKKSSTGERPHKCNECGKSFIQ
  SAHLIQHQRIHTGEKPFRCEECGKSYNQRVHLTQHQRVHTGEKPYTCPLCGKAFRVRSHLVQHQSVHSGE
  RPFKCNECGKGFGRRSHLAGHLRLHSREKSHQCRECGEIFFQYVSLIEHQVLHMGQKNEKNGICEEAYSW
  NLTVIEDKKIELQEQPYQCDICGKAFGYSSDLIQHYRTHTAEKPYQCDICRENVGQCSHTKQHQKIYSST
  KSHQCHECGRGFTLKSHLNQHQRIHTGEKPFQCKECGMNFSWSCSLFKHLRSHERTDPINTLSVEGSLL
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145102
• ORF Size: 2517 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145102.4
• RefSeq Size: 4193 bp
• RefSeq ORF: 2520 bp
• Locus ID: 23660
• UniProt ID: Q9Y2L8
• Cytogenetics: 7q22.1
• Domains: KRAB, LER, zf-C2H2
• Protein Families: Transcription Factors
• Gene Summary: This gene encodes a zinc finger protein of the Kruppel family. The protein contains a SCAN box and a KRAB A domain and may be involved in transcriptional regulation. A similar protein in mouse is differentially expressed in spermatogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]