RUSC2 (NM_014806) Human Tagged ORF Clone

CAT#: RG215610

RUSC2 (tGFP-tagged) - Human RUN and SH3 domain containing 2 (RUSC2)

Product Images

Circular map for RG215610

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RUSC2
• Synonyms: Iporin; MRT61
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215610 representing NM_014806
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATAGTCCCCCAAAGCTGACTGGAGAGACCCTCATCGTTCATCACATCCCCCTGGTGCACTGCCAAG
  TCCCAGACAGGCAGTGCTGTGGAGGGGCAGGTGGAGGTGGTGGGAGCACAAGACCTAATCCCTTCTGCCC
  ACCTGAGCTGGGCATCACCCAGCCCGATCAAGACCTAGGACAAGCTGACTCCCTGCTATTCAGCAGCCTG
  CACTCTGCTCCAGGAGGAACTGCACGGTCTATAGACAGCACCAAGAGTAGGAGTCGGGATGGAAGAGGCC
  CTGGAGCCCCCAAACGACACAACCCCTTCTTGCTGCAGGAGGGTGTGGGTGAGCCAGGACTTGGTGACCT
  GTATGATGACAGCATTGGTGACAGTGCCACCCAGCAGTCCTTCCACCTGCATGGCACTGGCCAGCCCAAC
  TTTCATCTATCCTCTTTCCAGCTGCCACCATCTGGCCCCAGAGTGGGCAGGCCATGGGGGACAACACGCA
  GTCGGGCTGGAGTGGTGGAAGGGCAGGAACAGGAGCCAGTGATGACCTTGGATACTCAGCAGTGCGGCAC
  CAGCCACTGCTGCCGGCCAGAGCTGGAAGCAGAGACTATGGAGCTGGATGAGTGTGGGGGACCTGGTGGG
  AGTGGCAGTGGGGGTGGAGCCAGCGATACCTCTGGCTTTTCCTTTGACCAGGAATGGAAGCTCAGTTCAG
  ATGAATCCCCAAGGAACCCTGGATGCTCCGGCTCAGGGGACCAGCACTGCCGCTGCAGTAGCACATCCAG
  TCAGTCCGAGGCAGCTGACCAGTCCATGGGCTATGTGAGCGACTCCTCCTGCAACAGTTCAGATGGTGTG
  CTGGTCACCTTCAGCACCCTCTACAACAAGATGCATGGCACCCCCCGTGCCAATCTCAACTCTGCCCCAC
  AGTCCTGCAGCGACTCTTCCTTCTGCAGCCACTCAGACCCTGGCGCCTTCTATCTGGATCTGCAGCCCTC
  CCCATTTGAGTCTAAGATGTCTTATGAGTCCCATCACCCTGAAAGTGGAGGAAGGGAAGGGGGCTATGGT
  TGCCCTCATGCCTCTTCTCCTGAGCTTGATGCCAACTGCAACTCCTACCGCCCACACTGTGAGCCGTGCC
  CAGCAGTGGCTGACCTCACAGCCTGCTTCCAAAGCCAGGCCCGTCTTGTTGTGGCCACACAAAATTACTA
  TAAACTTGTCACCTGTGACCTATCTTCCCAATCATCCCCAAGCCCTGCTGGCTCTTCCATCACTAGCTGC
  TCTGAGGAACACACCAAGATAAGTCCCCCACCAGGCCCTGGCCCAGACCCAGGCCCCAGCCAGCCCTCTG
  AGTATTACCTATTCCAGAAGCCAGAAGTCCAGCCAGAGGAACAAGAAGCAGTGAGTTCCTCCACCCAAGC
  AGCAGCTGCTGTGGGCCCCACTGTGCTTGAGGGACAAGTATACACGAATACTTCACCCCCCAACCTCAGC
  ACTGGACGTCAGCGCTCCCGCAGCTATGATCGCAGCCTGCAGCGCAGCCCTCCTGTCCGCCTGGGCTCGC
  TGGAACGTATGTTGAGTTGCCCAGTGCGCTTGAGTGAGGGCCCTGCAGCCATGGCCGGGCCTGGCTCCCC
  ACCCAGGAGGGTCACCTCCTTTGCCGAGCTGGCCAAGGGCCGGAAGAAAACTGGAGGCTCTGGCTCGCCC
  CCACTTCGTGTGAGTGTTGGGGACTCCTCCCAGGAGTTCTCACCCATCCAAGAAGCCCAGCAAGATCGGG
  GGGCCCCACTGGATGAGGGCACTTGCTGTAGCCATAGCCTGCCACCCATGCCTTTGGGGCCAGGCATGGA
  CCTACTTGGCCCAGACCCAAGTCCACCCTGGTCCACCCAGGTCTGTCAGGGACCCCACTCCAGTGAGATG
  CCTCCTGCTGGCCTCAGAGCTACTGGGCAAGGCCCCCTGGCTCAGCTGATGGATCCAGGGCCTGCTCTCC
  CAGGGAGCCCAGCCAACAGCCATACCCAGAGGGATGCAAGAGCTAGAGCTGACGGGGGTGGCACCGAGAG
  CCGACCAGTCCTTCGCTACAGCAAGGAACAGAGGCCAACCACACTGCCCATCCAGCCCTTCGTGTTCCAG
  CACCACTTCCCCAAGCAGCTGGCCAAGGCCCGGGCCCTCCACAGCCTTTCCCAGCTCTACAGCCTCTCAG
  GCTGCAGCCGTACACAGCAGCCTGCCCCACTGGCTGCCCCTGCTGCTCAAGTCTCAGTCCCAGCTCCCTC
  AGGGGAACCGCAGGCATCCACTCCCCGAGCCACTGGCAGAGGTGCCAGGAAAGCTGGGTCTGAGCCAGAG
  ACCTCTCGGCCATCGCCCCTGGGCAGCTACTCCCCCATCCGGAGTGTTGGCCCCTTTGGGCCCAGCACTG
  ACTCTTCTGCCTCCACTTCGTGCTCCCCTCCCCCAGAGCAGCCCACAGCCACAGAAAGCCTGCCCCCATG
  GAGCCACTCCTGTCCTTCTGCTGTCCGGCCTGCCACCTCCCAGCAGCCGCAGAAGGAGGATCAGAAGATA
  CTGACCTTGACTGAGTACCGGCTCCATGGAACAGGAAGCTTGCCGCCTCTGGGCTCCTGGCGATCTGGCC
  TCAGCCGAGCAGAGAGCCTGGCCCGGGGAGGTGGTGAGGGCAGCATGGCCACCAGGCCCAGTAATGCCAA
  CCACCTATCCCCTCAAGCCCTCAAGTGGCGGGAATACAGGAGGAAGAACCCACTAGGGCCACCTGGTTTG
  TCAGGGAGCCTAGACCGAAGATCACAAGAAGCTCGGCTGGCCCGAAGAAACCCTATCTTTGAGTTCCCTG
  GCTCCCTCAGTGCTGCCAGCCATCTGAACTGCCGGCTGAATGGCCAAGCAGTGAAGCCGTTACCACTGAC
  CTGCCCTGACTTCCAGGACCCCTTTTCCTTGACGGAGAAGCCTCCAGCTGAGTTTTGTCTGTCCCCAGAT
  GGCAGCTCAGAGGCCATTTCCATTGACCTGCTTCAGAAAAAAGGGCTGGTAAAAGCTGTTAACATCGCTG
  TGGACCTCATTGTGGCTCATTTTGGCACAAGCCGGGATCCCGGGGTGAAGGCAAAGCTGGGAAACAGTTC
  TGTGAGCCCCAATGTGGGCCACCTGGTTCTGAAGTACTTGTGCCCTGCCGTCCGCGCCGTGCTGGAGGAT
  GGGCTCAAGGCCTTTGTACTGGACGTCATCATCGGGCAGCGTAAGAACATGCCATGGAGTGTGGTTGAGG
  CTTCCACACAGCTAGGCCCATCCACCAAGGTCCTGCATGGCCTCTACAACAAAGTCAGCCAATTCCCAGA
  GCTCACCAGTCATACCATGCGCTTCAACGCCTTCATCCTCGGCCTGCTCAACATCCGGTCCCTGGAGTTC
  TGGTTTAATCACCTCTATAACCACGAAGACATCATCCAGACCCACTACCAGCCCTGGGGCTTCCTGAGTG
  CAGCTCATACCGTGTGTCCCGGCCTCTTTGAAGAGCTGCTGCTGCTGCTACAGCCCCTGGCCCTGCTGCC
  CTTCAGCCTCGACTTGCTGTTCCAGCACCGGCTGCTGCAAAGTGGGCAGCAGCAGCGGCAGCACAAGGAA
  CTGCTGCGGGTGTCCCAGGACCTGCTGCTGTCTGCCCACTCCACGCTGCAGCTGGCCCGGGCCCGGGGCC
  AGGAGGGCCCTGGAGACGTGGACAGGGCAGCCCAAGGGGAGCGGGTGAAGGGTGTGGGTGCCTCAGAAGG
  TGGAGAAGAGGAAGAGGAAGAAGAGGAGACAGAAGAGGTGGCAGAGGCAGCCGGGGGCTCAGGGCGTGCC
  AGGTGGGCCCGAGGTGGGCAGGCCGGCTGGTGGTACCAGCTCATGCAGAGCTCCCAGGTCTACATCGATG
  GCTCCATTGAGGGTTCCAGGTTCCCTCGTGGTAGCAGCAACAGCAGCAGCGAGAAAAAGAAAGGGGCAGG
  AGGTGGGGGACCTCCCCAGGCTCCACCACCCCGAGAGGGAGTAGTGGAGGGGGCTGAGGCCTGCCCTGCC
  TCTGAGGAGGCCCTGGGCCGGGAAAGGGGCTGGCCCTTCTGGATGGGGAGCCCCCCTGACTCTGTGCTGG
  CCGAGCTGAGGCGCAGTCGGGAGAGGGAAGGGCCCGCTGCCTCGCCAGCAGAAAATGAGGAAGGGGCCTC
  AGAGCCTTCACCTGGAGGCATCAAGTGGGGACACCTCTTTGGCTCCCGAAAAGCCCAGCGGGAGGCCCGG
  CCCACAAATAGGCTCCCCTCGGACTGGCTGAGCCTGGACAAGTCCATGTTCCAACTAGTGGCGCAGACAG
  TGGGTTCCCGCCGGGAGCCAGAGCCCAAGGAGAGCCTGCAGGAGCCACACTCCCCAGCCCTGCCCTCCAG
  TCCTCCGTGTGAGGTGCAGGCACTGTGCCACCACCTGGCCACCGGCCCTGGACAGCTGAGCTTCCACAAA
  GGAGACATCCTACGAGTGCTGGGGCGAGCTGGAGGAGACTGGCTGCGCTGCAGCCGTGGCCCCGACTCTG
  GCCTGGTGCCCCTGGCCTACGTGACATTGACCCCAACTCCAAGTCCAACCCCTGGAAGCAGCCAAAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215610 representing NM_014806
  Red=Cloning site Green=Tags(s)
  
  MDSPPKLTGETLIVHHIPLVHCQVPDRQCCGGAGGGGGSTRPNPFCPPELGITQPDQDLGQADSLLFSSL
  HSAPGGTARSIDSTKSRSRDGRGPGAPKRHNPFLLQEGVGEPGLGDLYDDSIGDSATQQSFHLHGTGQPN
  FHLSSFQLPPSGPRVGRPWGTTRSRAGVVEGQEQEPVMTLDTQQCGTSHCCRPELEAETMELDECGGPGG
  SGSGGGASDTSGFSFDQEWKLSSDESPRNPGCSGSGDQHCRCSSTSSQSEAADQSMGYVSDSSCNSSDGV
  LVTFSTLYNKMHGTPRANLNSAPQSCSDSSFCSHSDPGAFYLDLQPSPFESKMSYESHHPESGGREGGYG
  CPHASSPELDANCNSYRPHCEPCPAVADLTACFQSQARLVVATQNYYKLVTCDLSSQSSPSPAGSSITSC
  SEEHTKISPPPGPGPDPGPSQPSEYYLFQKPEVQPEEQEAVSSSTQAAAAVGPTVLEGQVYTNTSPPNLS
  TGRQRSRSYDRSLQRSPPVRLGSLERMLSCPVRLSEGPAAMAGPGSPPRRVTSFAELAKGRKKTGGSGSP
  PLRVSVGDSSQEFSPIQEAQQDRGAPLDEGTCCSHSLPPMPLGPGMDLLGPDPSPPWSTQVCQGPHSSEM
  PPAGLRATGQGPLAQLMDPGPALPGSPANSHTQRDARARADGGGTESRPVLRYSKEQRPTTLPIQPFVFQ
  HHFPKQLAKARALHSLSQLYSLSGCSRTQQPAPLAAPAAQVSVPAPSGEPQASTPRATGRGARKAGSEPE
  TSRPSPLGSYSPIRSVGPFGPSTDSSASTSCSPPPEQPTATESLPPWSHSCPSAVRPATSQQPQKEDQKI
  LTLTEYRLHGTGSLPPLGSWRSGLSRAESLARGGGEGSMATRPSNANHLSPQALKWREYRRKNPLGPPGL
  SGSLDRRSQEARLARRNPIFEFPGSLSAASHLNCRLNGQAVKPLPLTCPDFQDPFSLTEKPPAEFCLSPD
  GSSEAISIDLLQKKGLVKAVNIAVDLIVAHFGTSRDPGVKAKLGNSSVSPNVGHLVLKYLCPAVRAVLED
  GLKAFVLDVIIGQRKNMPWSVVEASTQLGPSTKVLHGLYNKVSQFPELTSHTMRFNAFILGLLNIRSLEF
  WFNHLYNHEDIIQTHYQPWGFLSAAHTVCPGLFEELLLLLQPLALLPFSLDLLFQHRLLQSGQQQRQHKE
  LLRVSQDLLLSAHSTLQLARARGQEGPGDVDRAAQGERVKGVGASEGGEEEEEEEETEEVAEAAGGSGRA
  RWARGGQAGWWYQLMQSSQVYIDGSIEGSRFPRGSSNSSSEKKKGAGGGGPPQAPPPREGVVEGAEACPA
  SEEALGRERGWPFWMGSPPDSVLAELRRSREREGPAASPAENEEGASEPSPGGIKWGHLFGSRKAQREAR
  PTNRLPSDWLSLDKSMFQLVAQTVGSRREPEPKESLQEPHSPALPSSPPCEVQALCHHLATGPGQLSFHK
  GDILRVLGRAGGDWLRCSRGPDSGLVPLAYVTLTPTPSPTPGSSQN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014806
• ORF Size: 4548 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014806.4
• RefSeq Size: 5199 bp
• RefSeq ORF: 4551 bp
• Locus ID: 9853
• UniProt ID: Q8N2Y8
• Cytogenetics: 9p13.3
• Gene Summary: This gene encodes a RUN and SH3 domain containing protein that interacts with Rab1b and Rab1-binding protein GM130. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jun 2012]