GRIN3A (NM_133445) Human Tagged ORF Clone

CAT#: RG215549

GRIN3A (tGFP-tagged) - Human glutamate receptor, ionotropic, N-methyl-D-aspartate 3A (GRIN3A)

Product Images

Circular map for RG215549

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GRIN3A
• Synonyms: GluN3A; NMDAR-L; NMDAR3A; NR3A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215549 representing NM_133445
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAGACTGAGTTTGTGGTGGCTGCTGAGCAGGGTCTGTCTGCTGTTGCCGCCGCCCTGCGCACTGG
  TGCTGGCCGGGGTGCCCAGCTCCTCCTCGCACCCGCAGCCCTGCCAGATCCTCAAGCGCATCGGGCACGC
  GGTGAGGGTGGGCGCGGTGCACTTGCAGCCCTGGACCACCGCCCCCCGCGCGGCCAGCCGCGCTCCGGAC
  GACAGCCGAGCAGGAGCCCAGAGGGATGAGCCGGAGCCAGGGACTAGGCGGTCCCCGGCGCCCTCGCCGG
  GCGCACGCTGGTTGGGGAGCACCCTGCATGGCCGGGGGCCGCCGGGCTCCCGTAAGCCCGGGGAGGGCGC
  CAGGGCGGAGGCCCTGTGGCCACGGGACGCCCTCCTATTTGCCGTGGACAACCTGAACCGCGTGGAAGGG
  CTGCTACCCTACAACCTGTCTTTGGAAGTAGTGATGGCCATCGAGGCAGGCCTGGGCGATCTGCCACTTT
  TGCCCTTCTCCTCCCCTAGTTCGCCATGGAGCAGTGACCCTTTCTCCTTCCTGCAAAGTGTGTGCCATAC
  CGTGGTGGTGCAAGGGGTGTCGGCGCTGCTCGCCTTCCCCCAGAGCCAGGGCGAAATGATGGAGCTCGAC
  TTGGTCAGCTTAGTCCTGCACATTCCAGTGATCAGCATCGTGCGCCACGAGTTTCCACGGGAGAGTCAGA
  ATCCCCTTCACCTACAACTGAGTTTAGAAAATTCATTAAGTTCTGATGCTGATGTCACTGTCTCAATCCT
  GACCATGAACAACTGGTACAATTTTAGCTTGTTGCTGTGCCAGGAAGACTGGAACATCACCGACTTCCTC
  CTCCTTACCCAGAATAATTCCAAGTTCCACCTTGGTTCTATCATCAACATCACCGCTAACCTCCCCTCCA
  CCCAGGACCTCTTGAGCTTCCTACAGATCCAGCTTGAGAGTATTAAGAACAGCACACCCACAGTGGTGAT
  GTTTGGCTGCGACATGGAAAGTATCCGGCGGATTTTCGAAATTACAACCCAGTTTGGGGTCATGCCCCCT
  GAACTTCGTTGGGTGCTGGGAGATTCCCAGAATGTGGAGGAACTGAGGACAGAGGGTCTGCCCTTAGGGC
  TCATTGCTCATGGAAAAACAACACAGTCTGTCTTTGAGCACTACGTACAAGATGCTATGGAGCTGGTCGC
  AAGAGCTGTAGCCACAGCCACCATGATCCAACCAGAACTTGCTCTCATTCCCAGCACGATGAACTGCATG
  GAGGTGGAAACTACAAATCTCACTTCAGGACAATATTTATCAAGGTTTCTAGCCAATACCACTTTCAGAG
  GCCTCAGTGGTTCCATCAGAGTAAAAGGTTCCACCATCGTCAGCTCAGAAAACAACTTTTTCATCTGGAA
  TCTTCAACATGACCCCATGGGAAAGCCAATGTGGACCCGCTTGGGCAGCTGGCAGGGGGGAAAGATTGTC
  ATGGACTATGGAATATGGCCAGAGCAGGCCCAGAGACACAAAACCCACTTCCAACATCCAAGTAAGCTAC
  ACTTGAGAGTGGTTACCCTGATTGAGCATCCTTTTGTCTTCACAAGGGAGGTAGATGATGAAGGCTTGTG
  CCCTGCTGGCCAACTCTGTCTAGACCCCATGACTAATGACTCTTCCACATTGGACAGCCTTTTTAGCAGC
  CTCCATAGCAGTAATGATACAGTGCCCATTAAATTCAAGAAGTGCTGCTATGGATATTGCATTGATCTGC
  TGGAAAAGATAGCAGAAGACATGAACTTTGACTTCGACCTCTATATTGTAGGGGATGGAAAGTATGGAGC
  ATGGAAAAATGGGCACTGGACTGGGCTAGTGGGTGATCTCCTGAGAGGGACTGCCCACATGGCAGTCACT
  TCCTTTAGCATCAATACTGCACGGAGCCAGGTGATAGATTTCACCAGCCCTTTCTTCTCCACCAGCTTGG
  GCATCTTAGTGAGGACCCGAGATACAGCAGCTCCCATTGGAGCCTTCATGTGGCCACTCCACTGGACAAT
  GTGGCTGGGGATTTTTGTGGCTCTGCACATCACTGCCGTCTTCCTCACTCTGTATGAATGGAAGAGTCCA
  TTTGGTTTGACTCCCAAGGGGCGAAATAGAAGTAAAGTCTTCTCCTTTTCTTCAGCCTTGAACATCTGTT
  ATGCCCTCTTGTTTGGCAGAACAGTGGCCATCAAACCTCCAAAATGTTGGACTGGAAGGTTTCTAATGAA
  CCTTTGGGCCATTTTCTGTATGTTTTGCCTTTCCACATACACGGCAAACTTGGCTGCTGTCATGGTAGGT
  GAGAAGATCTATGAAGAGCTTTCTGGAATACATGACCCCAAGTTACATCATCCTTCCCAAGGATTCCGCT
  TTGGAACTGTCCGAGAAAGCAGTGCTGAAGATTATGTGAGACAAAGTTTCCCAGAGATGCATGAATATAT
  GAGAAGGTACAATGTTCCAGCCACCCCTGATGGAGTGGAGTATCTGAAGAACGATCCAGAGAAACTAGAC
  GCCTTCATCATGGACAAAGCCCTTCTGGATTATGAAGTGTCAATAGATGCTGACTGCAAACTTCTCACTG
  TGGGGAAGCCATTTGCCATAGAAGGATACGGCATTGGCCTCCCACCCAACTCTCCATTGACCGCCAACAT
  ATCCGAGCTAATCAGTCAATACAAGTCACATGGGTTTATGGATATGCTCCATGACAAGTGGTACAGGGTG
  GTTCCCTGTGGCAAGAGAAGTTTTGCTGTCACGGAGACTTTGCAAATGGGCATCAAACACTTCTCTGGGC
  TCTTTGTGCTGCTGTGCATTGGATTTGGTCTGTCCATTTTGACCACCATTGGTGAGCACATAGTATACAG
  GCTGCTGCTACCACGAATCAAAAACAAATCCAAGCTGCAATACTGGCTCCACACCAGCCAGAGATTACAC
  AGAGCAATAAATACATCATTTATAGAGGAAAAGCAGCAGCATTTCAAGACCAAACGTGTGGAAAAGAGGT
  CTAATGTGGGACCCCGTCAGCTTACCGTATGGAATACTTCCAATCTGAGTCATGACAACCGACGGAAATA
  CATCTTTAGTGATGAGGAAGGACAAAACCAGCTGGGCATCCAGATCCACCAGGACATCCCCCTCCCTCCA
  AGGAGAAGAGAGCTCCCTGCCTTGCGGACCACCAATGGGAAAGCAGACTCCCTAAATGTATCTCGGAACT
  CAGTGATGCAGGAACTCTCAGAGCTCGAGAAGCAGATTCAGGTGATCCGTCAGGAGCTGCAGCTGGCTGT
  GAGCAGGAAAACGGAGCTGGAGGAGTATCAAAGGACAAGTCGGACTTGTGAGTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215549 representing NM_133445
  Red=Cloning site Green=Tags(s)
  
  MRRLSLWWLLSRVCLLLPPPCALVLAGVPSSSSHPQPCQILKRIGHAVRVGAVHLQPWTTAPRAASRAPD
  DSRAGAQRDEPEPGTRRSPAPSPGARWLGSTLHGRGPPGSRKPGEGARAEALWPRDALLFAVDNLNRVEG
  LLPYNLSLEVVMAIEAGLGDLPLLPFSSPSSPWSSDPFSFLQSVCHTVVVQGVSALLAFPQSQGEMMELD
  LVSLVLHIPVISIVRHEFPRESQNPLHLQLSLENSLSSDADVTVSILTMNNWYNFSLLLCQEDWNITDFL
  LLTQNNSKFHLGSIINITANLPSTQDLLSFLQIQLESIKNSTPTVVMFGCDMESIRRIFEITTQFGVMPP
  ELRWVLGDSQNVEELRTEGLPLGLIAHGKTTQSVFEHYVQDAMELVARAVATATMIQPELALIPSTMNCM
  EVETTNLTSGQYLSRFLANTTFRGLSGSIRVKGSTIVSSENNFFIWNLQHDPMGKPMWTRLGSWQGGKIV
  MDYGIWPEQAQRHKTHFQHPSKLHLRVVTLIEHPFVFTREVDDEGLCPAGQLCLDPMTNDSSTLDSLFSS
  LHSSNDTVPIKFKKCCYGYCIDLLEKIAEDMNFDFDLYIVGDGKYGAWKNGHWTGLVGDLLRGTAHMAVT
  SFSINTARSQVIDFTSPFFSTSLGILVRTRDTAAPIGAFMWPLHWTMWLGIFVALHITAVFLTLYEWKSP
  FGLTPKGRNRSKVFSFSSALNICYALLFGRTVAIKPPKCWTGRFLMNLWAIFCMFCLSTYTANLAAVMVG
  EKIYEELSGIHDPKLHHPSQGFRFGTVRESSAEDYVRQSFPEMHEYMRRYNVPATPDGVEYLKNDPEKLD
  AFIMDKALLDYEVSIDADCKLLTVGKPFAIEGYGIGLPPNSPLTANISELISQYKSHGFMDMLHDKWYRV
  VPCGKRSFAVTETLQMGIKHFSGLFVLLCIGFGLSILTTIGEHIVYRLLLPRIKNKSKLQYWLHTSQRLH
  RAINTSFIEEKQQHFKTKRVEKRSNVGPRQLTVWNTSNLSHDNRRKYIFSDEEGQNQLGIQIHQDIPLPP
  RRRELPALRTTNGKADSLNVSRNSVMQELSELEKQIQVIRQELQLAVSRKTELEEYQRTSRTCES
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133445
• ORF Size: 3345 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133445.1, NP_597702.1
• RefSeq Size: 7770 bp
• RefSeq ORF: 3348 bp
• Locus ID: 116443
• UniProt ID: Q8TCU5
• Cytogenetics: 9q31.1
• Protein Families: Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: This gene encodes a subunit of the N-methyl-D-aspartate (NMDA) receptors, which belong to the superfamily of glutamate-regulated ion channels, and function in physiological and pathological processes in the central nervous system. This subunit shows greater than 90% identity to the corresponding subunit in rat. Studies in the knockout mouse deficient in this subunit suggest that this gene may be involved in the development of synaptic elements by modulating NMDA receptor activity. [provided by RefSeq, Jul 2008]