GAB2 (NM_012296) Human Tagged ORF Clone

CAT#: RG215374

GAB2 (tGFP-tagged) - Human GRB2-associated binding protein 2 (GAB2), transcript variant 2

Product Images

Circular map for RG215374

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GAB2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215374 representing NM_012296
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGGCGGCGGCGACGTGGTGTGCACCGGCTGGCTGAGGAAATCGCCTCCCGAGAAGAAGTTGAGGC
  GCTATGCCTGGAAGAAACGCTGGTTTATCCTGCGGAGTGGCCGGATGAGCGGTGACCCAGATGTTCTGGA
  ATACTACAAGAACGATCACTCCAAGAAGCCTCTGCGGATCATCAACCTGAACTTCTGTGAGCAGGTAGAT
  GCAGGCCTGACCTTTAACAAGAAGGAGCTGCAGGATAGTTTTGTGTTTGACATCAAGACCAGTGAACGCA
  CCTTTTACCTGGTGGCTGAGACAGAAGAGGACATGAATAAGTGGGTCCAGAGCATCTGCCAGATCTGTGG
  CTTCAATCAGGCTGAGGAGAGCACAGACTCCCTGAGAAATGTTTCCTCAGCCGGTCATGGCCCCCGCTCT
  TCTCCAGCTGAGCTCAGCAGCTCTAGCCAGCACCTTCTCCGAGAGCGCAAGTCCTCAGCCCCATCACACT
  CCAGCCAGCCAACTCTGTTCACGTTTGAACCCCCTGTGTCAAACCACATGCAGCCCACCTTGTCCACCAG
  CGCACCTCAGGAGTATCTCTACTTGCACCAGTGCATAAGCCGAAGAGCAGAAAATGCAAGGAGTGCCAGC
  TTCTCTCAGGGCACCAGAGCCTCTTTTCTCATGAGGAGTGACACAGCTGTACAAAAACTTGCCCAGGGCA
  ATGGACACTGTGTCAACGGGATCAGTGGTCAAGTCCATGGCTTCTATAGCCTTCCCAAGCCGAGCCGGCA
  CAATACAGAATTCAGAGACAGTACCTACGACCTCCCCCGCAGCCTGGCCTCCCATGGCCACACCAAGGGC
  AGCCTCACAGGCTCCGAGACAGATAATGAGGATGTGTACACCTTCAAGACGCCCAGCAACACCCTGTGCA
  GGGAGTTCGGGGACCTCCTGGTAGACAATATGGATGTTCCGGCCACCCCACTCTCAGCCTACCAGATCCC
  TAGGACATTCACTCTGGACAAAAACCACAATGCCATGACAGTGGCCACTCCTGGGGACTCAGCCATAGCT
  CCCCCACCCCGCCCCCCCAAGCCAAGTCAGGCAGAAACACCTCGATGGGGCAGTCCTCAGCAGAGACCGC
  CAATCAGTGAAAATAGCAGATCTGTCGCTGCCACCATCCCCAGACGCAACACCCTCCCTGCAATGGACAA
  CAGCCGACTTCACCGAGCTTCTTCCTGTGAGACCTACGAGTACCCACAGCGTGGTGGAGAGAGTGCAGGC
  CGGTCTGCTGAATCCATGAGTGATGGAGTTGGCTCTTTCCTGCCAGGGAAAATGATTGTGGGCCGATCGG
  ACAGCACCAATTCTGAAGACAACTATGTGCCCATGAATCCAGGTTCTTCCACCCTGTTGGCCATGGAACG
  AGCAGGTGATAATTCCCAGAGCGTCTACATCCCAATGAGCCCAGGGGCCCATCACTTTGACTCACTTGGC
  TACCCATCAACAACCCTTCCTGTGCACCGAGGCCCCAGCAGAGGAAGTGAGATTCAGCCACCCCCTGTCA
  ACCGCAACCTCAAACCTGATCGGAAAGCAAAGCCAACACCACTTGACCTGAGGAACAACACCGTCATCGA
  TGAACTCCCCTTCAAGTCACCTATCACCAAGTCTTGGTCTAGGGCCAACCACACCTTCAACTCCAGCTCC
  TCCCAGTACTGCCGCCCCATCTCCACCCAGAGCATCACCAGCACAGACTCAGGAGACAGCGAAGAGAACT
  ATGTCCCTATGCAAAACCCAGTGTCTGCATCTCCCGTTCCCAGTGGCACGAACAGTCCTGCCCCTAAGAA
  GAGCACCGGCAGCGTTGATTATCTGGCCCTGGACTTCCAGCCGAGCTCCCCAAGCCCCCACCGCAAGCCA
  TCTACTTCATCCGTCACCTCTGATGAGAAGGTGGACTACGTTCAGGTGGACAAGGAGAAGACCCAGGCCC
  TGCAGAACACCATGCAGGAGTGGACAGACGTGCGGCAGTCCTCAGAGCCTTCCAAGGGTGCCAAGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215374 representing NM_012296
  Red=Cloning site Green=Tags(s)
  
  MSGGGDVVCTGWLRKSPPEKKLRRYAWKKRWFILRSGRMSGDPDVLEYYKNDHSKKPLRIINLNFCEQVD
  AGLTFNKKELQDSFVFDIKTSERTFYLVAETEEDMNKWVQSICQICGFNQAEESTDSLRNVSSAGHGPRS
  SPAELSSSSQHLLRERKSSAPSHSSQPTLFTFEPPVSNHMQPTLSTSAPQEYLYLHQCISRRAENARSAS
  FSQGTRASFLMRSDTAVQKLAQGNGHCVNGISGQVHGFYSLPKPSRHNTEFRDSTYDLPRSLASHGHTKG
  SLTGSETDNEDVYTFKTPSNTLCREFGDLLVDNMDVPATPLSAYQIPRTFTLDKNHNAMTVATPGDSAIA
  PPPRPPKPSQAETPRWGSPQQRPPISENSRSVAATIPRRNTLPAMDNSRLHRASSCETYEYPQRGGESAG
  RSAESMSDGVGSFLPGKMIVGRSDSTNSEDNYVPMNPGSSTLLAMERAGDNSQSVYIPMSPGAHHFDSLG
  YPSTTLPVHRGPSRGSEIQPPPVNRNLKPDRKAKPTPLDLRNNTVIDELPFKSPITKSWSRANHTFNSSS
  SQYCRPISTQSITSTDSGDSEENYVPMQNPVSASPVPSGTNSPAPKKSTGSVDYLALDFQPSSPSPHRKP
  STSSVTSDEKVDYVQVDKEKTQALQNTMQEWTDVRQSSEPSKGAKL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012296
• ORF Size: 2028 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012296.2, NP_036428.1
• RefSeq Size: 6053 bp
• RefSeq ORF: 1917 bp
• Locus ID: 9846
• UniProt ID: Q9UQC2
• Cytogenetics: 11q14.1
• Protein Families: Druggable Genome
• Protein Pathways: Chronic myeloid leukemia, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis
• Gene Summary: This gene is a member of the GRB2-associated binding protein (GAB) gene family. These proteins contain pleckstrin homology (PH) domain, and bind SHP2 tyrosine phosphatase and GRB2 adapter protein. They act as adapters for transmitting various signals in response to stimuli through cytokine and growth factor receptors, and T- and B-cell antigen receptors. The protein encoded by this gene is the principal activator of phosphatidylinositol-3 kinase in response to activation of the high affinity IgE receptor. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Nov 2009]