UGT2B15 (NM_001076) Human Tagged ORF Clone

CAT#: RG215344

UGT2B15 (tGFP-tagged) - Human UDP glucuronosyltransferase 2 family, polypeptide B15 (UGT2B15)

Product Images

Circular map for RG215344

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: UGT2B15
• Synonyms: HLUG4; UDPGT 2B8; UDPGT2B15; UDPGTH3; UGT2B8
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215344 representing NM_001076
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCTGAAATGGACGTCAGTCTTTCTGCTGATACAGCTCAGTTGTTACTTTAGCTCTGGAAGCTGTG
  GAAAGGTGCTAGTGTGGCCCACAGAATACAGCCATTGGATAAATATGAAGACAATCCTGGAAGAGCTTGT
  TCAGAGGGGTCATGAGGTGACTGTGTTGACATCTTCGGCTTCTACTCTTGTCAATGCCAGTAAATCATCT
  GCTATTAAATTAGAAGTTTATCCTACATCTTTAACTAAAAATTATTTGGAAGATTCTCTTCTGAAAATTC
  TCGATAGATGGATATATGGTGTTTCAAAAAATACATTTTGGTCATATTTTTCACAATTACAAGAATTGTG
  TTGGGAATATTATGACTACAGTAACAAGCTCTGTAAAGATGCAGTTTTGAATAAGAAACTTATGATGAAA
  CTACAAGAGTCAAAGTTTGATGTCATTCTGGCAGATGCCCTTAATCCCTGTGGTGAGCTACTGGCTGAAC
  TATTTAACATACCCTTTCTGTACAGTCTTCGATTCTCTGTTGGCTACACATTTGAGAAGAATGGTGGAGG
  ATTTCTGTTCCCTCCTTCCTATGTACCTGTTGTTATGTCAGAATTAAGTGATCAAATGATTTTCATGGAG
  AGGATAAAAAATATGATACATATGCTTTATTTTGACTTTTGGTTTCAAATTTATGATCTGAAGAAGTGGG
  ACCAGTTTTATAGTGAAGTTCTAGGAAGACCCACTACATTATTTGAGACAATGGGGAAAGCTGAAATGTG
  GCTCATTCGAACCTATTGGGATTTTGAATTTCCTCGCCCATTCTTACCAAATGTTGATTTTGTTGGAGGA
  CTTCACTGTAAACCAGCCAAACCCCTGCCTAAGGAAATGGAAGAGTTTGTGCAGAGCTCTGGAGAAAATG
  GTATTGTGGTGTTTTCTCTGGGGTCGATGATCAGTAACATGTCAGAAGAAAGTGCCAACATGATTGCATC
  AGCCCTTGCCCAGATCCCACAAAAGGTTCTATGGAGATTTGATGGCAAGAAGCCAAATACTTTAGGTTCC
  AATACTCGACTGTACAAGTGGTTACCCCAGAATGACCTTCTTGGTCATCCCAAAACCAAAGCTTTTATAA
  CTCATGGTGGAACCAATGGCATCTATGAGGCGATCTACCATGGGATCCCTATGGTGGGCATTCCCTTGTT
  TGCGGATCAACATGATAACATTGCTCACATGAAAGCCAAGGGAGCAGCCCTCAGTGTGGACATCAGGACC
  ATGTCAAGTAGAGATTTGCTCAATGCATTGAAGTCAGTCATTAATGACCCTGTCTATAAAGAGAATGTCA
  TGAAATTATCAAGAATTCATCATGACCAACCAATGAAGCCCCTGGATCGAGCAGTCTTCTGGATTGAGTT
  TGTCATGCGCCACAAAGGAGCCAAGCACCTTCGAGTCGCAGCTCACAACCTCACCTGGATCCAGTACCAC
  TCTTTGGATGTGATAGCATTCCTGCTGGCCTGCGTGGCAACTGTGATATTTATCATCACAAAATTTTGCC
  TGTTTTGTTTCCGAAAGCTTGCCAAAAAAGGAAAGAAGAAGAAAAGAGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215344 representing NM_001076
  Red=Cloning site Green=Tags(s)
  
  MSLKWTSVFLLIQLSCYFSSGSCGKVLVWPTEYSHWINMKTILEELVQRGHEVTVLTSSASTLVNASKSS
  AIKLEVYPTSLTKNYLEDSLLKILDRWIYGVSKNTFWSYFSQLQELCWEYYDYSNKLCKDAVLNKKLMMK
  LQESKFDVILADALNPCGELLAELFNIPFLYSLRFSVGYTFEKNGGGFLFPPSYVPVVMSELSDQMIFME
  RIKNMIHMLYFDFWFQIYDLKKWDQFYSEVLGRPTTLFETMGKAEMWLIRTYWDFEFPRPFLPNVDFVGG
  LHCKPAKPLPKEMEEFVQSSGENGIVVFSLGSMISNMSEESANMIASALAQIPQKVLWRFDGKKPNTLGS
  NTRLYKWLPQNDLLGHPKTKAFITHGGTNGIYEAIYHGIPMVGIPLFADQHDNIAHMKAKGAALSVDIRT
  MSSRDLLNALKSVINDPVYKENVMKLSRIHHDQPMKPLDRAVFWIEFVMRHKGAKHLRVAAHNLTWIQYH
  SLDVIAFLLACVATVIFIITKFCLFCFRKLAKKGKKKKRD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001076
• ORF Size: 1590 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001076.4
• RefSeq Size: 2144 bp
• RefSeq ORF: 1593 bp
• Locus ID: 7366
• UniProt ID: P54855
• Cytogenetics: 4q13.2
• Domains: UDPGT
• Protein Families: Transmembrane
• Protein Pathways: Androgen and estrogen metabolism, Ascorbate and aldarate metabolism, Drug metabolism - cytochrome P450, Drug metabolism - other enzymes, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Pentose and glucuronate interconversions, Porphyrin and chlorophyll metabolism, Retinol metabolism, Starch and sucrose metabolism
• Gene Summary: This gene encodes a glycosyltransferase that is invovled in the metabolism and elimination of toxic compounts, both endogenous and of xenobiotic origin. This gene plays a role in the regulation of estrogens and androgens. This locus is present in a cluster of similar genes and pseudogenes on chromosome 4. [provided by RefSeq, Aug 2016]