FHDC1 (NM_033393) Human Tagged ORF Clone

CAT#: RG214806

FHDC1 (tGFP-tagged) - Human FH2 domain containing 1 (FHDC1)

Product Images

Circular map for RG214806

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: FHDC1
• Synonyms: INF1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214806 representing NM_033393
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATGTTATGAATTGTGTCTCCTTGGTCAGTGATAAAGAAAATGGGAATATTGCCACAGCACCTGGAT
  TCATGATTGGGCAGACACCTCCTCCAGCACCTCCTCCACCTCCTCCTCCACCCCCTCCATCTCCACCATG
  TTCATGTTCAAGGGAAGAGTGTCCTTCCTCCCCTCCTCCACCCCCACCACCTCCACTTCCTGGGGAGCCT
  CCCATCCCACCTCCCCCACCAGGCCTACCCCCAACTACTCACATGAACGGCTACAGCCACCTTGGTAAGA
  AAAAGCGGATGAGAAGCTTTTTTTGGAAAACTATTCCGGAGGAGCAAGTTCGAGGCAAAACCAACATCTG
  GACCTTGGCAGCCAGGCAGGAACATCACTACCAAATTGATACAAAGACCATTGAGGAGCTCTTTGGGCAG
  CAGGAAGACACCACCAAGTCTTCCCTTCCTAGGAGAGGAAGAACTTTAAATTCATCCTTCAGAGAAGCTC
  GAGAAGAGATTACTATTTTGGATGCAAAACGGAGCATGAACATTGGGATATTTCTTAAGCAATTTAAGAA
  GTCTCCTCGGTCCATTGTAGAAGATATTCATCAAGGAAAAAGTGAGCATTATGGATCAGAGACCTTGCGA
  GAATTTCTTAAGTTTTTGCCAGAGTCAGAAGAGGTAAAGAAGTTAAAAGCGTTTAGTGGCGACGTGTCGA
  AGCTGTCTCTGGCAGATTCCTTTCTGTATGGCTTAATTCAGGTGCCAAACTATTCACTTCGGATTGAAGC
  CATGGTGCTAAAGAAGGAATTTCTACCTTCTTGCTCTTCTCTATATACAGATATAACAGTTTTAAGAACT
  GCTATAAAAGAACTGATGTCATGTGAAGAGCTACATTCAATATTACACTTGGTGCTCCAGGCTGGGAATA
  TCATGAATGCAGGAGGGTATGCCGGCAATGCAGTAGGATTTAAACTGTCTTCTTTGCTCAAATTGGCAGA
  CACAAAAGCAAACAAACCTGGGATGAATCTCCTGCACTTTGTTGCACAGGAAGCCCAAAAGAAAGATACC
  ATTCTTCTAAACTTTTCAGAAAAATTGCATCATGTTCAGAAGACTGCTAGATTATCTCTGGAGAACACGG
  AGGCAGAACTGCACTTGCTGTTTGTCAGGACAAAATCACTAAAAGAAAACATCCAGCGGGATGGTGAACT
  TTGTCAGCAGATGGAAGATTTTCTTCAGTTTGCCATAGAAAAGCTGAGGGAACTGGAATGCTGGAAACAA
  GAGCTCCAGGATGAGGCCTACACCCTTATAGATTTTTTCTGTGAAGACAAAAAAACCATGAAACTGGATG
  AATGCTTTCAGATATTTAGAGATTTCTGTACCAAATTCAACAAAGCAGTTAAGGACAACCACGACCGGGA
  GGCGCAGGAGCTGAGGCAGCTGCAGAGGCTGAAGGAGCAGGAGCAGAAGCAGCGCTCCTGGGCAACTGGG
  GAGCTGGGGGCATTTGGCCGGAGCAGCAGTGAGAATGATGTGGAGCTGCTGACCAAGAAGGGTGCAGAGG
  GCCTGCTCCCTTTCCTGCACCCCAGGCCCATCAGCCCCTCCAGCCCCTCCTACCGGCCCCCGAACACCCG
  CCGCTCCCGCCTCTCCCTGGGTCCCTCTGCTGACCGGGAGCTGCTGACCTTCTTGGAGAGCTCCACCGGC
  AGCCCTGAGGAGCCCAATAAGTTCCACAGCCTGCCCCGGAGCAGCCCCCGGCAGGCCCGGCCCACGATAG
  CCTGCCTGGAGCCTGCAGAAGTGAGGCACCAGGACTCCAGCTTTGCACACAAACCTCAGGCCTCGGGGGG
  CCAGGAGGAGGCCCCCAACCCACCCTCAGCACAGGCGCACCAGCTTGCAGCCGCCCAGCCTGAGAACCAT
  GCCTCTGCCTTCCCCAGAGCTCGGTGCCAGGGCGTCAGTGTCCTCCGAAAGAGGTACAGTGAGCCGGTGA
  GCCTGGGCTCAGCACAGTCCCCTCCTCTCTCGCCATTGGCTCTGGGAATTAAGGAGCATGAGCTGGTGAC
  AGGGCTGGCCCAGTTCAACCTCCAGGGTTCCCAGGGCATGGAGGAGACCTCCCAGCTGACTCTGAGTGAC
  TTCAGCCCGATGGAGCTAGAGTCTGTGGGGCATAGGGGCCCGCAGTCCCTCAGTGCCAGCAGCAGCAGCC
  TGACACCCATGGGCAGAGATGCCCTGGGGAGTCTCAGCCCAGCGCTGGAGGATGGCAAGGCTGCCCCCGA
  TGAGCCTGGAAGTGCAGCTTTGGGATCTGTGGGTAGCAGCGACCCTGAGAACAAAGATCCTAGACCTCTG
  TTCTGCATCTCGGACACCACCGACTGCTCACTGACCCTGGACTGCTCAGAGGGAACCGACTCCAGACCCA
  GAGGCGGGGACCCGGAGGAAGGCGGGGAAGGGGATGGCTCCATGTCCTCTGGGGTTGGAGAAATGGGGGA
  CAGCCAAGTCTCCTCCAACCCTACATCCAGCCCCCCTGGGGAGGCTCCTGCCCCCGTCTCTGTGGATAGT
  GAGCCCAGCTGCAAGGGCGGCCTGCCCAGGGACAAACCCACCAAAAGGAAAGATGTTGTAGCACCAAAGA
  GAGGCTCCCTGAAAGAGGCGTCTCCCGGGGCCTCCAAGCCCGGGAGCGCCCGGCGGAGCCAGGGGGCAGT
  GGCCAAGTCTGTGCGGACCCTGACCGCCTCAGAGAACGAGAGCATGCGCAAGGTCATGCCCATCACCAAG
  TCCAGCAGAGGCGCCGGCTGGAGGCGACCAGAGCTGTCATCCCGGGGGCCCTCCCAGAATCCCCCCAGCA
  GCACAGATACTGTGTGGTCACGCCAGAACTCCGTGCGGAGGGCCTCCACAGGCGCCGAAGAGCAGAGGCT
  GCCGCGGGGGAGCAGCGGCTCCAGCAGCACCCGTCCGGGGAGGGACGTTCCCCTGCAGCCCAGGGGTTCT
  TTCAAGAAGCCCAGTGCCAAACCACTCAGGAACCTCCCCAGACAGAAGCCTGAGGAAAATAAGACCTGCC
  GCGCCCACTCCGAGGGCCCTGAGAGTCCCAAAGAAGAGCCCAAGACCCCGTCAGTGCCCAGCGTCCCCCA
  CGAACTACCCCGTGTCCCGAGCTTTGCCCGGAACACAGTGGCCTCCTCCTCTCGAAGCATGAGAACAGAT
  CTTCCTCCCGTGGCCAAAGCCCCCGGCATCACTCGGACAGTGTCGCAGCGGCAGCTGAGGGTGAAAGGGG
  ACCCCGAGGATGCCGCTCCCAAGGACAGCAGCACTTTGAGGCGAGCCAGCAGTGCCCGGGCCCCCAAGAA
  GCGCCCAGAGTCTGCGGAGGGTCCCAGTGCCAACACGGAGGCCCCTCTGAAGGCCAGAGGGGCTGGGGAA
  AGGGCCTCCCTCCGTCGGAAGGACTCCAGTCGGACCACGCTGGGGAGAATCCTCAATCCCTTACGGAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214806 representing NM_033393
  Red=Cloning site Green=Tags(s)
  
  MHVMNCVSLVSDKENGNIATAPGFMIGQTPPPAPPPPPPPPPPSPPCSCSREECPSSPPPPPPPPLPGEP
  PIPPPPPGLPPTTHMNGYSHLGKKKRMRSFFWKTIPEEQVRGKTNIWTLAARQEHHYQIDTKTIEELFGQ
  QEDTTKSSLPRRGRTLNSSFREAREEITILDAKRSMNIGIFLKQFKKSPRSIVEDIHQGKSEHYGSETLR
  EFLKFLPESEEVKKLKAFSGDVSKLSLADSFLYGLIQVPNYSLRIEAMVLKKEFLPSCSSLYTDITVLRT
  AIKELMSCEELHSILHLVLQAGNIMNAGGYAGNAVGFKLSSLLKLADTKANKPGMNLLHFVAQEAQKKDT
  ILLNFSEKLHHVQKTARLSLENTEAELHLLFVRTKSLKENIQRDGELCQQMEDFLQFAIEKLRELECWKQ
  ELQDEAYTLIDFFCEDKKTMKLDECFQIFRDFCTKFNKAVKDNHDREAQELRQLQRLKEQEQKQRSWATG
  ELGAFGRSSSENDVELLTKKGAEGLLPFLHPRPISPSSPSYRPPNTRRSRLSLGPSADRELLTFLESSTG
  SPEEPNKFHSLPRSSPRQARPTIACLEPAEVRHQDSSFAHKPQASGGQEEAPNPPSAQAHQLAAAQPENH
  ASAFPRARCQGVSVLRKRYSEPVSLGSAQSPPLSPLALGIKEHELVTGLAQFNLQGSQGMEETSQLTLSD
  FSPMELESVGHRGPQSLSASSSSLTPMGRDALGSLSPALEDGKAAPDEPGSAALGSVGSSDPENKDPRPL
  FCISDTTDCSLTLDCSEGTDSRPRGGDPEEGGEGDGSMSSGVGEMGDSQVSSNPTSSPPGEAPAPVSVDS
  EPSCKGGLPRDKPTKRKDVVAPKRGSLKEASPGASKPGSARRSQGAVAKSVRTLTASENESMRKVMPITK
  SSRGAGWRRPELSSRGPSQNPPSSTDTVWSRQNSVRRASTGAEEQRLPRGSSGSSSTRPGRDVPLQPRGS
  FKKPSAKPLRNLPRQKPEENKTCRAHSEGPESPKEEPKTPSVPSVPHELPRVPSFARNTVASSSRSMRTD
  LPPVAKAPGITRTVSQRQLRVKGDPEDAAPKDSSTLRRASSARAPKKRPESAEGPSANTEAPLKARGAGE
  RASLRRKDSSRTTLGRILNPLRK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033393
• ORF Size: 3429 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033393.1, NP_203751.1
• RefSeq Size: 6470 bp
• RefSeq ORF: 3432 bp
• Locus ID: 85462
• UniProt ID: Q9C0D6
• Cytogenetics: 4q31.3
• Gene Summary: Microtubule-associated formin which regulates both actin and microtubule dynamics. Induces microtubule acetylation and stabilization and actin stress fiber formation (PubMed:18815276). Regulates Golgi ribbon formation (PubMed:26564798). Required for normal cilia assembly. Early in cilia assembly, may assist in the maturation and positioning of the centrosome/basal body, and once cilia assembly has initiated, may also promote cilia elongation by inhibiting disassembly (PubMed:29742020).[UniProtKB/Swiss-Prot Function]