C2GNT3 (GCNT4) (NM_016591) Human Tagged ORF Clone

CAT#: RG214560

GCNT4 (tGFP-tagged) - Human glucosaminyl (N-acetyl) transferase 4, core 2 (GCNT4)

Product Images

Circular map for RG214560

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: C2GNT3
• Synonyms: C2GNT3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214560 representing NM_016591
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATATTCAAATGTTATTTTAAACATACCCTACAGCAGAAAGTTTTCATCCTGTTTTTAACCCTAT
  GGCTGCTCTCTTTGTTAAAGCTTCTAAATGTGAGACGACTCTTTCCGCAAAAAGACATTTACTTGGTTGA
  GTACTCCCTAAGTACCTCGCCTTTTGTAAGAAACAGATACACTCATGTTAAGGATGAAGTCAGGTATGAA
  GTTAACTGTTCGGGTATCTATGAACAGGAGCCTTTGGAAATTGGAAAGAGTCTGGAAATAAGAAGAAGGG
  ACATCATTGACTTGGAGGATGATGATGTTGTGGCAATGACCAGTGATTGTGACATTTATCAGACTCTAAG
  AGGTTATGCTCAAAAGCTTGTCTCAAAGGAGGAGAAAAGCTTCCCAATAGCCTATTCTTTGGTTGTCCAC
  AAAGATGCAATTATGGTTGAAAGGCTTATCCATGCTATATACAACCAGCACAATATTTACTGCATCCATT
  ATGATCGTAAGGCACCTGATACCTTCAAAGTTGCCATGAACAATTTAGCTAAGTGCTTCTCCAATATTTT
  CATTGCTTCCAAATTAGAGGCTGTGGAATATGCCCACATTTCCAGACTCCAGGCTGATTTAAATTGCTTG
  TCGGACCTTCTGAAGTCTTCAATCCAGTGGAAATATGTTATCAACTTGTGTGGGCAAGATTTTCCCCTGA
  AGTCAAATTTTGAATTGGTGTCAGAGTTGAAAAAACTCAATGGAGCAAATATGTTGGAGACGGTGAAACC
  CCCAAACAGTAAATTGGAAAGATTCACTTACCATCATGAACTTAGACGGGTGCCTTATGAATATGTGAAG
  CTACCAATAAGGACAAACATCTCCAAGGAAGCACCCCCCCATAACATTCAGATATTTGTTGGCAGTGCTT
  ATTTTGTTTTAAGTCAAGCATTTGTTAAATATATTTTCAACAACTCCATCGTTCAAGACTTTTTTGCCTG
  GTCTAAAGACACATACTCTCCTGATGAGCACTTTTGGGCTACCTTGATTCGGGTTCCAGGAATACCTGGG
  GAGATTTCCAGATCAGCCCAGGATGTGTCTGATCTGCAGAGTAAGACTCGCCTTGTCAAGTGGAATTACT
  ATGAAGGCTTTTTCTATCCCAGTTGTACTGGATCTCACCTTCGAAGCGTGTGTATTTATGGAGCTGCAGA
  ATTAAGGTGGCTTATCAAAGATGGACATTGGTTTGCTAATAAATTTGATTCTAAGGTGGACCCTATCTTG
  ATTAAATGCTTGGCAGAAAAGCTTGAAGAACAGCAGAGAGACTGGATCACTTTGCCCTCAGAAAAGTTAT
  TTATGGATAGAAATCTCACTACCACATCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214560 representing NM_016591
  Red=Cloning site Green=Tags(s)
  
  MKIFKCYFKHTLQQKVFILFLTLWLLSLLKLLNVRRLFPQKDIYLVEYSLSTSPFVRNRYTHVKDEVRYE
  VNCSGIYEQEPLEIGKSLEIRRRDIIDLEDDDVVAMTSDCDIYQTLRGYAQKLVSKEEKSFPIAYSLVVH
  KDAIMVERLIHAIYNQHNIYCIHYDRKAPDTFKVAMNNLAKCFSNIFIASKLEAVEYAHISRLQADLNCL
  SDLLKSSIQWKYVINLCGQDFPLKSNFELVSELKKLNGANMLETVKPPNSKLERFTYHHELRRVPYEYVK
  LPIRTNISKEAPPHNIQIFVGSAYFVLSQAFVKYIFNNSIVQDFFAWSKDTYSPDEHFWATLIRVPGIPG
  EISRSAQDVSDLQSKTRLVKWNYYEGFFYPSCTGSHLRSVCIYGAAELRWLIKDGHWFANKFDSKVDPIL
  IKCLAEKLEEQQRDWITLPSEKLFMDRNLTTTS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016591
• ORF Size: 1359 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016591.2, NP_057675.1
• RefSeq Size: 3435 bp
• RefSeq ORF: 1362 bp
• Locus ID: 51301
• Cytogenetics: 5q13.3
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, O-Glycan biosynthesis
• Gene Summary: Glycosyltransferase that mediates core 2 O-glycan branching, an important step in mucin-type biosynthesis. Does not have core 4 O-glycan or I-branching enzyme activity.[UniProtKB/Swiss-Prot Function]