PDE8B (NM_001029852) Human Tagged ORF Clone

CAT#: RG214305

PDE8B (tGFP-tagged) - Human phosphodiesterase 8B (PDE8B), transcript variant 5

Product Images

Circular map for RG214305

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PDE8B
• Synonyms: ADSD; PPNAD3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214305 representing NM_001029852
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTGCGCCCCCAGCATCCATGTCTCGCAGAGCGGCGTGATCTACTGCCGGGACTCGGACGAGTCCA
  GCTCGCCCCGCCAGACCACCAGCGTGTCGCAGGGCCCGGCGGCACCCCTGCCCGGCCTCTTCGTCCAGAC
  CGACGCCGCCGACGCCATCCCCCCGAGCCGCGCGTCGGGACCCCCCAGCGTAGCCCGCGTCCGCAGGGCC
  CGCACCGAGCTGGGCAGCGGTAGCAGCGCGGGTTCCGCAGCCCCCGCCGCGACCACCAGCAGGGGCCGGA
  GGCGCCACTGCTGCAGCAGCGCCGAGGCCGAGACTCAGACCTGCTACACCAGCGTGAAGCAGGTGTCTTC
  TGCGGAGGTGCGCATCGGGCCCATGAGACTGACGCAGGACCCTATTCAGGTTTTGCTGATCTTTGCAAAG
  GAAGATAGTCAGAGCGATGGCTTCTGGTGGGCCTGCGACAGAGCTGGTTATAGATGCAATATTGCTCGGA
  CTCCAGAGTCAGCCCTTGAATGCTTTCTTGATAAGCATCATGAAATTATTGTAATTGATCATAGACAAAC
  TCAGAACTTCGATGCAGAAGCAGTGTGCAGGTCGATCCGGGCCACAAATCCCTCCGAGCACACGGTGATC
  CTCGCAGTGGTTTCGCGAGTATCGGATGACCATGAAGAGGCGTCAGTCCTTCCTCTTCTCCACGCAGGCT
  TCAACAGGAGATTTATGGAGAATAGCAGCATAATTGCTTGCTATAATGAACTGATTCAAATAGAACATGG
  GGAAGTTCGCTCCCAGTTCAAATTACGGGCCTGTAATTCAGTGTTTACAGCATTAGATCACTGTCATGAA
  GCCATAGAAATAACAAGCGATGACCACGTGATTCAGTATGTCAACCCAGCCTTCGAAAGGATGATGGGCT
  ACCACAAAGGTGAGCTCCTGGGAAAAGAACTCGCTGATCTGCCCAAAAGCGATAAGAACCGGGCAGACCT
  TCTCGACACCATCAATACATGCATCAAGAAGGGAAAGGAGTGGCAGGGGGTTTACTATGCCAGACGGAAA
  TCCGGGGACAGCATCCAACAGCACGTGAAGATCACCCCAGTGATTGGCCAAGGAGGGAAAATTAGGCATT
  TTGTCTCGCTCAAGAAACTGTGTTGTACCACTGACAATAATAAGCAGATTCACAAGATTCATCGTGATTC
  AGGAGACAATTCTCAGACAGAGCCTCATTCATTCAGATATAAGAACAGGAGGAAAGAGTCCATTGACGTG
  AAATCGATATCATCTCGAGGCAGTGATGCACCAAGCCTGCAGAATCGTCGCTATCCGTCCATGGCGAGGA
  TCCACTCCATGACCATCGAGGCTCCCATCACAAAGGACGGCTTGAGAAGACTGTCAGGAAACGAGTATGT
  GTTTACTAAGAATGTGCACCAGAGTCACAGTCACCTTGCAATGCCAATAACCATCAATGATGTTCCCCCT
  TGTATCTCTCAATTACTTGATAATGAGGAGAGTTGGGACTTCAACATCTTTGAATTGGAAGCCATTACGC
  ATAAAAGGCCATTGGTTTATCTGGGCTTAAAGGTCTTCTCTCGGTTTGGAGTATGTGAATTTTTAAACTG
  TTCTGAAACCACTCTTCGGGCCTGGTTCCAAGTGATCGAAGCCAACTACCACTCTTCCAATGCCTACCAC
  AACTCCACCCATGCTGCCGACGTCCTGCACGCCACCGCTTTCTTTCTTGGAAAGGAAAGAGTAAAGGGAA
  GCCTCGATCAGTTGGATGAGGTGGCAGCCCTCATTGCTGCCACAGTCCATGACGTGGATCACCCGGGAAG
  GACCAACTCTTTCCTCTGCAATGCAGGCAGTGAGCTTGCTGTGCTCTACAATGACACTGCTGTTCTGGAG
  AGTCACCACACCGCCCTGGCCTTCCAGCTCACGGTCAAGGACACCAAATGCAACATTTTCAAGAATATTG
  ACAGGAACCATTATCGAACGCTGCGCCAGGCTATTATTGACATGGTTTTGGCAACAGAGATGACAAAACA
  CTTTGAACATGTGAATAAGTTTGTGAACAGCATCAACAAGCCAATGGCAGCTGAGATTGAAGGCAGCGAC
  TGTGAATGCAACCCTGCTGGGAAGAACTTCCCTGAAAACCAAATCCTGATCAAACGCATGATGATTAAGT
  GTGCTGACGTGGCCAACCCATGCCGCCCCTTGGACCTGTGCATTGAATGGGCTGGGAGGATCTCTGAGGA
  GTATTTTGCACAGACTGATGAAGAGAAGAGACAGGGACTACCTGTGGTGATGCCAGTGTTTGACCGGAAT
  ACCTGTAGCATCCCCAAGTCTCAGATCTCTTTCATTGACTACTTCATAACAGACATGTTTGATGCTTGGG
  ATGCCTTTGCACATCTGCCAGCCCTGATGCAACATTTGGCTGACAACTACAAACACTGGAAGACACTAGA
  TGACCTAAAGTGCAAAAGTTTGAGGCTTCCATCTGACAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214305 representing NM_001029852
  Red=Cloning site Green=Tags(s)
  
  MGCAPSIHVSQSGVIYCRDSDESSSPRQTTSVSQGPAAPLPGLFVQTDAADAIPPSRASGPPSVARVRRA
  RTELGSGSSAGSAAPAATTSRGRRRHCCSSAEAETQTCYTSVKQVSSAEVRIGPMRLTQDPIQVLLIFAK
  EDSQSDGFWWACDRAGYRCNIARTPESALECFLDKHHEIIVIDHRQTQNFDAEAVCRSIRATNPSEHTVI
  LAVVSRVSDDHEEASVLPLLHAGFNRRFMENSSIIACYNELIQIEHGEVRSQFKLRACNSVFTALDHCHE
  AIEITSDDHVIQYVNPAFERMMGYHKGELLGKELADLPKSDKNRADLLDTINTCIKKGKEWQGVYYARRK
  SGDSIQQHVKITPVIGQGGKIRHFVSLKKLCCTTDNNKQIHKIHRDSGDNSQTEPHSFRYKNRRKESIDV
  KSISSRGSDAPSLQNRRYPSMARIHSMTIEAPITKDGLRRLSGNEYVFTKNVHQSHSHLAMPITINDVPP
  CISQLLDNEESWDFNIFELEAITHKRPLVYLGLKVFSRFGVCEFLNCSETTLRAWFQVIEANYHSSNAYH
  NSTHAADVLHATAFFLGKERVKGSLDQLDEVAALIAATVHDVDHPGRTNSFLCNAGSELAVLYNDTAVLE
  SHHTALAFQLTVKDTKCNIFKNIDRNHYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPMAAEIEGSD
  CECNPAGKNFPENQILIKRMMIKCADVANPCRPLDLCIEWAGRISEEYFAQTDEEKRQGLPVVMPVFDRN
  TCSIPKSQISFIDYFITDMFDAWDAFAHLPALMQHLADNYKHWKTLDDLKCKSLRLPSDS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001029852
• ORF Size: 2490 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001029852.4
• RefSeq Size: 4240 bp
• RefSeq ORF: 2493 bp
• Locus ID: 8622
• UniProt ID: O95263
• Cytogenetics: 5q13.3
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: The protein encoded by this gene is a cyclic nucleotide phosphodiesterase (PDE) that catalyzes the hydrolysis of the second messenger cAMP. The encoded protein, which does not hydrolyze cGMP, is resistant to several PDE inhibitors. Defects in this gene are a cause of autosomal dominant striatal degeneration (ADSD). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2010]