SOS1 (NM_005633) Human Tagged ORF Clone

CAT#: RG213531

SOS1 (tGFP-tagged) - Human son of sevenless homolog 1 (Drosophila) (SOS1)

Product Images

Circular map for RG213531

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SOS1
• Synonyms: GF1; GGF1; GINGF; HGF; NS4; SOS-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213531 representing NM_005633
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGCGCAGCAGCTGCCCTACGAGTTTTTCAGCGAAGAGAACGCGCCCAAGTGGCGGGGACTACTGG
  TGCCTGCGCTGAAAAAGGTCCAGGGGCAAGTTCATCCTACTCTCGAGTCTAATGATGATGCTCTTCAGTA
  TGTTGAAGAATTAATTTTGCAATTATTAAATATGCTATGCCAAGCTCAGCCCCGAAGTGCTTCAGATGTA
  GAGGAACGTGTTCAAAAAAGTTTCCCTCATCCAATTGATAAATGGGCAATAGCTGATGCCCAATCAGCTA
  TTGAAAAGAGGAAGCGAAGAAACCCTTTATCTCTCCCAGTAGAAAAAATTCATCCTTTATTAAAGGAGGT
  CCTAGGTTATAAAATTGACCACCAGGTTTCTGTTTACATAGTAGCAGTCTTAGAATACATTTCTGCAGAC
  ATTTTAAAGCTGGTTGGGAATTATGTAAGAAATATACGGCATTATGAAATTACAAAACAAGATATTAAAG
  TGGCAATGTGTGCTGACAAGGTATTGATGGATATGTTTCATCAAGATGTAGAAGATATTAATATATTATC
  TTTAACTGACGAAGAGCCTTCCACCTCAGGAGAACAAACTTACTATGATTTGGTAAAAGCATTTATGGCA
  GAAATTCGACAATATATAAGGGAACTAAATCTAATTATAAAAGTTTTTAGAGAGCCCTTTGTCTCCAATT
  CAAAATTGTTTTCAGCTAATGATGTAGAAAATATATTTAGTCGCATAGTAGATATACATGAACTTAGTGT
  AAAGTTACTGGGCCATATAGAAGATACAGTAGAAATGACAGATGAAGGCAGTCCCCATCCACTAGTAGGA
  AGCTGCTTTGAAGACTTAGCAGAGGAACTGGCATTTGATCCATATGAATCGTATGCTCGAGATATTTTGC
  GACCTGGTTTTCATGATCGTTTCCTTAGTCAGTTATCAAAGCCTGGGGCAGCACTTTATTTGCAGTCAAT
  AGGCGAAGGTTTCAAAGAAGCTGTTCAATATGTTTTACCCAGGCTGCTTCTGGCCCCTGTTTACCACTGT
  CTCCATTACTTTGAACTTTTGAAGCAGTTAGAAGAAAAAAGTGAAGATCAAGAAGACAAGGAATGTTTAA
  AACAAGCAATAACAGCTTTGCTTAATGTTCAGAGTGGTATGGAAAAAATATGTTCTAAAAGTCTTGCAAA
  ACGAAGACTGAGTGAATCTGCATGTCGGTTTTATAGTCAGCAAATGAAGGGGAAACAACTAGCAATCAAG
  AAGATGAACGAGATTCAGAAGAATATTGATGGTTGGGAGGGAAAAGACATTGGACAGTGTTGTAATGAAT
  TTATAATGGAAGGAACTCTTACACGTGTAGGAGCCAAACATGAGAGACACATATTTCTCTTTGATGGCTT
  AATGATTTGCTGTAAATCAAATCATGGGCAGCCAAGACTTCCTGGTGCTAGCAATGCAGAATATCGTCTT
  AAAGAAAAGTTTTTTATGCGAAAGGTACAAATTAATGATAAAGATGACACCAATGAATACAAGCATGCTT
  TTGAAATAATTTTAAAAGATGAAAATAGTGTTATATTTTCTGCCAAGTCAGCTGAAGAGAAAAACAATTG
  GATGGCAGCATTGATATCTTTACAGTACCGGAGTACACTGGAAAGGATGCTTGATGTAACAATGCTACAG
  GAAGAGAAAGAGGAGCAGATGAGGCTGCCTAGTGCTGATGTTTATAGATTTGCAGAGCCTGACTCTGAAG
  AGAATATTATATTTGAAGAGAACATGCAGCCCAAGGCTGGAATTCCAATTATCAAAGCAGGAACTGTTAT
  TAAACTTATAGAGAGGCTTACGTACCATATGTACGCAGATCCCAATTTTGTTCGGACATTTCTTACAACA
  TACAGATCCTTTTGCAAACCTCAAGAACTACTGAGTCTTATAATAGAAAGGTTTGAAATTCCAGAGCCTG
  AGCCAACAGAAGCTGATCGCATAGCTATAGAGAATGGAGATCAACCCTTGAGTGCAGAACTGAAAAGATT
  TAGAAAAGAATATATACAGCCTGTGCAACTGCGAGTATTAAATGTATGTCGGCACTGGGTAGAGCACCAC
  TTCTATGATTTTGAAAGAGATGCATATCTTTTGCAACGAATGGAAGAATTTATTGGAACAGTAAGAGGTA
  AAGCAATGAAAAAATGGGTTGAATCCATCACTAAAATAATCCAAAGGAAAAAAATTGCAAGAGACAATGG
  ACCAGGTCATAATATTACATTTCAGAGTTCACCTCCCACAGTTGAGTGGCATATAAGCAGACCTGGGCAC
  ATAGAGACTTTTGACCTGCTCACCTTACACCCAATAGAAATTGCTCGACAACTCACTTTACTTGAATCAG
  ATCTATACCGAGCTGTACAGCCATCAGAATTAGTTGGAAGTGTGTGGACAAAAGAAGACAAAGAAATTAA
  CTCTCCTAATCTTCTGAAAATGATTCGACATACCACCAACCTCACTCTGTGGTTTGAGAAATGTATTGTA
  GAAACTGAAAATTTAGAAGAAAGAGTAGCTGTGGTGAGTCGAATTATTGAGATTCTACAAGTCTTTCAAG
  AGTTGAACAACTTTAATGGTGTCCTTGAGGTTGTCAGTGCTATGAATTCATCACCTGTTTACAGACTAGA
  CCACACATTTGAGCAAATACCAAGTCGCCAGAAGAAAATTTTAGAAGAAGCTCATGAATTGAGTGAAGAT
  CACTATAAGAAATATTTGGCAAAACTCAGGTCTATTAATCCACCATGTGTGCCTTTCTTTGGAATTTATC
  TCACTAATATCTTGAAAACAGAAGAAGGCAACCCTGAGGTCCTAAAAAGACATGGAAAAGAGCTTATAAA
  CTTTAGCAAAAGGAGGAAAGTAGCAGAAATAACAGGAGAGATCCAGCAGTACCAAAATCAGCCTTACTGT
  TTACGAGTAGAATCAGATATCAAAAGGTTCTTTGAAAACTTGAATCCGATGGGAAATAGCATGGAGAAGG
  AATTTACAGATTATCTTTTCAACAAATCCCTAGAAATAGAACCACGAAACCCTAAGCCTCTCCCAAGATT
  TCCAAAAAAATATAGCTATCCCCTAAAATCTCCTGGTGTTCGTCCATCAAACCCAAGACCAGGTACCATG
  AGGCATCCCACACCTCTGCAGCAGGAGCCAAGGAAAATTAGTTATAGTAGGATCCCTGAAAGTGAAACAG
  AAAGTACAGCATCTGCACCAAATTCTCCAAGAACACCGTTAACACCTCCGCCTGCTTCTGGTGCTTCCAG
  TACCACAGATGTTTGCAGTGTATTTGATTCCGATCATTCGAGCCCTTTTCACTCAAGCAATGATACCGTC
  TTTATCCAAGTTACTCTGCCCCATGGCCCAAGATCTGCTTCTGTATCATCTATAAGTTTAACCAAAGGCA
  CTGATGAAGTGCCTGTCCCTCCTCCTGTTCCTCCACGAAGACGACCAGAATCTGCCCCAGCAGAATCTTC
  ACCATCTAAGATTATGTCTAAGCATTTGGACAGTCCCCCAGCCATTCCTCCTAGGCAACCCACATCAAAA
  GCCTATTCACCACGATATTCAATATCAGACCGGACCTCTATCTCAGACCCTCCTGAAAGCCCTCCCTTAT
  TACCACCACGAGAACCTGTGAGGACACCTGATGTTTTCTCAAGCTCACCACTACATCTCCAACCTCCCCC
  TTTGGGCAAAAAAAGTGACCATGGCAATGCCTTCTTCCCAAACAGCCCTTCCCCCTTTACACCACCTCCT
  CCTCAAACACCTTCTCCTCACGGCACAAGAAGGCATCTGCCATCACCACCATTGACACAAGAAGTGGACC
  TTCATTCCATTGCTGGGCCGCCTGTTCCTCCACGACAAAGCACTTCTCAACATATCCCTAAACTCCCTCC
  AAAAACTTACAAAAGGGAGCACACACACCCATCCATGCACAGAGATGGACCACCACTGTTGGAGAATGCC
  CATTCTTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213531 representing NM_005633
  Red=Cloning site Green=Tags(s)
  
  MQAQQLPYEFFSEENAPKWRGLLVPALKKVQGQVHPTLESNDDALQYVEELILQLLNMLCQAQPRSASDV
  EERVQKSFPHPIDKWAIADAQSAIEKRKRRNPLSLPVEKIHPLLKEVLGYKIDHQVSVYIVAVLEYISAD
  ILKLVGNYVRNIRHYEITKQDIKVAMCADKVLMDMFHQDVEDINILSLTDEEPSTSGEQTYYDLVKAFMA
  EIRQYIRELNLIIKVFREPFVSNSKLFSANDVENIFSRIVDIHELSVKLLGHIEDTVEMTDEGSPHPLVG
  SCFEDLAEELAFDPYESYARDILRPGFHDRFLSQLSKPGAALYLQSIGEGFKEAVQYVLPRLLLAPVYHC
  LHYFELLKQLEEKSEDQEDKECLKQAITALLNVQSGMEKICSKSLAKRRLSESACRFYSQQMKGKQLAIK
  KMNEIQKNIDGWEGKDIGQCCNEFIMEGTLTRVGAKHERHIFLFDGLMICCKSNHGQPRLPGASNAEYRL
  KEKFFMRKVQINDKDDTNEYKHAFEIILKDENSVIFSAKSAEEKNNWMAALISLQYRSTLERMLDVTMLQ
  EEKEEQMRLPSADVYRFAEPDSEENIIFEENMQPKAGIPIIKAGTVIKLIERLTYHMYADPNFVRTFLTT
  YRSFCKPQELLSLIIERFEIPEPEPTEADRIAIENGDQPLSAELKRFRKEYIQPVQLRVLNVCRHWVEHH
  FYDFERDAYLLQRMEEFIGTVRGKAMKKWVESITKIIQRKKIARDNGPGHNITFQSSPPTVEWHISRPGH
  IETFDLLTLHPIEIARQLTLLESDLYRAVQPSELVGSVWTKEDKEINSPNLLKMIRHTTNLTLWFEKCIV
  ETENLEERVAVVSRIIEILQVFQELNNFNGVLEVVSAMNSSPVYRLDHTFEQIPSRQKKILEEAHELSED
  HYKKYLAKLRSINPPCVPFFGIYLTNILKTEEGNPEVLKRHGKELINFSKRRKVAEITGEIQQYQNQPYC
  LRVESDIKRFFENLNPMGNSMEKEFTDYLFNKSLEIEPRNPKPLPRFPKKYSYPLKSPGVRPSNPRPGTM
  RHPTPLQQEPRKISYSRIPESETESTASAPNSPRTPLTPPPASGASSTTDVCSVFDSDHSSPFHSSNDTV
  FIQVTLPHGPRSASVSSISLTKGTDEVPVPPPVPPRRRPESAPAESSPSKIMSKHLDSPPAIPPRQPTSK
  AYSPRYSISDRTSISDPPESPPLLPPREPVRTPDVFSSSPLHLQPPPLGKKSDHGNAFFPNSPSPFTPPP
  PQTPSPHGTRRHLPSPPLTQEVDLHSIAGPPVPPRQSTSQHIPKLPPKTYKREHTHPSMHRDGPPLLENA
  HSS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005633
• ORF Size: 3999 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005633.4
• RefSeq Size: 4002 bp
• RefSeq ORF: 4002 bp
• Locus ID: 6654
• UniProt ID: Q07889
• Cytogenetics: 2p22.1
• Domains: RhoGEF, RasGEFN, PH, RasGEF
• Protein Families: Druggable Genome
• Protein Pathways: Acute myeloid leukemia, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway
• Gene Summary: This gene encodes a protein that is a guanine nucleotide exchange factor for RAS proteins, membrane proteins that bind guanine nucleotides and participate in signal transduction pathways. GTP binding activates and GTP hydrolysis inactivates RAS proteins. The product of this gene may regulate RAS proteins by facilitating the exchange of GTP for GDP. Mutations in this gene are associated with gingival fibromatosis 1 and Noonan syndrome type 4. [provided by RefSeq, Jul 2008]