PADI1 (NM_013358) Human Tagged ORF Clone

CAT#: RG213501

PADI1 (tGFP-tagged) - Human peptidyl arginine deiminase, type I (PADI1)

Product Images

Circular map for RG213501

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PADI1
• Synonyms: HPAD10; PAD1; PDI; PDI1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213501 representing NM_013358
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCAAAGAGAGTTGTGCAGCTGTCCCTGAAGATGCCTACCCATGCCGTGTGTGTGGTGGGAGTCG
  AGGCACATGTGGACATTCACAGTGATGTGCCCAAGGGTGCCAACAGCTTCAGGGTCTCTGGAAGCTCCGG
  GGTGGAGGTCTTCATGGTCTACAACCGCACACGTGTGAAAGAGCCCATAGGCAAGGCCCGTTGGCCGCTA
  GACACTGATGCAGACATGGTCGTATCTGTGGGCACAGCCAGTAAGGAATTAAAGGACTTCAAGGTGAGGG
  TCTCCTACTTTGGGGAGCAGGAAGACCAAGCTCTGGGCCGCAGCGTGCTTTACCTCACTGGCGTCGATAT
  TTCCCTTGAGGTTGACACAGGCCGCACAGGCAAGGTGAAGAGGAGCCAAGGGGACAAGAAAACCTGGCGC
  TGGGGCCCTGAGGGCTATGGGGCTATCTTGCTGGTGAACTGTGACCGGGACAATCACAGGTCCGCAGAGC
  CTGACCTCACCCACAGCTGGCTGATGTCGCTGGCTGACCTGCAGGACATGTCCCCAATGCTGCTGAGCTG
  CAATGGCCCCGACAAGCTCTTCGACAGCCACAAGCTTGTCTTGAACGTGCCCTTTTCTGATTCCAAAAGA
  GTGAGGGTCTTCTGTGCCAGGGGTGGGAATTCTCTCTCGGACTACAAACAGGTGCTGGGGCCCCAGTGTC
  TGTCCTATGAAGTTGAGCGACAGCCAGGGGAGCAGGAGATCAAGTTCTATGTGGAGGGGCTGACCTTCCC
  CGATGCCGATTTCCTAGGGCTGGTTTCCCTCAGTGTCAGCCTGGTGGACCCGGGGACCCTGCCCGAGGTG
  ACCCTCTTCACAGACACTGTGGGCTTCCGCATGGCCCCCTGGATCATGACGCCCAACACTCAGCCTCCTG
  AGGAGCTGTATGTGTGCAGAGTGATGGACACTCATGGCTCCAATGAGAAATTCCTGGAGGACATGTCTTA
  TCTGACATTGAAAGCCAACTGCAAGCTGACCATCTGCCCTCAAGTTGAAAATCGAAATGACCGCTGGATC
  CAGGACGAGATGGAGTTTGGCTACATCGAGGCCCCTCACAAATCCTTCCCCGTGGTCTTTGACTCCCCCC
  GGAACAGGGGCCTGAAAGATTTCCCCTATAAGAGGATCCTGGGTCCTGACTTTGGATATGTTACCCGGGA
  GATCCCGCTCCCTGGTCCCTCCAGCCTTGACTCCTTCGGCAACCTGGACGTCAGCCCGCCCGTCACGGTG
  GGCGGCACGGAATACCCCCTGGGCCGGATCCTCATCGGGAGCAGCTTCCCCAAGTCCGGTGGGCGGCAGA
  TGGCCAGGGCAGTGCGGAACTTCCTGAAGGCACAGCAGGTGCAGGCACCCGTGGAGCTCTACTCGGACTG
  GCTCTCTGTGGGCCATGTGGACGAGTTTCTGACCTTTGTGCCTACCTCTGACCAAAAGGGCTTCCGGCTG
  CTCCTGGCTAGCCCCAGCGCTTGCCTCAAACTCTTCCAAGAGAAGAAAGAAGAGGGTTATGGGGAGGCAG
  CCCAGTTTGATGGGTTAAAACACCAGGCAAAAAGAAGCATTAATGAGATGCTGGCAGACAGACACCTCCA
  GAGAGACAATCTTCATGCACAGAAATGCATTGACTGGAACCGTAATGTGCTGAAGCGGGAGCTGGGCCTG
  GCAGAGAGTGACATCGTGGACATTCCCCAGCTCTTCTTCCTGAAAAACTTCTACGCGGAAGCCTTCTTCC
  CAGACATGGTTAACATGGTGGTCTTAGGCAAGTACCTGGGCATCCCCAAGCCCTACGGGCCCATCATCAA
  TGGCCGCTGCTGCCTGGAGGAGAAGGTGCAGTCCCTGCTGGAGCCTCTGGGCCTGCACTGCATCTTCATT
  GATGACTACTTGTCCTACCACGAGCTGCAGGGGGAGATCCACTGTGGCACCAACGTGCGCAGGAAGCCCT
  TTCCCTTCAAATGGTGGAACATGGTGCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213501 representing NM_013358
  Red=Cloning site Green=Tags(s)
  
  MAPKRVVQLSLKMPTHAVCVVGVEAHVDIHSDVPKGANSFRVSGSSGVEVFMVYNRTRVKEPIGKARWPL
  DTDADMVVSVGTASKELKDFKVRVSYFGEQEDQALGRSVLYLTGVDISLEVDTGRTGKVKRSQGDKKTWR
  WGPEGYGAILLVNCDRDNHRSAEPDLTHSWLMSLADLQDMSPMLLSCNGPDKLFDSHKLVLNVPFSDSKR
  VRVFCARGGNSLSDYKQVLGPQCLSYEVERQPGEQEIKFYVEGLTFPDADFLGLVSLSVSLVDPGTLPEV
  TLFTDTVGFRMAPWIMTPNTQPPEELYVCRVMDTHGSNEKFLEDMSYLTLKANCKLTICPQVENRNDRWI
  QDEMEFGYIEAPHKSFPVVFDSPRNRGLKDFPYKRILGPDFGYVTREIPLPGPSSLDSFGNLDVSPPVTV
  GGTEYPLGRILIGSSFPKSGGRQMARAVRNFLKAQQVQAPVELYSDWLSVGHVDEFLTFVPTSDQKGFRL
  LLASPSACLKLFQEKKEEGYGEAAQFDGLKHQAKRSINEMLADRHLQRDNLHAQKCIDWNRNVLKRELGL
  AESDIVDIPQLFFLKNFYAEAFFPDMVNMVVLGKYLGIPKPYGPIINGRCCLEEKVQSLLEPLGLHCIFI
  DDYLSYHELQGEIHCGTNVRRKPFPFKWWNMVP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013358
• ORF Size: 1989 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013358.3
• RefSeq Size: 2711 bp
• RefSeq ORF: 1992 bp
• Locus ID: 29943
• UniProt ID: Q9ULC6
• Cytogenetics: 1p36.13
• Gene Summary: This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type I enzyme is involved in the late stages of epidermal differentiation, where it deiminates filaggrin and keratin K1, which maintains hydration of the stratum corneum, and hence the cutaneous barrier function. This enzyme may also play a role in hair follicle formation. This gene exists in a cluster with four other paralogous genes. [provided by RefSeq, Jul 2008]