CD49b (ITGA2) (NM_002203) Human Tagged ORF Clone

CAT#: RG212617

ITGA2 (tGFP-tagged) - Human integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor) (ITGA2)

Product Images

Circular map for RG212617

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CD49b
• Synonyms: BR; CD49B; GPIa; HPA-5; VLA-2; VLAA2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212617 representing NM_002203
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCCAGAACGGACAGGGGCCGCGCCGCTGCCGCTGCTGCTGGTGTTAGCGCTCAGTCAAGGCATTT
  TAAATTGTTGTTTGGCCTACAATGTTGGTCTCCCAGAAGCAAAAATATTTTCCGGTCCTTCAAGTGAACA
  GTTTGGGTATGCAGTGCAGCAGTTTATAAATCCAAAAGGCAACTGGTTACTGGTTGGTTCACCCTGGAGT
  GGCTTTCCTGAGAACCGAATGGGAGATGTGTATAAATGTCCTGTTGACCTATCCACTGCCACATGTGAAA
  AACTAAATTTGCAAACTTCAACAAGCATTCCAAATGTTACTGAGATGAAAACCAACATGAGCCTCGGCTT
  GATCCTCACCAGGAACATGGGAACTGGAGGTTTTCTCACATGTGGTCCTCTGTGGGCACAGCAATGTGGG
  AATCAGTATTACACAACGGGTGTGTGTTCTGACATCAGTCCTGATTTTCAGCTCTCAGCCAGCTTCTCAC
  CTGCAACTCAGCCCTGCCCTTCCCTCATAGATGTTGTGGTTGTGTGTGATGAATCAAATAGTATTTATCC
  TTGGGATGCAGTAAAGAATTTTTTGGAAAAATTTGTACAAGGCCTTGATATAGGCCCCACAAAGACACAG
  GTGGGGTTAATTCAGTATGCCAATAATCCAAGAGTTGTGTTTAACTTGAACACATATAAAACCAAAGAAG
  AAATGATTGTAGCAACATCCCAGACATCCCAATATGGTGGGGACCTCACAAACACATTCGGAGCAATTCA
  ATATGCAAGAAAATATGCCTATTCAGCAGCTTCTGGTGGGCGACGAAGTGCTACGAAAGTAATGGTAGTT
  GTAACTGACGGTGAATCACATGATGGTTCAATGTTGAAAGCTGTGATTGATCAATGCAACCATGACAATA
  TACTGAGGTTTGGCATAGCAGTTCTTGGGTACTTAAACAGAAACGCCCTTGATACTAAAAATTTAATAAA
  AGAAATAAAAGCGATCGCTAGTATTCCAACAGAAAGATACTTTTTCAATGTGTCTGATGAAGCAGCTCTA
  CTAGAAAAGGCTGGGACATTAGGAGAACAAATTTTCAGCATTGAAGGTACTGTTCAAGGAGGAGACAACT
  TTCAGATGGAAATGTCACAAGTGGGATTCAGTGCAGATTACTCTTCTCAAAATGATATTCTGATGCTGGG
  TGCAGTGGGAGCTTTTGGCTGGAGTGGGACCATTGTCCAGAAGACATCTCATGGCCATTTGATCTTTCCT
  AAACAAGCCTTTGACCAAATTCTGCAGGACAGAAATCACAGTTCATATTTAGGTTACTCTGTGGCTGCAA
  TTTCTACTGGAGAAAGCACTCACTTTGTTGCTGGTGCTCCTCGGGCAAATTATACCGGCCAGATAGTGCT
  ATATAGTGTGAATGAGAATGGCAATATCACGGTTATTCAGGCTCACCGAGGTGACCAGATTGGCTCCTAT
  TTTGGTAGTGTGCTGTGTTCAGTTGATGTGGATAAAGACACCATTACAGACGTGCTCTTGGTAGGTGCAC
  CAATGTACATGAGTGACCTAAAGAAAGAGGAAGGAAGAGTCTACCTGTTTACTATCAAAAAGGGCATTTT
  GGGTCAGCACCAATTTCTTGAAGGCCCCGAGGGCATTGAAAACACTCGATTTGGTTCAGCAATTGCAGCT
  CTTTCAGACATCAACATGGATGGCTTTAATGATGTGATTGTTGGTTCACCACTAGAAAATCAGAATTCTG
  GAGCTGTATACATTTACAATGGTCATCAGGGCACTATCCGCACAAAGTATTCCCAGAAAATCTTGGGATC
  CGATGGAGCCTTTAGGAGCCATCTCCAGTACTTTGGGAGGTCCTTGGATGGCTATGGAGATTTAAATGGG
  GATTCCATCACCGATGTGTCTATTGGTGCCTTTGGACAAGTGGTTCAACTCTGGTCACAAAGTATTGCTG
  ATGTAGCTATAGAAGCTTCATTCACACCAGAAAAAATCACTTTGGTCAACAAGAATGCTCAGATAATTCT
  CAAACTCTGCTTCAGTGCAAAGTTCAGACCTACTAAGCAAAACAATCAAGTGGCCATTGTATATAACATC
  ACACTTGATGCAGATGGATTTTCATCCAGAGTAACCTCCAGGGGGTTATTTAAAGAAAACAATGAAAGGT
  GCCTGCAGAAGAATATGGTAGTAAATCAAGCACAGAGTTGCCCCGAGCACATCATTTATATACAGGAGCC
  CTCTGATGTTGTCAACTCTTTGGATTTGCGTGTGGACATCAGTCTGGAAAACCCTGGCACTAGCCCTGCC
  CTTGAAGCCTATTCTGAGACTGCCAAGGTCTTCAGTATTCCTTTCCACAAAGACTGTGGTGAGGATGGAC
  TTTGCATTTCTGATCTAGTCCTAGATGTCCGACAAATACCAGCTGCTCAAGAACAACCCTTTATTGTCAG
  CAACCAAAACAAAAGGTTAACATTTTCAGTAACACTGAAAAATAAAAGGGAAAGTGCATACAACACTGGA
  ATTGTTGTTGATTTTTCAGAAAACTTGTTTTTTGCATCATTCTCCCTACCGGTTGATGGGACAGAAGTAA
  CATGCCAGGTGGCTGCATCTCAGAAGTCTGTTGCCTGCGATGTAGGCTACCCTGCTTTAAAGAGAGAACA
  ACAGGTGACTTTTACTATTAACTTTGACTTCAATCTTCAAAACCTTCAGAATCAGGCGTCTCTCAGTTTC
  CAAGCCTTAAGTGAAAGCCAAGAAGAAAACAAGGCTGATAATTTGGTCAACCTCAAAATTCCTCTCCTGT
  ATGATGCTGAAATTCACTTAACAAGATCTACCAACATAAATTTTTATGAAATCTCTTCGGATGGGAATGT
  TCCTTCAATCGTGCACAGTTTTGAAGATGTTGGTCCAAAATTCATCTTCTCCCTGAAGGTAACAACAGGA
  AGTGTTCCAGTAAGCATGGCAACTGTAATCATCCACATCCCTCAGTATACCAAAGAAAAGAACCCACTGA
  TGTACCTAACTGGGGTGCAAACAGACAAGGCTGGTGACATCAGTTGTAATGCAGATATCAATCCACTGAA
  AATAGGACAAACATCTTCTTCTGTATCTTTCAAAAGTGAAAATTTCAGGCACACCAAAGAATTGAACTGC
  AGAACTGCTTCCTGTAGTAATGTTACCTGCTGGTTGAAAGACGTTCACATGAAAGGAGAATACTTTGTTA
  ATGTGACTACCAGAATTTGGAACGGGACTTTCGCATCATCAACGTTCCAGACAGTACAGCTAACGGCAGC
  TGCAGAAATCAACACCTATAACCCTGAGATATATGTGATTGAAGATAACACTGTTACGATTCCCCTGATG
  ATAATGAAACCTGATGAGAAAGCCGAAGTACCAACAGGAGTTATAATAGGAAGTATAATTGCTGGAATCC
  TTTTGCTGTTAGCTCTGGTTGCAATTTTATGGAAGCTCGGCTTCTTCAAAAGAAAATATGAAAAGATGAC
  CAAAAATCCAGATGAGATTGATGAGACCACAGAGCTCAGTAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212617 representing NM_002203
  Red=Cloning site Green=Tags(s)
  
  MGPERTGAAPLPLLLVLALSQGILNCCLAYNVGLPEAKIFSGPSSEQFGYAVQQFINPKGNWLLVGSPWS
  GFPENRMGDVYKCPVDLSTATCEKLNLQTSTSIPNVTEMKTNMSLGLILTRNMGTGGFLTCGPLWAQQCG
  NQYYTTGVCSDISPDFQLSASFSPATQPCPSLIDVVVVCDESNSIYPWDAVKNFLEKFVQGLDIGPTKTQ
  VGLIQYANNPRVVFNLNTYKTKEEMIVATSQTSQYGGDLTNTFGAIQYARKYAYSAASGGRRSATKVMVV
  VTDGESHDGSMLKAVIDQCNHDNILRFGIAVLGYLNRNALDTKNLIKEIKAIASIPTERYFFNVSDEAAL
  LEKAGTLGEQIFSIEGTVQGGDNFQMEMSQVGFSADYSSQNDILMLGAVGAFGWSGTIVQKTSHGHLIFP
  KQAFDQILQDRNHSSYLGYSVAAISTGESTHFVAGAPRANYTGQIVLYSVNENGNITVIQAHRGDQIGSY
  FGSVLCSVDVDKDTITDVLLVGAPMYMSDLKKEEGRVYLFTIKKGILGQHQFLEGPEGIENTRFGSAIAA
  LSDINMDGFNDVIVGSPLENQNSGAVYIYNGHQGTIRTKYSQKILGSDGAFRSHLQYFGRSLDGYGDLNG
  DSITDVSIGAFGQVVQLWSQSIADVAIEASFTPEKITLVNKNAQIILKLCFSAKFRPTKQNNQVAIVYNI
  TLDADGFSSRVTSRGLFKENNERCLQKNMVVNQAQSCPEHIIYIQEPSDVVNSLDLRVDISLENPGTSPA
  LEAYSETAKVFSIPFHKDCGEDGLCISDLVLDVRQIPAAQEQPFIVSNQNKRLTFSVTLKNKRESAYNTG
  IVVDFSENLFFASFSLPVDGTEVTCQVAASQKSVACDVGYPALKREQQVTFTINFDFNLQNLQNQASLSF
  QALSESQEENKADNLVNLKIPLLYDAEIHLTRSTNINFYEISSDGNVPSIVHSFEDVGPKFIFSLKVTTG
  SVPVSMATVIIHIPQYTKEKNPLMYLTGVQTDKAGDISCNADINPLKIGQTSSSVSFKSENFRHTKELNC
  RTASCSNVTCWLKDVHMKGEYFVNVTTRIWNGTFASSTFQTVQLTAAAEINTYNPEIYVIEDNTVTIPLM
  IMKPDEKAEVPTGVIIGSIIAGILLLLALVAILWKLGFFKRKYEKMTKNPDEIDETTELSS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002203
• ORF Size: 3543 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002203.2, NP_002194.1
• RefSeq Size: 5361 bp
• RefSeq ORF: 3546 bp
• Locus ID: 3673
• UniProt ID: P17301
• Cytogenetics: 5q11.2
• Domains: FG-GAP, VWA
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), Pathways in cancer, Regulation of actin cytoskeleton, Small cell lung cancer
• Gene Summary: This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]