GABRA2 (NM_000807) Human Tagged ORF Clone

CAT#: RG212430

GABRA2 (tGFP-tagged) - Human gamma-aminobutyric acid (GABA) A receptor, alpha 2 (GABRA2), transcript variant 1

Product Images

Circular map for RG212430

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GABRA2
• Synonyms: DEE78; EIEE78
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212430 representing NM_000807
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGACAAAATTGAACATCTACAACATCGAGTTCCTGCTTTTTGTTTTCTTGGTGTGGGACCCTGCCA
  GGTTGGTGCTGGCTAACATCCAAGAAGATGAGGCTAAAAATAACATTACCATCTTTACGAGAATTCTTGA
  CAGACTTCTGGATGGTTACGATAATCGGCTTAGACCAGGACTGGGAGACAGTATTACTGAAGTCTTCACT
  AACATCTACGTGACCAGTTTTGGCCCTGTCTCAGATACAGATATGGAATATACAATTGATGTTTTCTTTC
  GACAAAAATGGAAAGATGAACGTTTAAAATTTAAAGGTCCTATGAATATCCTTCGACTAAACAATTTAAT
  GGCTAGCAAAATCTGGACTCCAGATACCTTTTTTCACAATGGGAAGAAATCAGTAGCTCATAATATGACA
  ATGCCAAATAAGTTGCTTCGAATTCAGGATGATGGGACTCTGCTGTATACCATGAGGCTTACAGTTCAAG
  CTGAATGCCCAATGCACTTGGAGGATTTCCCAATGGATGCTCATTCATGTCCTCTGAAATTTGGCAGCTA
  TGCATATACAACTTCAGAGGTCACTTATATTTGGACTTACAATGCATCTGATTCAGTACAGGTTGCTCCT
  GATGGCTCTAGGTTAAATCAATATGACCTGCTGGGCCAATCAATCGGAAAGGAGACAATTAAATCCAGTA
  CAGGTGAATATACTGTAATGACAGCTCATTTCCACCTGAAAAGAAAAATTGGGTATTTTGTGATTCAAAC
  CTATCTGCCTTGCATCATGACTGTCATTCTCTCCCAAGTTTCATTCTGGCTTAACAGAGAATCTGTGCCT
  GCAAGAACTGTGTTTGGAGTAACAACTGTCCTAACAATGACAACTCTAAGCATCAGTGCTCGGAATTCTC
  TCCCCAAAGTGGCTTATGCAACTGCCATGGACTGGTTTATTGCTGTTTGTTATGCATTTGTGTTCTCTGC
  CCTAATTGAATTTGCAACTGTTAATTACTTCACCAAAAGAGGATGGACTTGGGATGGGAAGAGTGTAGTA
  AATGACAAGAAAAAAGAAAAGGCTTCCGTTATGATACAGAACAACGCTTATGCAGTGGCTGTTGCCAATT
  ATGCCCCGAATCTTTCAAAAGATCCAGTTCTCTCCACCATCTCCAAGAGTGCAACCACGCCAGAACCCAA
  CAAGAAGCCAGAAAACAAGCCAGCTGAAGCAAAGAAAACTTTCAACAGTGTTAGCAAAATTGACAGAATG
  TCCAGAATAGTTTTTCCAGTTTTGTTTGGTACCTTTAATTTAGTTTACTGGGCTACATATTTAAACAGAG
  AACCTGTATTAGGGGTCAGTCCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212430 representing NM_000807
  Red=Cloning site Green=Tags(s)
  
  MKTKLNIYNIEFLLFVFLVWDPARLVLANIQEDEAKNNITIFTRILDRLLDGYDNRLRPGLGDSITEVFT
  NIYVTSFGPVSDTDMEYTIDVFFRQKWKDERLKFKGPMNILRLNNLMASKIWTPDTFFHNGKKSVAHNMT
  MPNKLLRIQDDGTLLYTMRLTVQAECPMHLEDFPMDAHSCPLKFGSYAYTTSEVTYIWTYNASDSVQVAP
  DGSRLNQYDLLGQSIGKETIKSSTGEYTVMTAHFHLKRKIGYFVIQTYLPCIMTVILSQVSFWLNRESVP
  ARTVFGVTTVLTMTTLSISARNSLPKVAYATAMDWFIAVCYAFVFSALIEFATVNYFTKRGWTWDGKSVV
  NDKKKEKASVMIQNNAYAVAVANYAPNLSKDPVLSTISKSATTPEPNKKPENKPAEAKKTFNSVSKIDRM
  SRIVFPVLFGTFNLVYWATYLNREPVLGVSP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000807
• ORF Size: 1353 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000807.1, NP_000798.1
• RefSeq Size: 2189 bp
• RefSeq ORF: 1356 bp
• Locus ID: 2555
• UniProt ID: P47869
• Cytogenetics: 4p12
• Domains: Neur_chan_memb, Neur_chan_LBD
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]