CRAT (NM_000755) Human Tagged ORF Clone

CAT#: RG212196

CRAT (tGFP-tagged) - Human carnitine O-acetyltransferase (CRAT), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Images

Circular map for RG212196

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CRAT
• Synonyms: CAT; CAT1; NBIA8
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212196 representing NM_000755
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTAGCCTTCGCTGCCAGGACCGTGGTGAAGCCTCTGGGCTTCCTGAAGCCCTTCTCCTTGATGAAGG
  CTTCCAGCCGCTTCAAGGCACACCAGGATGCACTGCCACGGCTGCCCGTGCCCCCTCTCCAGCAGTCCCT
  GGACCACTACCTGAAGGCGCTGCAGCCCATCGTGAGTGAGGAGGAGTGGGCCCACACCAAGCAGCTGGTG
  GATGAGTTTCAGGCCTCAGGAGGTGTAGGGGAGCGCCTGCAGAAGGGGCTGGAGCGTCGGGCCAGGAAGA
  CGGAGAACTGGCTGTCTGAGTGGTGGCTCAAGACCGCCTACCTCCAGTACCGCCAGCCTGTGGTCATCTA
  CTCGAGCCCAGGCGTGATGCTACCCAAGCAGGACTTCGTGGACCTGCAGGGTCAGCTCCGATTTGCTGCC
  AAACTCATTGAGGGTGTGTTGGATTTCAAGGTCATGATTGACAACGAGACCCTGCCCGTGGAGTACCTGG
  GGGGGAAGCCACTGTGCATGAACCAGTACTATCAGATCTTGTCCTCCTGCCGAGTGCCGGGCCCCAAGCA
  GGACACAGTCAGCAACTTCAGCAAGACCAAGAAGCCTCCCACGCACATCACCGTGGTACACAACTACCAG
  TTTTTTGAGCTGGATGTGTACCACAGTGACGGGACACCCCTCACTGCGGATCAGATCTTTGTGCAGCTGG
  AGAAGATCTGGAACTCATCCCTACAGACCAACAAGGAGCCTGTGGGCATCCTCACCTCCAACCACCGCAA
  CTCCTGGGCCAAGGCATACAACACCCTCATCAAAGACAAGGTGAACCGGGATTCCGTGCGCTCCATCCAG
  AAGAGCATCTTCACCGTGTGCCTAGATGCAACCATGCCCAGGGTCTCAGAAGACGTGTACCGCAGCCACG
  TGGCAGGCCAGATGCTGCATGGGGGCGGCAGCAGGCTCAACAGCGGCAACCGCTGGTTCGACAAGACGCT
  GCAGTTCATCGTGGCAAAAGATGGCTCCTGTGGGCTTGTGTACGAGCATGCTGCAGCGGAGGGGCCCCCT
  ATTGTCACCCTTCTGGACTATGTCATCGAGTACACGAAGAAACCCGAGCTTGTGCGGTCTCCCATGGTGC
  CCCTGCCCATGCCCAAGAAGCTGCGGTTCAACATCACCCCCGAGATCAAGAGCGACATCGAGAAGGCCAA
  GCAGAACCTCAGCATCATGATCCAGGACCTGGATATCACCGTGATGGTGTTCCACCATTTTGGAAAAGAC
  TTCCCCAAGTCGGAGAAGCTAAGCCCAGATGCCTTCATCCAGATGGCTTTGCAGCTGGCCTACTACAGGA
  TCTACGGACAGGCATGTGCCACCTATGAAAGTGCCTCCCTGCGCATGTTTCACCTGGGCCGCACCGACAC
  CATCCGCTCGGCTTCCATGGACTCACTCACCTTTGTCAAGGCCATGGATGACTCCAGCGTCACGGAGCAC
  CAGAAGGTGGAGCTGCTGCGGAAGGCCGTGCAGGCCCACCGAGGCTACACCGACCGGGCCATCCGCGGGG
  AGGCCTTTGATCGACACCTGCTGGGCCTGAAGCTGCAGGCCATCGAGGACCTGGTGAGCATGCCCGACAT
  CTTCATGGACACCTCCTACGCCATCGCCATGCACTTCCACCTCTCCACCAGCCAGGTCCCTGCCAAGACA
  GACTGTGTCATGTTCTTCGGGCCCGTGGTCCCCGACGGCTACGGTGTCTGCTATAACCCCATGGAGGCCC
  ACATCAACTTCTCCCTGTCGGCCTACAACAGCTGCGCGGAGACCAACGCCGCCCGCCTGGCGCATTACCT
  GGAGAAGGCGCTCCTGGACATGCGTGCCCTGCTGCAGAGCCACCCCCGGGCCAAGCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212196 representing NM_000755
  Red=Cloning site Green=Tags(s)
  
  MLAFAARTVVKPLGFLKPFSLMKASSRFKAHQDALPRLPVPPLQQSLDHYLKALQPIVSEEEWAHTKQLV
  DEFQASGGVGERLQKGLERRARKTENWLSEWWLKTAYLQYRQPVVIYSSPGVMLPKQDFVDLQGQLRFAA
  KLIEGVLDFKVMIDNETLPVEYLGGKPLCMNQYYQILSSCRVPGPKQDTVSNFSKTKKPPTHITVVHNYQ
  FFELDVYHSDGTPLTADQIFVQLEKIWNSSLQTNKEPVGILTSNHRNSWAKAYNTLIKDKVNRDSVRSIQ
  KSIFTVCLDATMPRVSEDVYRSHVAGQMLHGGGSRLNSGNRWFDKTLQFIVAKDGSCGLVYEHAAAEGPP
  IVTLLDYVIEYTKKPELVRSPMVPLPMPKKLRFNITPEIKSDIEKAKQNLSIMIQDLDITVMVFHHFGKD
  FPKSEKLSPDAFIQMALQLAYYRIYGQACATYESASLRMFHLGRTDTIRSASMDSLTFVKAMDDSSVTEH
  QKVELLRKAVQAHRGYTDRAIRGEAFDRHLLGLKLQAIEDLVSMPDIFMDTSYAIAMHFHLSTSQVPAKT
  DCVMFFGPVVPDGYGVCYNPMEAHINFSLSAYNSCAETNAARLAHYLEKALLDMRALLQSHPRAKL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000755
• ORF Size: 1878 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000755.5
• RefSeq Size: 2804 bp
• RefSeq ORF: 1881 bp
• Locus ID: 1384
• Cytogenetics: 9q34.11
• Domains: Carn_acyltransf
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes carnitine O-acetyltransferase, a member of the carnitine acyltransferase family and a key metabolic pathway enzyme which plays an important role in energy homeostasis and fat metabolism. This enzyme catalyzes the reversible transfer of acyl groups from an acyl-CoA thioester to carnitine and regulates the ratio of acyl-CoA/CoA. It is found in both the mitochondria and the peroxisome. Alternative splicing results in transcript variants encoding different isoforms that may localize to different subcellular compartments. [provided by RefSeq, Oct 2016]