FAM190B (CCSER2) (NM_018999) Human Tagged ORF Clone

CAT#: RG212137

CCSER2 (tGFP-tagged) - Human family with sequence similarity 190, member B (FAM190B)

Product Images

Circular map for RG212137

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: FAM190B
• Synonyms: bA486O22.1; FAM190B; Gcap14; KIAA1128
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212137 representing NM_018999
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAAAAAACACAAATCAAGACATTTTTGGGTTCCAAGTTGCCAAAGTATGGAACAAAATCTGTAA
  GAAGTACATTGCAGCCAATGCCAAATGGGACACCTGTTAATTTATTAGGAACTTCCAAGAATAGTAATGT
  CAAAAGTTACATCAAAAATAATGGCTCTGATTGTCCATCATCTCATTCATTTAATTGGAGGAAAGCAAAT
  AAATATCAGCTTTGTGCACAAGGTGTCGAAGAGCCTAACAATACTCAAAATTCACATGATAAAATAATTG
  ATCCTGAAAAACGTGTTCCTACTCAAGGAATGTTTGATAAAAATGGGATAAAGGGAGGTTTGAAAAGTGT
  TTCTTTATTCACATCAAAGTTAGCAAAGCCATCCACTATGTTTGTGTCATCTACAGAGGAGTTAAACCAA
  AAGTCTTTTTCTGGACCATCTAATTTGGGTAAATTCACCAAAGGCACATTATTAGGAAGGACTTCATATT
  CTTCGATCAATACTCCAAAATCACAGTTGAATGGATTTTATGGAAACCGATCAGCTGGTAGCATGCAAAG
  GCCTAGAGCGAACTCCTGTGCCACCAGAAGCAGTTCTGGAGAAAGCTTAGCTCAATCCCCAGACAGTAGT
  AAATCTATTAATTGTGAAAAAATGGTAAGGTCACAAAGTTTTTCACATTCCATTCAGAATTCATTCCTTC
  CACCTTCATCTATAACCAGATCACATTCCTTTAATAGAGCTGTGGATCTTACAAAGCCTTATCAGAACCA
  ACAGCTATCCATTAGAGTGCCTCTACGGTCAAGTATGCTAACAAGAAATTCCCGGCAGCCAGAAGTACTC
  AATGGGAATGAACATTTGGGGTATGGATTTAATAGGCCTTATGCTGCTGGTGGAAAGAAGTTGGCTTTAC
  CAAATGGCCCAGGTGTAACTTCTACTTTAGGTTATAGAATGGTTCATCCCTCTCTACTGAAATCTAGCCG
  ATCTCCATTTTCTGGGACTATGACAGTTGATGGAAATAAAAATTCACCTGCTGACACATGTGTAGAGGAA
  GATGCTACAGTTTTGGCTAAGGACAGAGCTGCTAATAAGGACCAAGAACTGATTGAAAATGAAAGTTATA
  GAACAAAAAACAACCAGACCATGAAACATGATGCTAAAATGAGATACCTGAGTGATGATGTGGATGACAT
  TTCCTTGTCGTCTTTGTCATCTTCTGATAAGAATGATTTAAGTGAAGACTTTAGTGATGATTTTATAGAT
  ATAGAAGACTCCAACAGAACTAGAATAACTCCAGAGGAAATGTCTCTCAAAGAAGAGAAACATGAAAATG
  GGCCACCACAGGATATGTTTGATTCCCCCAAGGAAAATGAAAAAGCCTTCAGTAAAACTGATGAATGGAT
  AGATATAAGTGTCTCTGACAGGAGTGAATGTACAAAACATACTTCTGGGAATAATTTGGTTTCACCAGAT
  ACAGACTACAGAGCTGGTTCTTCGTTTGAACTCTCTCCATCTGATAGCTCTGATGGAACATACATGTGGG
  ATGAAGAAGGCTTGGAACCCATTGGAAATGTCCATCCAGTTGGGAGCTATGAGTCCTCTGAAATGAACAG
  CATAGATATTTTGAATAATCTTGAATCATGTGACCTTGAGGATGATGATCTTATGCTTGATGTGGATCTG
  CCTGAGGATGCACCTCTTGAAAATGTGGAGTGTGACAATATGAACCGCTTTGACCGACCAGACAGAAATG
  TTCGGCAGCCTCAGGAAGGTTTTTGGAAAAGGCCACCCCAGAGGTGGAGTGGACAGGAGCATTACCACCT
  CAGCCACCCTGACCACTATCATCACCATGGAAAAAGTGACTTGAGCAGAGGCTCTCCCTATAGAGAATCT
  CCTTTGGGTCATTTTGAAAGCTATGGAGGGATGCCCTTTTTCCAGGCTCAGAAGATGTTTGTTGATGTAC
  CAGAAAATACAGTGATACTGGATGAGATGACCCTTCGGCACATGGTTCAGGATTGCACTGCTGTAAAAAC
  TCAGTTACTCAAACTGAAACGTCTCCTGCATCAGCATGATGGAAGTGGTTCATTGCATGATATTCAACTG
  TCATTGCCATCCAGTCCAGAACCAGAAGATGGTGATAAAGTATATAAGAATGAAGATTTATTAAATGAAA
  TAAAACAACTTAAAGACGAAATAAAGAAAAAAGATGAAAAGATCCAACTATTAGAACTTCAGCTTGCAAC
  TCAGCATATCTGCCACCAAAAATGTAAAGAGGAAAAATGCACTTATGCTGATAAATATACCCAAACACCC
  TGGAGACGAATTCCTGGTGGGTATTCTGCTCCCTCCTTCTCTCCTTGGCAGGGCTCCTTCCAGGGGATCC
  CACGGACTGTTCCACCGCACCGCAGACAGACCTCAAGTACTACAGCCTTCCAGCAGCCTTCCCAGACCCA
  CAGATCACACCCAGGGAAAACTAATAAAGCCACAACGTATCGAGGCCCGCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212137 representing NM_018999
  Red=Cloning site Green=Tags(s)
  
  MEEKTQIKTFLGSKLPKYGTKSVRSTLQPMPNGTPVNLLGTSKNSNVKSYIKNNGSDCPSSHSFNWRKAN
  KYQLCAQGVEEPNNTQNSHDKIIDPEKRVPTQGMFDKNGIKGGLKSVSLFTSKLAKPSTMFVSSTEELNQ
  KSFSGPSNLGKFTKGTLLGRTSYSSINTPKSQLNGFYGNRSAGSMQRPRANSCATRSSSGESLAQSPDSS
  KSINCEKMVRSQSFSHSIQNSFLPPSSITRSHSFNRAVDLTKPYQNQQLSIRVPLRSSMLTRNSRQPEVL
  NGNEHLGYGFNRPYAAGGKKLALPNGPGVTSTLGYRMVHPSLLKSSRSPFSGTMTVDGNKNSPADTCVEE
  DATVLAKDRAANKDQELIENESYRTKNNQTMKHDAKMRYLSDDVDDISLSSLSSSDKNDLSEDFSDDFID
  IEDSNRTRITPEEMSLKEEKHENGPPQDMFDSPKENEKAFSKTDEWIDISVSDRSECTKHTSGNNLVSPD
  TDYRAGSSFELSPSDSSDGTYMWDEEGLEPIGNVHPVGSYESSEMNSIDILNNLESCDLEDDDLMLDVDL
  PEDAPLENVECDNMNRFDRPDRNVRQPQEGFWKRPPQRWSGQEHYHLSHPDHYHHHGKSDLSRGSPYRES
  PLGHFESYGGMPFFQAQKMFVDVPENTVILDEMTLRHMVQDCTAVKTQLLKLKRLLHQHDGSGSLHDIQL
  SLPSSPEPEDGDKVYKNEDLLNEIKQLKDEIKKKDEKIQLLELQLATQHICHQKCKEEKCTYADKYTQTP
  WRRIPGGYSAPSFSPWQGSFQGIPRTVPPHRRQTSSTTAFQQPSQTHRSHPGKTNKATTYRGPQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018999
• ORF Size: 2502 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018999.4
• RefSeq Size: 7676 bp
• RefSeq ORF: 2505 bp
• Locus ID: 54462
• UniProt ID: Q9H7U1
• Cytogenetics: 10q23.1
• Protein Families: Druggable Genome
• Gene Summary: Microtubule-binding protein which might play a role in microtubule bundling.[UniProtKB/Swiss-Prot Function]