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Nav1.5 (SCN5A) (NM_001099405) Human Tagged ORF Clone
CAT#: RG212010
- TrueORF®
SCN5A (tGFP-tagged) - Human sodium channel, voltage-gated, type V, alpha subunit (SCN5A), transcript variant 4
ORF Plasmid: DDK
"NM_001099405" in other vectors (2)
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Other products for "Nav1.5"
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Symbol | Nav1.5 |
| Synonyms | CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1; ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG212010 representing NM_001099405
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAACTTCCTATTACCTCGGGGCACCAGCAGCTTCCGCAGGTTCACACGGGAGTCCCTGGCAGCCA TCGAGAAGCGCATGGCAGAGAAGCAAGCCCGCGGCTCAACCACCTTGCAGGAGAGCCGAGAGGGGCTGCC CGAGGAGGAGGCTCCCCGGCCCCAGCTGGACCTGCAGGCCTCCAAAAAGCTGCCAGATCTCTATGGCAAT CCACCCCAAGAGCTCATCGGAGAGCCCCTGGAGGACCTGGACCCCTTCTATAGCACCCAAAAGACTTTCA TCGTACTGAATAAAGGCAAGACCATCTTCCGGTTCAGTGCCACCAACGCCTTGTATGTCCTCAGTCCCTT CCACCCCATCCGGAGAGCGGCTGTGAAGATTCTGGTTCACTCGCTCTTCAACATGCTCATCATGTGCACC ATCCTCACCAACTGCGTGTTCATGGCCCAGCACGACCCTCCACCCTGGACCAAGTATGTCGAGTACACCT TCACCGCCATTTACACCTTTGAGTCTCTGGTCAAGATTCTGGCTCGAGGCTTCTGCCTGCACGCGTTCAC TTTCCTTCGGGACCCATGGAACTGGCTGGACTTTAGTGTGATTATCATGGCGTATGTATCAGAAAATATA AAACTAGGCAATTTGTCGGCTCTTCGAACTTTCAGAGTCCTGAGAGCTCTAAAAACTATTTCAGTTATCC CAGGGCTGAAGACCATCGTGGGGGCCCTGATCCAGTCTGTGAAGAAGCTGGCTGATGTGATGGTCCTCAC AGTCTTCTGCCTCAGCGTCTTTGCCCTCATCGGCCTGCAGCTCTTCATGGGCAACCTAAGGCACAAGTGC GTGCGCAACTTCACAGCGCTCAACGGCACCAACGGCTCCGTGGAGGCCGACGGCTTGGTCTGGGAATCCC TGGACCTTTACCTCAGTGATCCAGAAAATTACCTGCTCAAGAACGGCACCTCTGATGTGTTACTGTGTGG GAACAGCTCTGACGCTGGGACATGTCCGGAGGGCTACCGGTGCCTAAAGGCAGGCGAGAACCCCGACCAC GGCTACACCAGCTTCGATTCCTTTGCCTGGGCCTTTCTTGCACTCTTCCGCCTGATGACGCAGGACTGCT GGGAGCGCCTCTATCAGCAGACCCTCAGGTCCGCAGGGAAGATCTACATGATCTTCTTCATGCTTGTCAT CTTCCTGGGGTCCTTCTACCTGGTGAACCTGATCCTGGCCGTGGTCGCAATGGCCTATGAGGAGCAAAAC CAAGCCACCATCGCTGAGACCGAGGAGAAGGAAAAGCGCTTCCAGGAGGCCATGGAAATGCTCAAGAAAG AACACGAGGCCCTCACCATCAGGGGTGTGGATACCGTGTCCCGTAGCTCCTTGGAGATGTCCCCTTTGGC CCCAGTAAACAGCCATGAGAGAAGAAGCAAGAGGAGAAAACGGATGTCTTCAGGAACTGAGGAGTGTGGG GAGGACAGGCTCCCCAAGTCTGACTCAGAAGATGGTCCCAGAGCAATGAATCATCTCAGCCTCACCCGTG GCCTCAGCAGGACTTCTATGAAGCCACGTTCCAGCCGCGGGAGCATTTTCACCTTTCGCAGGCGAGACCT GGGTTCTGAAGCAGATTTTGCAGATGATGAAAACAGCACAGCGGGGGAGAGCGAGAGCCACCACACATCA CTGCTGGTGCCCTGGCCCCTGCGCCGGACCAGTGCCCAGGGACAGCCCAGTCCCGGAACCTCGGCTCCTG GCCACGCCCTCCATGGCAAAAAGAACAGCACTGTGGACTGCAATGGGGTGGTCTCATTACTGGGGGCAGG CGACCCAGAGGCCACATCCCCAGGAAGCCACCTCCTCCGCCCTGTGATGCTAGAGCACCCGCCAGACACG ACCACGCCATCGGAGGAGCCAGGCGGGCCCCAGATGCTGACCTCCCAGGCTCCGTGTGTAGATGGCTTCG AGGAGCCAGGAGCACGGCAGCGGGCCCTCAGCGCAGTCAGCGTCCTCACCAGCGCACTGGAAGAGTTAGA GGAGTCTCGCCACAAGTGTCCACCATGCTGGAACCGTCTCGCCCAGCGCTACCTGATCTGGGAGTGCTGC CCGCTGTGGATGTCCATCAAGCAGGGAGTGAAGTTGGTGGTCATGGACCCGTTTACTGACCTCACCATCA CTATGTGCATCGTACTCAACACACTCTTCATGGCGCTGGAGCACTACAACATGACAAGTGAATTCGAGGA GATGCTGCAGGTCGGAAACCTGGTCTTCACAGGGATTTTCACAGCAGAGATGACCTTCAAGATCATTGCC CTCGACCCCTACTACTACTTCCAACAGGGCTGGAACATCTTCGACAGCATCATCGTCATCCTTAGCCTCA TGGAGCTGGGCCTGTCCCGCATGAGCAACTTGTCGGTGCTGCGCTCCTTCCGCCTGCTGCGGGTCTTCAA GCTGGCCAAATCATGGCCCACCCTGAACACACTCATCAAGATCATCGGGAACTCAGTGGGGGCACTGGGG AACCTGACACTGGTGCTAGCCATCATCGTGTTCATCTTTGCTGTGGTGGGCATGCAGCTCTTTGGCAAGA ACTACTCGGAGCTGAGGGACAGCGACTCAGGCCTGCTGCCTCGCTGGCACATGATGGACTTCTTTCATGC CTTCCTCATCATCTTCCGCATCCTCTGTGGAGAGTGGATCGAGACCATGTGGGACTGCATGGAGGTGTCG GGGCAGTCATTATGCCTGCTGGTCTTCTTGCTTGTTATGGTCATTGGCAACCTTGTGGTCCTGAATCTCT TCCTGGCCTTGCTGCTCAGCTCCTTCAGTGCAGACAACCTCACAGCCCCTGATGAGGACAGAGAGATGAA CAACCTCCAGCTGGCCCTGGCCCGCATCCAGAGGGGCCTGCGCTTTGTCAAGCGGACCACCTGGGATTTC TGCTGTGGTCTCCTGCGGCAGCGGCCTCAGAAGCCCGCAGCCCTTGCCGCCCAGGGCCAGCTGCCCAGCT GCATTGCCACCCCCTACTCCCCGCCACCCCCAGAGACGGAGAAGGTGCCTCCCACCCGCAAGGAAACACG GTTTGAGGAAGGCGAGCAACCAGGCCAGGGCACCCCCGGGGATCCAGAGCCCGTGTGTGTGCCCATCGCT GTGGCCGAGTCAGACACAGATGACCAAGAAGAAGATGAGGAGAACAGCCTGGGCACGGAGGAGGAGTCCA GCAAGCAGCAGGAATCCCAGCCTGTGTCCGGTGGCCCAGAGGCCCCTCCGGATTCCAGGACCTGGAGCCA GGTGTCAGCGACTGCCTCCTCTGAGGCCGAGGCCAGTGCATCTCAGGCCGACTGGCGGCAGCAGTGGAAA GCGGAACCCCAGGCCCCAGGGTGCGGTGAGACCCCAGAGGACAGTTGCTCCGAGGGCAGCACAGCAGACA TGACCAACACCGCTGAGCTCCTGGAGCAGATCCCTGACCTCGGCCAGGATGTCAAGGACCCAGAGGACTG CTTCACTGAAGGCTGTGTCCGGCGCTGTCCCTGCTGTGCGGTGGACACCACACAGGCCCCAGGGAAGGTC TGGTGGCGGTTGCGCAAGACCTGCTACCACATCGTGGAGCACAGCTGGTTCGAGACATTCATCATCTTCA TGATCCTACTCAGCAGTGGAGCGCTGGCCTTCGAGGACATCTACCTAGAGGAGCGGAAGACCATCAAGGT TCTGCTTGAGTATGCCGACAAGATGTTCACATATGTCTTCGTGCTGGAGATGCTGCTCAAGTGGGTGGCC TACGGCTTCAAGAAGTACTTCACCAATGCCTGGTGCTGGCTCGACTTCCTCATCGTAGACGTCTCTCTGG TCAGCCTGGTGGCCAACACCCTGGGCTTTGCCGAGATGGGCCCCATCAAGTCACTGCGGACGCTGCGTGC ACTCCGTCCTCTGAGAGCTCTGTCACGATTTGAGGGCATGAGGGTGGTGGTCAATGCCCTGGTGGGCGCC ATCCCGTCCATCATGAACGTCCTCCTCGTCTGCCTCATCTTCTGGCTCATCTTCAGCATCATGGGCGTGA ACCTCTTTGCGGGGAAGTTTGGGAGGTGCATCAACCAGACAGAGGGAGACTTGCCTTTGAACTACACCAT CGTGAACAACAAGAGCCAGTGTGAGTCCTTGAACTTGACCGGAGAATTGTACTGGACCAAGGTGAAAGTC AACTTTGACAACGTGGGGGCCGGGTACCTGGCCCTTCTGCAGGTGTATGAAGAGCAGCCTCAGTGGGAAT ACAACCTCTACATGTACATCTATTTTGTCATTTTCATCATCTTTGGGTCTTTCTTCACCCTGAACCTCTT TATTGGTGTCATCATTGACAACTTCAACCAACAGAAGAAAAAGTTAGGGGGCCAGGACATCTTCATGACA GAGGAGCAGAAGAAGTACTACAATGCCATGAAGAAGCTGGGCTCCAAGAAGCCCCAGAAGCCCATCCCAC GGCCCCTGAACAAGTACCAGGGCTTCATATTCGACATTGTGACCAAGCAGGCCTTTGACGTCACCATCAT GTTTCTGATCTGCTTGAATATGGTGACCATGATGGTGGAGACAGATGACCAAAGTCCTGAGAAAATCAAC ATCTTGGCCAAGATCAACCTGCTCTTTGTGGCCATCTTCACAGGCGAGTGTATTGTCAAGCTGGCTGCCC TGCGCCACTACTACTTCACCAACAGCTGGAATATCTTCGACTTCGTGGTTGTCATCCTCTCCATCGTGGG CACTGTGCTCTCGGACATCATCCAGAAGTACTTCTTCTCCCCGACGCTCTTCCGAGTCATCCGCCTGGCC CGAATAGGCCGCATCCTCAGACTGATCCGAGGGGCCAAGGGGATCCGCACGCTGCTCTTTGCCCTCATGA TGTCCCTGCCTGCCCTCTTCAACATCGGGCTGCTGCTCTTCCTCGTCATGTTCATCTACTCCATCTTTGG CATGGCCAACTTCGCTTATGTCAAGTGGGAGGCTGGCATCGACGACATGTTCAACTTCCAGACCTTCGCC AACAGCATGCTGTGCCTCTTCCAGATCACCACGTCGGCCGGCTGGGATGGCCTCCTCAGCCCCATCCTCA ACACTGGGCCGCCCTACTGCGACCCCACTCTGCCCAACAGCAATGGCTCTCGGGGGGACTGCGGGAGCCC AGCCGTGGGCATCCTCTTCTTCACCACCTACATCATCATCTCCTTCCTCATCGTGGTCAACATGTACATT GCCATCATCCTGGAGAACTTCAGCGTGGCCACGGAGGAGAGCACCGAGCCCCTGAGTGAGGACGACTTCG ATATGTTCTATGAGATCTGGGAGAAATTTGACCCAGAGGCCACTCAGTTTATTGAGTATTCGGTCCTGTC TGACTTTGCCGATGCCCTGTCTGAGCCACTCCGTATCGCCAAGCCCAACCAGATAAGCCTCATCAACATG GACCTGCCCATGGTGAGTGGGGACCGCATCCATTGCATGGACATTCTCTTTGCCTTCACCAAAAGGGTCC TGGGGGAGTCTGGGGAGATGGACGCCCTGAAGATCCAGATGGAGGAGAAGTTCATGGCAGCCAACCCATC CAAGATCTCCTACGAGCCCATCACCACCACACTCCGGCGCAAGCACGAAGAGGTGTCGGCCATGGTTATC CAGAGAGCCTTCCGCAGGCACCTGCTGCAACGCTCTTTGAAGCATGCCTCCTTCCTCTTCCGTCAGCAGG CGGGCAGCGGCCTCTCCGAAGAGGATGCCCCTGAGCGAGAGGGCCTCATCGCCTACGTGATGAGTGAGAA CTTCTCCCGACCCCTTGGCCCACCCTCCAGCTCCTCCATCTCCTCCACTTCCTTCCCACCCTCCTATGAC AGTGTCACTAGAGCCACCAGCGATAACCTCCAGGTGCGGGGGTCTGACTACAGCCACAGTGAAGATCTCG CCGACTTCCCCCCTTCTCCGGACAGGGACCGTGAGTCCATCGTG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG212010 representing NM_001099405
Red=Cloning site Green=Tags(s) MANFLLPRGTSSFRRFTRESLAAIEKRMAEKQARGSTTLQESREGLPEEEAPRPQLDLQASKKLPDLYGN PPQELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALYVLSPFHPIRRAAVKILVHSLFNMLIMCT ILTNCVFMAQHDPPPWTKYVEYTFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIIMAYVSENI KLGNLSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKC VRNFTALNGTNGSVEADGLVWESLDLYLSDPENYLLKNGTSDVLLCGNSSDAGTCPEGYRCLKAGENPDH GYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQN QATIAETEEKEKRFQEAMEMLKKEHEALTIRGVDTVSRSSLEMSPLAPVNSHERRSKRRKRMSSGTEECG EDRLPKSDSEDGPRAMNHLSLTRGLSRTSMKPRSSRGSIFTFRRRDLGSEADFADDENSTAGESESHHTS LLVPWPLRRTSAQGQPSPGTSAPGHALHGKKNSTVDCNGVVSLLGAGDPEATSPGSHLLRPVMLEHPPDT TTPSEEPGGPQMLTSQAPCVDGFEEPGARQRALSAVSVLTSALEELEESRHKCPPCWNRLAQRYLIWECC PLWMSIKQGVKLVVMDPFTDLTITMCIVLNTLFMALEHYNMTSEFEEMLQVGNLVFTGIFTAEMTFKIIA LDPYYYFQQGWNIFDSIIVILSLMELGLSRMSNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALG NLTLVLAIIVFIFAVVGMQLFGKNYSELRDSDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVS GQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTAPDEDREMNNLQLALARIQRGLRFVKRTTWDF CCGLLRQRPQKPAALAAQGQLPSCIATPYSPPPPETEKVPPTRKETRFEEGEQPGQGTPGDPEPVCVPIA VAESDTDDQEEDEENSLGTEEESSKQQESQPVSGGPEAPPDSRTWSQVSATASSEAEASASQADWRQQWK AEPQAPGCGETPEDSCSEGSTADMTNTAELLEQIPDLGQDVKDPEDCFTEGCVRRCPCCAVDTTQAPGKV WWRLRKTCYHIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVA YGFKKYFTNAWCWLDFLIVDVSLVSLVANTLGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGA IPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSQCESLNLTGELYWTKVKV NFDNVGAGYLALLQVYEEQPQWEYNLYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMT EEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLICLNMVTMMVETDDQSPEKIN ILAKINLLFVAIFTGECIVKLAALRHYYFTNSWNIFDFVVVILSIVGTVLSDIIQKYFFSPTLFRVIRLA RIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMANFAYVKWEAGIDDMFNFQTFA NSMLCLFQITTSAGWDGLLSPILNTGPPYCDPTLPNSNGSRGDCGSPAVGILFFTTYIIISFLIVVNMYI AIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPEATQFIEYSVLSDFADALSEPLRIAKPNQISLINM DLPMVSGDRIHCMDILFAFTKRVLGESGEMDALKIQMEEKFMAANPSKISYEPITTTLRRKHEEVSAMVI QRAFRRHLLQRSLKHASFLFRQQAGSGLSEEDAPEREGLIAYVMSENFSRPLGPPSSSSISSTSFPPSYD SVTRATSDNLQVRGSDYSHSEDLADFPPSPDRDRESIV SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001099405 |
| ORF Size | 5994 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001099405.2 |
| RefSeq Size | 8450 bp |
| RefSeq ORF | 5997 bp |
| Locus ID | 6331 |
| UniProt ID | Q14524 |
| Cytogenetics | 3p22.2 |
| Protein Families | Druggable Genome, Ion Channels: Sodium, Transmembrane |
| Gene Summary | The protein encoded by this gene is an integral membrane protein and tetrodotoxin-resistant voltage-gated sodium channel subunit. This protein is found primarily in cardiac muscle and is responsible for the initial upstroke of the action potential in an electrocardiogram. Defects in this gene are a cause of long QT syndrome type 3 (LQT3), an autosomal dominant cardiac disease. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
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