ANKS1B (NM_152788) Human Tagged ORF Clone

CAT#: RG211823

ANKS1B (tGFP-tagged) - Human ankyrin repeat and sterile alpha motif domain containing 1B (ANKS1B), transcript variant 1

Product Images

Circular map for RG211823

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ANKS1B
• Synonyms: AIDA; AIDA-1; ANKS2; cajalin-2; EB-1; EB1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211823 representing NM_152788
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAAGGACCAGGAGCTGCTGGAAGCTGCTCGCACTGGAAATGTGGCTCTGGTGGAGAAACTCCTGT
  CTGGCAGGAAAGGAGGGATCCTGGGCGGTGGATCCGGACCCCTGCCCCTGTCTAATCTGCTAAGCATCTG
  GCGAGGCCCCAATGTGAACTGCACAGACAGTTCGGGTTACACTGCTTTACACCACGCAGCCTTAAATGGA
  CATAAGGACATAGTTCTCAAACTACTTCAGTATGAGGCATCAACAAATGTAGCAGACAACAAAGGGTATT
  TTCCTATTCACCTGGCTGCCTGGAAAGGAGATGTGGAAATTGTGAAGATTCTTATTCATCATGGACCATC
  ACATTCCAGGGTCAATGAACAGAACAATGAAAATGAAACTGCCCTACACTGTGCAGCTCAATATGGACAC
  TCAGAAGTAGTTGCTGTTCTCCTAGAAGAGCTCACTGACCCGACAATTAGAAATAGCAAGCTGGAAACAC
  CTTTGGACTTGGCGGCACTCTACGGACGGCTTAGAGTGGTAAAAATGATCATCAGTGCACATCCTAACTT
  AATGAGCTGCAACACTCGCAAGCACACGCCACTTCACCTTGCTGCGCGCAATGGCCACAAAGCAGTCGTG
  CAGGTGCTGCTGGAGGCAGGAATGGATGTGAGCTGTCAAACAGAAAAGGGGAGTGCACTTCATGAAGCAG
  CTTTGTTTGGAAAGGTGGATGTTGTACGAGTTCTGTTAGAAACAGGAATTGATGCCAACATAAAGGATAG
  CTTAGGTAGAACTGTCTTGGACATTCTGAAAGAACATCCATCTCAGAAATCTCTCCAGATTGCAACACTC
  TTACAAGAGTATTTAGAAGGCGTGGGAAGATCTACAGTCCTCGAAGAGCCTGTACAGGAAGATGCAACAC
  AAGAAACACACATTTCATCTCCTGTTGAGTCTCCTTCCCAAAAGACCAAAAGTGAAACCGTCACTGGAGA
  ATTATCAAAACTCTTGGATGAAATAAAACTCTGTCAAGAAAAGGATTATTCGTTTGAAGACTTGTGCCAC
  ACAATATCAGACCACTACTTAGATAATTTGAGCAAGATTTCAGAGGAAGAACTTGGGAAAAATGGAAGCC
  AGAGTGTAAGAACCTCATCTACAATCAATTTGTCACCAGGAGAAGTGGAAGAAGAGGATGATGATGAAAA
  TACGTGTGGGCCATCAGGACTTTGGGAAGCATTAACTCCGTGTAATGGATGTAGGAACCTTGGCTTCCCC
  ATGCTTGCCCAGGAGTCCTATCCAAAGAAGAGAAATTACACTATGGAAATTGTACCATCTGCTTCTCTGG
  ATACATTTCCTTCAGAAAATGAGAACTTTCTGTGTGATCTCATGGACACAGCTGTTACAAAGAAACCTTG
  CTCCTTAGAAATTGCAAGGGCACCTTCCCCAAGAACTGATAATGCCTCTGAGGTAGCAGTTACTACTCCA
  GGAACTAGTAACCATAGAAACAGCTCAACAGGCCCAACACCTGATTGTTCACCTCCATCCCCTGATACTG
  CCCTCAAAAATATTGTAAAAGTCATTCGACCCCAGCCTAAACAGCGAACATCCATTGTGTCTTCTCTGGA
  TTTTCACCGAATGAATCACAACCAAGAATATTTTGAAATCAACACATCTACAGGGTGCACAAGCTTTACT
  GCCAGTCCTCCTGCTAGTCCACCCACCTCTTCTGTGGGAACCACAGAAGTCAAGAATGAGGGAACTAACC
  ATACAGATGACCTCTCCCGACAGGATGACAATGATCCCCCAAAAGAATATGATCCTGGGCAATTTGCAGG
  CCTGCTCCATGGATCCTCTCCAGCCTGTGAGTCCCCTGAAAATCCATTTCATCTCTATGGGAAAAGAGAA
  CAATGTGAAAAAGGACAAGATGAAGTCAGTTTGGCAAACAGTCCTTTGCCTTTCAAGCAGTCTCCAATAG
  AAAATAACTCAGAACCTTTGGTAAAGAAAATTAAACCCAAAGTGGTCAGTAGAACAATTTTTCACAAAAA
  AAGCAACCAACTCGAAAACCATACCATTGTTGGCACAAGATCAACCAGGAGTGGATCTCGGAATGGGGAC
  CAGTGGGTTATGAACGCAGGGGGATTTGTGGAGAGAGCCTGTACTCTGGGGAGAATAAGGTCATTGCCTA
  AAGCCTTGATCGACATGCATTTGTCAAAAAGTGTCTCTAAATCTGATTCTGATCTCATTGCCTATCCTTC
  CAATGAGAAAACATCAAGAGTTAACTGGAGTGAATCTTCCACTGCTGAACACAGTTCTAAAGGGAATTCT
  GAAAGAACACCATCCTTCACATCGGAATGGGAAGAAATTGACAAAATAATGAGTTCCATAGATGTTGGAA
  TCAACAACGAACTTAAAGAGATGAATGGTGAGACCACAAGACCCAGATGCCCTGTCCAAACAGTGGGACA
  ATGGTTGGAAAGCATTGGGCTACCTCAGTACGAGAACCACCTGATGGCTAATGGATTTGACAATGTGCAG
  TTTATGGGAAGCAATGTTATGGAAGATCAGGATTTGTTGGAAATTGGAATCCTTAATTCTGGGCACAGAC
  AAAGAATTCTACAGGCAATCCAGCTCCTTCCAAAGATGAGACCCATTGGGCATGATGGCTACCATCCCAC
  CTCTGTAGCTGAGTGGCTGGATTCCATTGAACTGGGCGACTACACCAAAGCCTTTCTAATTAATGGCTAC
  ACTTCGATGGACCTGTTGAAAAAAATCTGGGAGGTTGAACTTATTAATGTTTTAAAAATCAATTTGATTG
  GCCACAGGAAACGTATTTTGGCATCTCTGGGAGACAGGCTGCACGACGATCCCCCACAGAAGCCCCCTCG
  GTCCATCACCCTCAGGGAACCCAGTGGTAATCACACTCCTCCTCAGTTGTCTCCATCACTTAGCCAAAGC
  ACTTACACCACTGGTGGCTCCCTAGACGTTCCTCACATTATCATGCAGGGCGATGCAAGGAGGAGAAGAA
  ATGAAAACTACTTTGATGATATTCCCCGATCAAAACTGGAGAGGCAGATGGCTCAGACAGGAGACTGGGG
  AGAACCTTCCATTACCTTGCGACCTCCGAATGAAGCCACAGCCTCTACCCCGGTACAGTACTGGCAGCAT
  CACCCAGAAAAGCTTATCTTCCAGTCGTGTGATTACAAAGCTTTTTATTTAGGTTCTATGCTGATAAAAG
  AGCTTAGGGGGACAGAATCAACCCAAGATGCTTGTGCAAAAATGCGGGCTAACTGTCAGAAGTCTACAGA
  GCAAATGAAGAAGGTCCCTACTATTATTCTTTCTGTCTCATATAAAGGAGTCAAATTTATTGATGCAACA
  AATAAGAACATAATTGCTGAGCATGAAATTCGTAATATCTCCTGTGCTGCCCAGGACCCAGAAGACCTCT
  CAACATTTGCCTATATCACAAAAGATTTGAAGTCTAATCACCACTACTGTCATGTGTTTACTGCCTTTGA
  TGTGAATTTAGCCTATGAAATCATCCTAACCCTGGGACAGGCATTCGAAGTCGCTTACCAGCTAGCACTA
  CAAGCAAGAAAAGGGGGACACTCCTCCACACTTCCAGAAAGCTTTGAAAACAAACCCTCCAAACCCATCC
  CCAAGCCCCGCGTTAGCATTCGCAAGTCCGTGGATCTTCTTCATGCCAGCCATACCGGGCAGGAACCATC
  AGAGAGACACACTGAGGAGGCACTCAGAAAATTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211823 representing NM_152788
  Red=Cloning site Green=Tags(s)
  
  MGKDQELLEAARTGNVALVEKLLSGRKGGILGGGSGPLPLSNLLSIWRGPNVNCTDSSGYTALHHAALNG
  HKDIVLKLLQYEASTNVADNKGYFPIHLAAWKGDVEIVKILIHHGPSHSRVNEQNNENETALHCAAQYGH
  SEVVAVLLEELTDPTIRNSKLETPLDLAALYGRLRVVKMIISAHPNLMSCNTRKHTPLHLAARNGHKAVV
  QVLLEAGMDVSCQTEKGSALHEAALFGKVDVVRVLLETGIDANIKDSLGRTVLDILKEHPSQKSLQIATL
  LQEYLEGVGRSTVLEEPVQEDATQETHISSPVESPSQKTKSETVTGELSKLLDEIKLCQEKDYSFEDLCH
  TISDHYLDNLSKISEEELGKNGSQSVRTSSTINLSPGEVEEEDDDENTCGPSGLWEALTPCNGCRNLGFP
  MLAQESYPKKRNYTMEIVPSASLDTFPSENENFLCDLMDTAVTKKPCSLEIARAPSPRTDNASEVAVTTP
  GTSNHRNSSTGPTPDCSPPSPDTALKNIVKVIRPQPKQRTSIVSSLDFHRMNHNQEYFEINTSTGCTSFT
  ASPPASPPTSSVGTTEVKNEGTNHTDDLSRQDDNDPPKEYDPGQFAGLLHGSSPACESPENPFHLYGKRE
  QCEKGQDEVSLANSPLPFKQSPIENNSEPLVKKIKPKVVSRTIFHKKSNQLENHTIVGTRSTRSGSRNGD
  QWVMNAGGFVERACTLGRIRSLPKALIDMHLSKSVSKSDSDLIAYPSNEKTSRVNWSESSTAEHSSKGNS
  ERTPSFTSEWEEIDKIMSSIDVGINNELKEMNGETTRPRCPVQTVGQWLESIGLPQYENHLMANGFDNVQ
  FMGSNVMEDQDLLEIGILNSGHRQRILQAIQLLPKMRPIGHDGYHPTSVAEWLDSIELGDYTKAFLINGY
  TSMDLLKKIWEVELINVLKINLIGHRKRILASLGDRLHDDPPQKPPRSITLREPSGNHTPPQLSPSLSQS
  TYTTGGSLDVPHIIMQGDARRRRNENYFDDIPRSKLERQMAQTGDWGEPSITLRPPNEATASTPVQYWQH
  HPEKLIFQSCDYKAFYLGSMLIKELRGTESTQDACAKMRANCQKSTEQMKKVPTIILSVSYKGVKFIDAT
  NKNIIAEHEIRNISCAAQDPEDLSTFAYITKDLKSNHHYCHVFTAFDVNLAYEIILTLGQAFEVAYQLAL
  QARKGGHSSTLPESFENKPSKPIPKPRVSIRKSVDLLHASHTGQEPSERHTEEALRKF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152788
• ORF Size: 3744 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152788.4, NP_690001.3
• RefSeq Size: 4431 bp
• RefSeq ORF: 3747 bp
• Locus ID: 56899
• UniProt ID: Q7Z6G8
• Cytogenetics: 12q23.1
• Domains: SAM, PID
• Gene Summary: This gene encodes a multi-domain protein that is predominantly expressed in brain and testis. This protein interacts with amyloid beta protein precursor (AbetaPP) and may have a role in normal brain development, and in the pathogenesis of Alzheimer's disease. Expression of this gene has been shown to be elevated in patients with pre-B cell acute lymphocytic leukemia associated with t(1;19) translocation. Alternatively spliced transcript variants encoding different isoforms (some with different subcellular localization, PMID:15004329) have been described for this gene. [provided by RefSeq, Aug 2011]