NDST4 (NM_022569) Human Tagged ORF Clone

CAT#: RG211521

NDST4 (tGFP-tagged) - Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 4 (NDST4)

Product Images

Circular map for RG211521

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NDST4
• Synonyms: N-HSST; N-HSST 4; NDST-4; NHSST4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211521 representing NM_022569
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCTTATTGTGAAACTTCGGAGAAGTTTTCGAACATTGATTGTTCTCTTAGCTACCTTTTGCTTGG
  TGAGCATTGTCATTTCTGCCTATTTTCTCTACTCTGGCTACAAACAGGAAATGACACTTATTGAAACCAC
  TGCAGAAGCAGAATGCACTGACATCAAAATTCTACCATATAGGTCAATGGAGCTGAAAACAGTTAAACCT
  ATTGACACATCCAAAACGGACCCTACTGTCCTTCTCTTCGTGGAGAGCCAATACTCTCAACTCGGTCAAG
  ATATCATAGCTATTTTGGAGTCCAGCCGATTTCAGTACCACATGGTTATTGCCCCTGGAAAGGGAGATAT
  ACCTCCTCTTACAGATAATGGCAAAGGGAAATATACTTTAGTTATTTATGAAAATATTCTGAAGTATGTC
  AGCATGGACTCATGGAATCGAGAGCTTTTAGAAAAATACTGTGTGGAATACAGTGTTAGTATAATCGGTT
  TTCATAAAGCCAATGAGAACAGCTTACCAAGTACACAATTAAAAGGCTTTCCGTTAAACCTTTTCAATAA
  TCTAGCTCTAAAGGACTGTTTTGTTAACCCTCAATCTCCTTTGCTGCATATTACCAAAGCCCCCAAGGTT
  GAGAAAGGCCCTCTTCCTGGGGAAGACTGGACTATTTTCCAATATAATCATTCAACCTACCAGCCTGTAC
  TTTTAACTGAGTTACAGACAGAAAAATCCCTGTCATCCTTGTCTAGCAAAACACTCTTTGCAACGGTGAT
  TCAGGATCTGGGGCTTCATGATGGAATTCAGAGAGTACTTTTTGGCAACAACTTGAACTTTTGGCTGCAC
  AAGCTCATCTTCATAGATGCCATCTCCTTCTTGTCAGGGAAGAGGCTGACATTGTCCTTGGACAGGTACA
  TCCTTGTGGACATTGATGATATATTTGTTGGGAAAGAGGGAACAAGGATGAATGTCAAAGATGTGAAGGC
  ATTACTAGAGACTCAAAATTTACTGCGCACTCAGGTTGCAAATTTTACCTTCAACCTTGGATTTTCAGGG
  AAGTTTTACCACACAGGGACTGAAGAGGAAGATGAAGGAGATGACCTTTTACTTCGGTCTGTGGATGAGT
  TCTGGTGGTTTCCTCACATGTGGAGCCACATGCAGCCCCACCTCTTCCACAACGAGTCATCTTTAGTAGA
  GCAGATGATTCTCAACAAGGAATTTGCACTGGAACATGGAATACCAATCAACATGGGCTATGCTGTGGCC
  CCACATCACTCAGGGGTCTACCCGGTTCACATTCAGCTGTATGCAGCTTGGAAGAAGGTCTGGGGTATTC
  AAGTCACCAGCACTGAAGAATATCCACATCTGAAACCTGCCCGGTACAGAAAGGGCTTCATTCACAATAG
  CATCATGGTCCTCCCTCGACAGACTTGTGGGTTGTTCACTCACACTATTTTCTACAAAGAATATCCAGGA
  GGACCCCAAGAACTGGATAAAAGTATCAGAGGAGGTGAACTTTTTCTCACAATCCTTCTAAACCCAATCA
  GCATTTTCATGACCCATTTATCAAACTATGGAAATGACCGCCTAGGGTTATATACCTTTGTGAACTTGGT
  CAACTTTGTGCAGAGCTGGACCAACCTGAAATTGCAAACTCTGCCTCCAGTGCAACTGGCCCACCAGTAT
  TTTGAGCTCTTCCCTGAGCAGAAAGACCCTCTATGGCAGAATCCATGTGATGATAAACGACACAAAGACA
  TCTGGTCCAGAGAGAAAACTTGTGACCACTTACCAAAATTTCTAGTAATTGGTCCACAAAAAACAGGAAC
  AACTGCACTTTATTTATTTCTTCTTATGCATCCTTCAATCATCAGCAATCTCCCTAGTCCAAAGACATTT
  GAGGAAGTTCAGTTCTTTAATGGCAACAACTATCACAAAGGAATAGACTGGTATATGGACTTTTTCCCTA
  CACCATCCAATACTACAAGTGACTTTCTGTTTGAAAAGAGTGCTAATTACTTCCATTCGGAAGAAGCTCC
  AAGACGAGCCGCATCTCTTGTCCCCAAAGCCAAGATCATCACCATCCTCATTGACCCCTCAGACAGGGCA
  TACTCTTGGTACCAGCACCAACGATCACATGAAGATCCAGCTGCTCTGAGGTTCAATTTCTATGAAGTTA
  TTTCAACAGGACATTGGGCTCCATCTGACTTAAAAACTTTGCAGAGAAGATGCCTAGTACCTGGATGGTA
  TGCAGTCCACATAGAAAGATGGCTAACTTACTTTGCTACTTCTCAGTTGCTAATTATTGATGGACAGCAG
  CTGAGATCTGACCCAGCTACTGTGATGGATGAAGTCCAGAAGTTTCTGGGAGTTACACCTCGTTATAATT
  ACTCTGAAGCACTAACGTTTGATCCCCAAAAGGGTTTTTGGTGTCAATTACTGGAAGGAGGAAAGACAAA
  ATGCCTTGGAAAAAGCAAAGGCCGAAAATATCCACCTATGGATCCAGAGTCTAGAACGTTCCTCTCTAAT
  TACTACCGAGATCATAATGTGGAACTATCCAAACTGCTACACAGACTGGGACAGCCTCTGCCATCGTGGC
  TGAGACAGGAACTGCAGAAAGTGAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211521 representing NM_022569
  Red=Cloning site Green=Tags(s)
  
  MNLIVKLRRSFRTLIVLLATFCLVSIVISAYFLYSGYKQEMTLIETTAEAECTDIKILPYRSMELKTVKP
  IDTSKTDPTVLLFVESQYSQLGQDIIAILESSRFQYHMVIAPGKGDIPPLTDNGKGKYTLVIYENILKYV
  SMDSWNRELLEKYCVEYSVSIIGFHKANENSLPSTQLKGFPLNLFNNLALKDCFVNPQSPLLHITKAPKV
  EKGPLPGEDWTIFQYNHSTYQPVLLTELQTEKSLSSLSSKTLFATVIQDLGLHDGIQRVLFGNNLNFWLH
  KLIFIDAISFLSGKRLTLSLDRYILVDIDDIFVGKEGTRMNVKDVKALLETQNLLRTQVANFTFNLGFSG
  KFYHTGTEEEDEGDDLLLRSVDEFWWFPHMWSHMQPHLFHNESSLVEQMILNKEFALEHGIPINMGYAVA
  PHHSGVYPVHIQLYAAWKKVWGIQVTSTEEYPHLKPARYRKGFIHNSIMVLPRQTCGLFTHTIFYKEYPG
  GPQELDKSIRGGELFLTILLNPISIFMTHLSNYGNDRLGLYTFVNLVNFVQSWTNLKLQTLPPVQLAHQY
  FELFPEQKDPLWQNPCDDKRHKDIWSREKTCDHLPKFLVIGPQKTGTTALYLFLLMHPSIISNLPSPKTF
  EEVQFFNGNNYHKGIDWYMDFFPTPSNTTSDFLFEKSANYFHSEEAPRRAASLVPKAKIITILIDPSDRA
  YSWYQHQRSHEDPAALRFNFYEVISTGHWAPSDLKTLQRRCLVPGWYAVHIERWLTYFATSQLLIIDGQQ
  LRSDPATVMDEVQKFLGVTPRYNYSEALTFDPQKGFWCQLLEGGKTKCLGKSKGRKYPPMDPESRTFLSN
  YYRDHNVELSKLLHRLGQPLPSWLRQELQKVR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022569
• ORF Size: 2616 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022569.3
• RefSeq Size: 3359 bp
• RefSeq ORF: 2619 bp
• Locus ID: 64579
• UniProt ID: Q9H3R1
• Cytogenetics: 4q26
• Protein Families: Transmembrane
• Protein Pathways: Heparan sulfate biosynthesis, Metabolic pathways
• Gene Summary: Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Has low deacetylase activity but high sulfotransferase activity (By similarity).[UniProtKB/Swiss-Prot Function]