Cyclin B2 (CCNB2) (NM_004701) Human Tagged ORF Clone

CAT#: RG211260

CCNB2 (tGFP-tagged) - Human cyclin B2 (CCNB2)

Product Images

Circular map for RG211260

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cyclin B2
• Synonyms: HsT17299
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211260 representing NM_004701
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTGCTCCGACGCCCGACGGTGTCCAGTGATTTGGAGAATATTGACACAGGAGTTAATTCTAAAG
  TTAAGAGTCATGTGACTATTAGGCGAACTGTTTTAGAAGAAATTGGAAATAGAGTTACAACCAGAGCAGC
  ACAAGTAGCTAAGAAAGCTCAGAACACCAAAGTTCCAGTTCAACCCACCAAAACAACAAATGTCAACAAA
  CAACTGAAACCTACTGCTTCTGTCAAACCAGTACAGATGGAAAAGTTGGCTCCAAAGGGTCCTTCTCCCA
  CACCTGAGGATGTCTCCATGAAGGAAGAGAATCTCTGCCAAGCTTTTTCTGATGCCTTGCTCTGCAAAAT
  CGAGGACATTGATAACGAAGATTGGGAGAACCCTCAGCTCTGCAGTGACTACGTTAAGGATATCTATCAG
  TATCTCAGGCAGCTGGAGGTTTTGCAGTCCATAAACCCACATTTCTTAGATGGAAGAGATATAAATGGAC
  GCATGCGTGCCATCCTAGTGGATTGGCTGGTACAAGTCCACTCCAAGTTTAGGCTTCTGCAGGAGACTCT
  GTACATGTGCGTTGGCATTATGGATCGATTTTTACAGGTTCAGCCAGTTTCCCGGAAGAAGCTTCAATTA
  GTTGGGATTACTGCTCTGCTCTTGGCTTCCAAGTATGAGGAGATGTTTTCTCCAAATATTGAAGACTTTG
  TTTACATCACAGACAATGCTTATACCAGTTCCCAAATCCGAGAAATGGAAACTCTAATTTTGAAAGAATT
  GAAATTTGAGTTGGGTCGACCCTTGCCACTACACTTCTTAAGGCGAGCATCAAAAGCCGGGGAGGTTGAT
  GTTGAACAGCACACTTTAGCCAAGTATTTGATGGAGCTGACTCTCATCGACTATGATATGGTGCATTATC
  ATCCTTCTAAGGTAGCAGCAGCTGCTTCCTGCTTGTCTCAGAAGGTTCTAGGACAAGGAAAATGGAACTT
  AAAGCAGCAGTATTACACAGGATACACAGAGAATGAAGTATTGGAAGTCATGCAGCACATGGCCAAGAAT
  GTGGTGAAAGTAAATGAAAACTTAACTAAATTCATCGCCATCAAGAATAAGTATGCAAGCAGCAAACTCC
  TGAAGATCAGCATGATCCCTCAGCTGAACTCAAAAGCCGTCAAAGACCTTGCCTCCCCACTGATAGGAAG
  GTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211260 representing NM_004701
  Red=Cloning site Green=Tags(s)
  
  MALLRRPTVSSDLENIDTGVNSKVKSHVTIRRTVLEEIGNRVTTRAAQVAKKAQNTKVPVQPTKTTNVNK
  QLKPTASVKPVQMEKLAPKGPSPTPEDVSMKEENLCQAFSDALLCKIEDIDNEDWENPQLCSDYVKDIYQ
  YLRQLEVLQSINPHFLDGRDINGRMRAILVDWLVQVHSKFRLLQETLYMCVGIMDRFLQVQPVSRKKLQL
  VGITALLLASKYEEMFSPNIEDFVYITDNAYTSSQIREMETLILKELKFELGRPLPLHFLRRASKAGEVD
  VEQHTLAKYLMELTLIDYDMVHYHPSKVAAAASCLSQKVLGQGKWNLKQQYYTGYTENEVLEVMQHMAKN
  VVKVNENLTKFIAIKNKYASSKLLKISMIPQLNSKAVKDLASPLIGRS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004701
• ORF Size: 1194 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004701.4
• RefSeq Size: 1530 bp
• RefSeq ORF: 1197 bp
• Locus ID: 9133
• UniProt ID: O95067
• Cytogenetics: 15q22.2
• Domains: cyclin_C, CYCLIN, cyclin
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Cell cycle, Oocyte meiosis, p53 signaling pathway, Progesterone-mediated oocyte maturation
• Gene Summary: Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control. [provided by RefSeq, Jul 2008]