Epsin 2 (EPN2) (NM_148921) Human Tagged ORF Clone

CAT#: RG210899

EPN2 (tGFP-tagged) - Human epsin 2 (EPN2), transcript variant 1

Product Images

Circular map for RG210899

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Epsin 2
• Synonyms: EHB21
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210899 representing NM_148921
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGACTTCGTCTATCAGACGGCAGATGAAAAACATCGTGAACAATTACTCAGAGGCAGAAATCAAAG
  TCCGGGAAGCCACCTCCAATGACCCGTGGGGCCCGTCCAGTTCTCTGATGACCGAGATTGCCGACCTGAC
  CTACAACGTGGTGGCCTTCTCGGAGATCATGAGCATGGTGTGGAAGCGGCTGAATGACCATGGCAAGAAC
  TGGCGGCATGTGTACAAGGCGCTGACCCTGCTGGACTACCTCATCAAGACAGGCTCCGAACGTGTGGCCC
  AGCAGTGCCGGGAGAACATCTTCGCCATCCAGACCCTGAAGGACTTCCAGTACATTGACCGAGATGGCAA
  GGACCAGGGCATCAATGTGCGTGAGAAGTCAAAGCAACTGGTGGCTCTCCTCAAGGACGAGGAACGGTTG
  AAGGCTGAGAGGGCCCAGGCTCTCAAAACCAAAGAGCGCATGGCCCAGGTTGCCACTGGCATGGGCAGCA
  ACCAGATCACCTTTGGGCGAGGCTCCAGCCAGCCCAACCTCTCCACCAGCCACTCGGAGCAGGAGTATGG
  CAAGGCCGGGGGCTCCCCGGCCTCCTACCATGGCTCCACCTCCCCGCGAGTGTCCTCCGAGCTGGAGCAA
  GCCCGGCCCCAGACTAGTGGAGAAGAGGAGCTTCAGCTGCAGCTGGCACTTGCCATGAGCAGAGAAGTGG
  CTGAGCAGGAAGAACGCCTCAGGCGGGGTGATGACCTCAGATTACAGATGGCCCTGGAAGAAAGCCGAAG
  GGACACAGTTAAAATTCCAAAAAAGAAAGAGCATGGCTCTCTCCCACAGCAGACTACGCTGTTGGATTTA
  ATGGATGCTCTCCCCAGCTCGGGCCCCGCGGCCCAGAAAGCAGAGCCCTGGGGCCCGTCAGCCTCCACTA
  ACCAGACCAACCCCTGGGGCGGGCCAGCGGCTCCTGCGAGTACTTCAGACCCCTGGCCATCGTTTGGTAC
  CAAGCCAGCTGCCTCCATTGACCCATGGGGGGTGCCCACTGGAGCCACCGCACAATCTGTCCCCAAGAAC
  TCGGACCCCTGGGCAGCTTCACAGCAGCCTGCCTCCAGTGCTGGGAAAAGAGCTTCTGACGCGTGGGGCG
  CAGTCTCCACCACCAAGCCCGTGTCTGTCTCTGGGTCCTTTGAGCTCTTCAGTAATCTGAATGGTACAAT
  TAAAGATGACTTTTCTGAATTTGACAACCTTCGGACTTCAAAAAAAACAGCCGAATCTGTGACCTCTCTG
  CCATCCCAAAACAATGGAACTACCAGCCCTGACCCCTTTGAGTCTCAACCCCTGACTGTCGCCTCAAGCA
  AGCCCAGCAGTGCCCGGAAAACACCTGAGTCCTTCCTGGGCCCCAACGCGGCCCTGGTGAACCTGGACTC
  ACTGGTGACCAGGCCTGCCCCACCAGCCCAGTCCCTCAACCCTTTCCTGGCACCAGGTGCTCCCGCCACC
  TCGGCCCCTGTTAACCCTTTCCAGGTGAACCAGCCCCAGCCGCTGACACTGAACCAGCTTCGGGGGAGCC
  CAGTCCTGGGGACCAGCACATCCTTTGGGCCTGGCCCAGGAGTGGAGTCCATGGCTGTGGCCTCGATGAC
  CTCCGCGGCCCCACAGCCAGCTCTGGGGGCCACTGGTTCCTCTCTGACACCACTGGGCCCTGCAATGATG
  AACATGGTGGGCAGTGTGGGTATACCCCCATCAGCAGCCCAGGCCACTGGCACAACCAACCCTTTCCTTC
  TC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210899 representing NM_148921
  Red=Cloning site Green=Tags(s)
  
  MTTSSIRRQMKNIVNNYSEAEIKVREATSNDPWGPSSSLMTEIADLTYNVVAFSEIMSMVWKRLNDHGKN
  WRHVYKALTLLDYLIKTGSERVAQQCRENIFAIQTLKDFQYIDRDGKDQGINVREKSKQLVALLKDEERL
  KAERAQALKTKERMAQVATGMGSNQITFGRGSSQPNLSTSHSEQEYGKAGGSPASYHGSTSPRVSSELEQ
  ARPQTSGEEELQLQLALAMSREVAEQEERLRRGDDLRLQMALEESRRDTVKIPKKKEHGSLPQQTTLLDL
  MDALPSSGPAAQKAEPWGPSASTNQTNPWGGPAAPASTSDPWPSFGTKPAASIDPWGVPTGATAQSVPKN
  SDPWAASQQPASSAGKRASDAWGAVSTTKPVSVSGSFELFSNLNGTIKDDFSEFDNLRTSKKTAESVTSL
  PSQNNGTTSPDPFESQPLTVASSKPSSARKTPESFLGPNAALVNLDSLVTRPAPPAQSLNPFLAPGAPAT
  SAPVNPFQVNQPQPLTLNQLRGSPVLGTSTSFGPGPGVESMAVASMTSAAPQPALGATGSSLTPLGPAMM
  NMVGSVGIPPSAAQATGTTNPFLL
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_148921
• ORF Size: 1752 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_148921.2, NP_683723.1
• RefSeq Size: 4643 bp
• RefSeq ORF: 1755 bp
• Locus ID: 22905
• UniProt ID: O95208
• Cytogenetics: 17p11.2
• Domains: ENTH, UIM
• Protein Pathways: Endocytosis
• Gene Summary: This gene encodes a protein which interacts with clathrin and adaptor-related protein complex 2, alpha 1 subunit. The protein is found in a brain-derived clathrin-coated vesicle fraction and localizes to the peri-Golgi region and the cell periphery. The protein is thought to be involved in clathrin-mediated endocytosis. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]