Epsin 2 (EPN2) (NM_148921) Human Untagged Clone

CAT#: SC310244

EPN2 (untagged)-Human epsin 2 (EPN2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Epsin 2
• Synonyms: EHB21
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC310244 representing NM_148921.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGACGACTTCGTCTATCAGACGGCAGATGAAAAACATCGTGAACAATTACTCAGAGGCAGAAATCAAA
  GTCCGGGAAGCCACCTCCAATGACCCGTGGGGCCCGTCCAGTTCTCTGATGACCGAGATTGCCGACCTG
  ACCTACAACGTGGTGGCCTTCTCGGAGATCATGAGCATGGTGTGGAAGCGGCTGAATGACCATGGCAAG
  AACTGGCGGCATGTGTACAAGGCGCTGACCCTGCTGGACTACCTCATCAAGACAGGCTCCGAACGTGTG
  GCCCAGCAGTGCCGGGAGAACATCTTCGCCATCCAGACCCTGAAGGACTTCCAGTACATTGACCGAGAT
  GGCAAGGACCAGGGCATCAATGTGCGTGAGAAGTCAAAGCAACTGGTGGCTCTCCTCAAGGACGAGGAA
  CGGTTGAAGGCTGAGAGGGCCCAGGCTCTCAAAACCAAAGAGCGCATGGCCCAGGTTGCCACTGGCATG
  GGCAGCAACCAGATCACCTTTGGGCGAGGCTCCAGCCAGCCCAACCTCTCCACCAGCCACTCGGAGCAG
  GAGTATGGCAAGGCCGGGGGCTCCCCGGCCTCCTACCATGGCTCCACCTCCCCGCGAGTGTCCTCCGAG
  CTGGAGCAAGCCCGGCCCCAGACTAGTGGAGAAGAGGAGCTTCAGCTGCAGCTGGCACTTGCCATGAGC
  AGAGAAGTGGCTGAGCAGGAAGAACGCCTCAGGCGGGGTGATGACCTCAGATTACAGATGGCCCTGGAA
  GAAAGCCGAAGGGACACAGTTAAAATTCCAAAAAAGAAAGAGCATGGCTCTCTCCCACAGCAGACTACG
  CTGTTGGATTTAATGGATGCTCTCCCCAGCTCGGGCCCCGCGGCCCAGAAAGCAGAGCCCTGGGGCCCG
  TCAGCCTCCACTAACCAGACCAACCCCTGGGGCGGGCCAGCGGCTCCTGCGAGTACTTCAGACCCCTGG
  CCATCGTTTGGTACCAAGCCAGCTGCCTCCATTGACCCATGGGGGGTGCCCACTGGAGCCACCGTACAA
  TCTGTCCCCAAGAACTCGGACCCCTGGGCAGCTTCACAGCAGCCTGCCTCCAGTGCTGGGAAAAGAGCT
  TCTGACGCGTGGGGCGCAGTCTCCACCACCAAGCCCGTGTCTGTCTCTGGGTCCTTTGAGCTCTTCAGT
  AATCTGAATGGTACAATTAAAGATGACTTTTCTGAATTTGACAACCTTCGGACTTCAAAAAAAACAGCC
  GAATCTGTGACCTCTCTGCCATCCCAAAACAATGGAACTACCAGCCCTGACCCCTTTGAGTCTCAACCC
  CTGACTGTCGCCTCAAGCAAGCCCAGCAGTGCCCGGAAAACACCTGAGTCCTTCCTGGGCCCCAACGCG
  GCCCTGGTGAACCTGGACTCACTGGTGACCAGGCCTGCCCCACCAGCCCAGTCCCTCAACCCTTTCCTG
  GCACCAGGTGCTCCCGCCACCTCGGCCCCTGTTAACCCTTTCCAGGTGAACCAGCCCCAGCCGCTGACA
  CTGAACCAGCTTCGGGGGAGCCCAGTCCTGGGGACCAGCACATCCTTTGGGCCTGGCCCAGGAGTGGAG
  TCCATGGCTGTGGCCTCGATGACCTCCGCGGCCCCACAGCCAGCTCTGGGGGCCACTGGTTCCTCTCTG
  ACACCACTGGGCCCTGCAATGATGAACATGGTGGGCAGTGTGGGTATACCCCCATCAGCAGCCCAGGCC
  ACTGGCACAACCAACCCTTTCCTTCTCTAG
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_148921
• Insert Size: 1755 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_148921.3
• RefSeq Size: 4664 bp
• RefSeq ORF: 1755 bp
• Locus ID: 22905
• UniProt ID: O95208
• Cytogenetics: 17p11.2
• Domains: ENTH, UIM
• Protein Pathways: Endocytosis
• MW: 62.3 kDa
• Gene Summary: This gene encodes a protein which interacts with clathrin and adaptor-related protein complex 2, alpha 1 subunit. The protein is found in a brain-derived clathrin-coated vesicle fraction and localizes to the peri-Golgi region and the cell periphery. The protein is thought to be involved in clathrin-mediated endocytosis. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) lacks an alternate in-frame exon compared to variant 2. The resulting isoform (a) has the same N- and C-termini but is shorter compared to isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.