Kv1.6 (KCNA6) (NM_002235) Human Tagged ORF Clone

CAT#: RG210186

KCNA6 (tGFP-tagged) - Human potassium voltage-gated channel, shaker-related subfamily, member 6 (KCNA6)

Product Images

Circular map for RG210186

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Kv1.6
• Synonyms: HBK2; KV1.6; PPP1R96
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210186 representing NM_002235
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGATCGGAGAAATCCCTTACGCTGGCGGCGCCGGGGGAGGTCCGTGGGCCGGAGGGAGAGCAACAGG
  ATGCGGGAGACTTCCCGGAGGCCGGCGGGGGCGGGGGCTGCTGTAGTAGCGAGCGGCTGGTGATCAATAT
  CTCCGGGCTGCGCTTTGAGACACAATTGCGCACCCTGTCGCTGTTTCCGGACACGCTGCTCGGAGACCCT
  GGCCGGCGAGTCCGCTTCTTCGACCCCCTGAGGAACGAGTACTTCTTCGACCGCAACCGGCCCAGCTTCG
  ACGCCATCCTCTACTACTACCAGTCTGGGGGCCGCCTGCGGAGGCCGGTCAACGTGCCCCTGGACATTTT
  CCTGGAGGAGATCCGCTTCTACCAGCTGGGGGACGAGGCCCTGGCGGCCTTCCGGGAGGACGAGGGCTGC
  CTGCCCGAAGGTGGCGAGGACGAGAAGCCGCTGCCCTCCCAGCCCTTCCAGCGCCAGGTGTGGCTGCTCT
  TTGAGTACCCAGAGAGCTCTGGGCCGGCCAGGGGCATCGCCATCGTCTCCGTGTTGGTCATTCTCATCTC
  CATAGTCATCTTTTGCCTGGAGACCTTACCCCAGTTCCGTGTAGATGGTCGAGGTGGAAACAATGGTGGT
  GTGAGTCGAGTCTCCCCAGTTTCCAGGGGGAGTCAGGAGGAAGAGGAGGATGAAGACGATTCCTACACAT
  TTCATCATGGCATCACCCCTGGGGAAATGGGGACCGGGGGCTCCTCCTCACTCAGTACTCTTGGGGGCTC
  CTTCTTTACAGACCCCTTCTTTCTGGTGGAGACGCTGTGCATTGTCTGGTTCACTTTTGAGCTCCTGGTG
  CGCTTCTCCGCCTGCCCTAGCAAGCCGGCCTTCTTCCGGAACATCATGAACATCATTGACTTGGTGGCTA
  TCTTCCCCTACTTCATCACCCTGGGCACTGAGCTGGTGCAGCAGCAGGAGCAGCAACCAGCCAGTGGAGG
  AGGCGGCCAGAATGGGCAGCAGGCCATGTCCCTGGCCATCCTCCGAGTCATCCGCCTGGTCCGGGTGTTC
  CGCATCTTCAAGCTCTCCCGCCACTCCAAGGGGCTGCAGATCCTGGGCAAGACCTTGCAGGCCTCCATGA
  GGGAGCTGGGGCTGCTCATCTTCTTCCTCTTCATCGGGGTCATCCTCTTCTCCAGTGCCGTCTACTTCGC
  AGAGGCTGACGATGACGATTCGCTTTTTCCCAGCATCCCGGATGCCTTCTGGTGGGCAGTGGTTACAATG
  ACCACGGTAGGTTACGGGGACATGTACCCCATGACTGTGGGGGGAAAGATCGTGGGCTCGCTGTGTGCCA
  TCGCTGGGGTCCTCACCATTGCCCTGCCTGTGCCCGTCATCGTCTCCAACTTCAACTACTTCTACCACCG
  GGAGACGGAGCAGGAGGAGCAAGGCCAGTATACCCACGTCACTTGTGGGCAGCCTGCGCCGGACCTGAGG
  GCAACTGACAACGGACTTGGCAAGCCTGACTTCCCCGAGGCTAACCGGGAACGGAGACCCAGCTACCTTC
  CTACACCACATCGGGCCTATGCAGAGAAAAGAATGCTCACGGAGGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210186 representing NM_002235
  Red=Cloning site Green=Tags(s)
  
  MRSEKSLTLAAPGEVRGPEGEQQDAGDFPEAGGGGGCCSSERLVINISGLRFETQLRTLSLFPDTLLGDP
  GRRVRFFDPLRNEYFFDRNRPSFDAILYYYQSGGRLRRPVNVPLDIFLEEIRFYQLGDEALAAFREDEGC
  LPEGGEDEKPLPSQPFQRQVWLLFEYPESSGPARGIAIVSVLVILISIVIFCLETLPQFRVDGRGGNNGG
  VSRVSPVSRGSQEEEEDEDDSYTFHHGITPGEMGTGGSSSLSTLGGSFFTDPFFLVETLCIVWFTFELLV
  RFSACPSKPAFFRNIMNIIDLVAIFPYFITLGTELVQQQEQQPASGGGGQNGQQAMSLAILRVIRLVRVF
  RIFKLSRHSKGLQILGKTLQASMRELGLLIFFLFIGVILFSSAVYFAEADDDDSLFPSIPDAFWWAVVTM
  TTVGYGDMYPMTVGGKIVGSLCAIAGVLTIALPVPVIVSNFNYFYHRETEQEEQGQYTHVTCGQPAPDLR
  ATDNGLGKPDFPEANRERRPSYLPTPHRAYAEKRMLTEV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002235
• ORF Size: 1587 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002235.3, NP_002226.1
• RefSeq Size: 4237 bp
• RefSeq ORF: 1590 bp
• Locus ID: 3742
• UniProt ID: P17658
• Cytogenetics: 12p13.32
• Domains: BTB, K_tetra, ion_trans
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the delayed rectifier class. The coding region of this gene is intronless, and the gene is clustered with genes KCNA1 and KCNA5 on chromosome 12. [provided by RefSeq, Jul 2008]