PI 3 Kinase p85 beta (PIK3R2) (NM_005027) Human Tagged ORF Clone

CAT#: RG209834

PIK3R2 (tGFP-tagged) - Human phosphoinositide-3-kinase, regulatory subunit 2 (beta) (PIK3R2)

Product Images

Circular map for RG209834

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PI 3 Kinase p85 beta
• Synonyms: MPPH; MPPH1; p85; p85-BETA; P85B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209834 representing NM_005027
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGCCCTGAGGGCTTCCAGTACCGCGCTCTGTACCCGTTCCGCCGGGAGCGGCCGGAGGACCTGG
  AGCTGCTGCCCGGCGACGTGCTGGTAGTGAGCCGGGCGGCCTTGCAGGCGCTGGGCGTGGCCGAGGGTGG
  CGAGCGCTGCCCACAGAGCGTGGGCTGGATGCCCGGCCTCAACGAGCGCACACGGCAGCGAGGTGACTTC
  CCTGGCACCTATGTGGAGTTCCTGGGGCCCGTGGCCCTGGCCCGGCCCGGCCCTCGCCCACGGGGCCCCC
  GCCCACTGCCCGCCAGGCCCCGTGATGGGGCCCCTGAGCCAGGCCTCACACTCCCCGACTTGCCCGAGCA
  GTTCTCCCCACCTGATGTGGCTCCCCCTCTTCTGGTGAAGCTTGTGGAGGCCATTGAAAGGACAGGGCTG
  GACAGCGAATCTCACTACCGCCCGGAGCTGCCCGCACCGCGTACAGACTGGTCCCTGAGCGACGTGGATC
  AGTGGGACACGGCAGCCCTGGCTGACGGCATTAAGAGCTTCCTGCTGGCACTGCCCGCGCCGCTCGTGAC
  CCCCGAGGCCTCGGCCGAGGCGCGCCGGGCCCTGCGGGAGGCCGCGGGGCCCGTGGGGCCGGCGCTGGAG
  CCACCGACGCTGCCGCTGCACCGCGCGCTCACGCTGCGCTTCCTGCTCCAGCACCTGGGCCGCGTGGCCC
  GCCGCGCCCCGGCCCTGGGTCCCGCGGTCCGGGCCCTGGGCGCCACCTTTGGGCCGCTGCTGCTGCGCGC
  GCCGCCGCCGCCGTCCTCGCCGCCGCCAGGGGGCGCTCCCGACGGGAGTGAGCCCAGCCCTGACTTCCCG
  GCGCTGCTGGTGGAGAAGCTGCTTCAGGAACACTTGGAAGAGCAGGAGGTTGCGCCCCCAGCGCTGCCGC
  CTAAACCCCCCAAGGCAAAGCCGGCCCCCACAGTCCTGGCCAATGGAGGGAGCCCACCCTCCCTGCAGGA
  TGCTGAGTGGTACTGGGGGGACATTTCAAGGGAGGAGGTGAACGAGAAACTCCGGGACACTCCCGATGGC
  ACCTTCCTAGTCCGAGATGCTTCTAGCAAGATCCAGGGCGAGTACACGCTGACCCTCAGGAAAGGCGGGA
  ACAATAAGCTGATCAAGGTCTTCCACCGAGATGGGCACTATGGCTTCTCAGAGCCACTCACCTTCTGCTC
  CGTTGTGGACCTCATCAATCACTACCGCCACGAGTCTCTGGCCCAGTACAATGCCAAGCTGGACACACGG
  CTCCTCTACCCTGTGTCCAAATACCAGCAGGACCAGATTGTCAAGGAGGACAGCGTGGAGGCAGTGGGCG
  CCCAGCTTAAGGTCTATCACCAGCAGTACCAGGACAAGAGCCGCGAGTATGACCAGCTTTATGAAGAGTA
  CACACGGACCTCCCAGGAGCTGCAGATGAAGCGTACTGCAATTGAGGCCTTCAATGAGACTATCAAGATC
  TTTGAAGAGCAGGGCCAGACTCAAGAGAAATGCAGCAAGGAATACCTGGAGCGCTTCCGGCGTGAGGGCA
  ACGAGAAAGAGATGCAAAGGATCCTGCTGAACTCCGAGCGGCTCAAGTCCCGCATTGCCGAGATCCATGA
  GAGCCGCACGAAGCTGGAGCAGCAGCTGCGGGCCCAGGCCTCGGACAACAGAGAGATCGACAAGCGCATG
  AACAGCCTCAAGCCGGACCTCATGCAGCTGCGCAAGATCCGAGACCAGTACCTCGTGTGGCTCACCCAGA
  AAGGCGCCCGGCAGAAGAAAATCAACGAGTGGCTGGGGATTAAAAATGAGACTGAGGACCAGTACGCACT
  CATGGAGGACGAGGACGATCTCCCGCACCACGAGGAACGCACTTGGTACGTGGGCAAGATCAACCGCACG
  CAGGCAGAGGAGATGCTGAGTGGCAAGCGGGATGGCACCTTCCTCATCCGCGAGAGCAGCCAGCGGGGCT
  GCTACGCCTGCTCCGTGGTAGTGGACGGCGACACCAAGCACTGCGTCATCTACCGCACGGCCACCGGCTT
  CGGCTTCGCGGAGCCCTACAACCTGTACGGGTCGCTGAAGGAGCTGGTGCTGCACTACCAGCACGCCTCG
  CTGGTGCAGCACAACGACGCGCTCACCGTCACCCTGGCGCACCCAGTGCGCGCCCCGGGCCCCGGCCCGC
  CGCCTGCCGCCCGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209834 representing NM_005027
  Red=Cloning site Green=Tags(s)
  
  MAGPEGFQYRALYPFRRERPEDLELLPGDVLVVSRAALQALGVAEGGERCPQSVGWMPGLNERTRQRGDF
  PGTYVEFLGPVALARPGPRPRGPRPLPARPRDGAPEPGLTLPDLPEQFSPPDVAPPLLVKLVEAIERTGL
  DSESHYRPELPAPRTDWSLSDVDQWDTAALADGIKSFLLALPAPLVTPEASAEARRALREAAGPVGPALE
  PPTLPLHRALTLRFLLQHLGRVARRAPALGPAVRALGATFGPLLLRAPPPPSSPPPGGAPDGSEPSPDFP
  ALLVEKLLQEHLEEQEVAPPALPPKPPKAKPAPTVLANGGSPPSLQDAEWYWGDISREEVNEKLRDTPDG
  TFLVRDASSKIQGEYTLTLRKGGNNKLIKVFHRDGHYGFSEPLTFCSVVDLINHYRHESLAQYNAKLDTR
  LLYPVSKYQQDQIVKEDSVEAVGAQLKVYHQQYQDKSREYDQLYEEYTRTSQELQMKRTAIEAFNETIKI
  FEEQGQTQEKCSKEYLERFRREGNEKEMQRILLNSERLKSRIAEIHESRTKLEQQLRAQASDNREIDKRM
  NSLKPDLMQLRKIRDQYLVWLTQKGARQKKINEWLGIKNETEDQYALMEDEDDLPHHEERTWYVGKINRT
  QAEEMLSGKRDGTFLIRESSQRGCYACSVVVDGDTKHCVIYRTATGFGFAEPYNLYGSLKELVLHYQHAS
  LVQHNDALTVTLAHPVRAPGPGPPPAAR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005027
• ORF Size: 2184 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005027.3
• RefSeq Size: 3953 bp
• RefSeq ORF: 2187 bp
• Locus ID: 5296
• UniProt ID: O00459
• Cytogenetics: 19p13.11
• Domains: RhoGAP, SH2, SH3
• Protein Families: Druggable Genome
• Protein Pathways: Acute myeloid leukemia, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Insulin signaling pathway, Jak-STAT signaling pathway, Leukocyte transendothelial migration, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Phosphatidylinositol signaling system, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus, VEGF signaling pathway
• Gene Summary: Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates phosphatidylinositol and similar compounds, creating second messengers important in growth signaling pathways. PI3K functions as a heterodimer of a regulatory and a catalytic subunit. The protein encoded by this gene is a regulatory component of PI3K. Three transcript variants, one protein coding and the other two non-protein coding, have been found for this gene. [provided by RefSeq, Apr 2019]