CNKSR3 (NM_173515) Human Tagged ORF Clone

CAT#: RG209167

CNKSR3 (tGFP-tagged) - Human CNKSR family member 3 (CNKSR3)

Product Images

Circular map for RG209167

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CNKSR3
• Synonyms: CNK3; CNK3/IPCEF1; MAGI1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209167 representing NM_173515
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCCGTGACCAAGTGGAGCCCCAAACAAGTGGTGGACTGGACTAGAGGGTTGGATGACTGCCTGC
  AACAATATGTCCACAAGTTTGAACGAGAGAAGATCAACGGCGAGCAGCTGCTGCAGATTTCCCATCAGGA
  CCTGGAGGAGCTGGGGGTCACACGGATCGGACACCAGGAGCTTGTGTTGGAGGCTGTGGACCTTCTCTGT
  GCACTGAATTATGGCCTCGAAACTGATAACATGAAGAACTTGGTTCTGAAACTGAGAGCATCTTCCCACA
  ATTTACAGAATTACATAAGTAGCCGACGGAAAAGTCCCGCTTACGATGGCAACACCTCCCGCAAGGCCCC
  CAATGAGTTCCTGACCTCGGTGGTGGAGCTCATCGGCGCCGCCAAGGCCCTGCTGGCGTGGCTGGACCGG
  GCTCCGTTTACAGGGATCACTGATTTCTCAGTCACGAAGAACAAAATTATCCAGCTTTGCTTGGACCTGA
  CCACTACAGTCCAGAAGGATTGCTTTGTAGCGGAAATGGAGGATAAAGTTTTAACTGTGGTCAAGGTTTT
  AAATGGCATCTGTGACAAAACAATCCGATCTACCACAGATCCTGTGATGAGCCAGTGTGCATGTCTGGAG
  GAAGTTCACTTACCAAACATTAAACCTGGGGAAGGCCTGGGCATGTACATCAAATCAACCTATGATGGGT
  TACACGTGATTACTGGAACCACAGAAAATTCTCCTGCAGACAGATCTCAGAAGATTCATGCTGGTGACGA
  AGTCATTCAAGTTAATCAGCAAACTGTGGTGGGATGGCAGCTGAAAAATCTGGTGAAGAAATTGAGAGAG
  AATCCCACCGGAGTTGTGTTACTGCTTAAGAAGCGCCCCACCGGGTCTTTCAACTTTACTCCTGCTCCCC
  TGAAAAACCTACGGTGGAAGCCACCTCTTGTACAGACCTCACCTCCACCCGCGACAACCCAGTCCCCTGA
  AAGCACTATGGATACCTCACTGAAGAAGGAGAAGTCAGCCATCCTGGATCTTTATATTCCTCCTCCGCCT
  GCTGTTCCCTACTCTCCCCGGGATGAGAATGGCAGTTTTGTTTATGGAGGGTCCAGTAAGTGCAAACAAC
  CATTGCCTGGTCCTAAGGGTTCAGAGTCCCCGAATTCCTTCTTGGACCAGGAAAGCCGGAGACGAAGATT
  CACCATTGCAGACTCGGATCAGTTGCCTGGGTACTCGGTGGAAACCAACATTCTGCCCACAAAAATGAGA
  GAGAAAACACCATCTTATGGCAAGCCACGGCCTTTGTCCATGCCTGCTGATGGGAACTGGATGGGGATTG
  TGGACCCTTTTGCCAGACCTCGAGGTCATGGCAGGAAAGGGGAGGATGCCCTTTGCCGGTATTTCAGTAA
  CGAGCGGATTCCTCCGATCATTGAAGAGAGCTCCTCTCCCCCATACCGGTTCTCCAGACCCACGACCGAG
  CGGCATCTGGTCCGGGGTGCGGACTACATCCGAGGAAGCAGGTGCTACATCAACTCAGATCTCCACAGCA
  GCGCCACGATTCCATTCCAGGAGGAAGGGACCAAAAAGAAATCTGGCTCCTCAGCTACGAAGTCCTCGTC
  CACAGAACCGTCCCTCCTGGTCAGCTGGTTTACGCGCCTCAAACTGTTGACTCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209167 representing NM_173515
  Red=Cloning site Green=Tags(s)
  
  MEPVTKWSPKQVVDWTRGLDDCLQQYVHKFEREKINGEQLLQISHQDLEELGVTRIGHQELVLEAVDLLC
  ALNYGLETDNMKNLVLKLRASSHNLQNYISSRRKSPAYDGNTSRKAPNEFLTSVVELIGAAKALLAWLDR
  APFTGITDFSVTKNKIIQLCLDLTTTVQKDCFVAEMEDKVLTVVKVLNGICDKTIRSTTDPVMSQCACLE
  EVHLPNIKPGEGLGMYIKSTYDGLHVITGTTENSPADRSQKIHAGDEVIQVNQQTVVGWQLKNLVKKLRE
  NPTGVVLLLKKRPTGSFNFTPAPLKNLRWKPPLVQTSPPPATTQSPESTMDTSLKKEKSAILDLYIPPPP
  AVPYSPRDENGSFVYGGSSKCKQPLPGPKGSESPNSFLDQESRRRRFTIADSDQLPGYSVETNILPTKMR
  EKTPSYGKPRPLSMPADGNWMGIVDPFARPRGHGRKGEDALCRYFSNERIPPIIEESSSPPYRFSRPTTE
  RHLVRGADYIRGSRCYINSDLHSSATIPFQEEGTKKKSGSSATKSSSTEPSLLVSWFTRLKLLTH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173515
• ORF Size: 1665 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173515.2, NP_775786.2
• RefSeq Size: 3277 bp
• RefSeq ORF: 1668 bp
• Locus ID: 154043
• UniProt ID: Q6P9H4
• Cytogenetics: 6q25.2
• Protein Families: Druggable Genome
• Gene Summary: Involved in transepithelial sodium transport. Regulates aldosterone-induced and epithelial sodium channel (ENaC)-mediated sodium transport through regulation of ENaC cell surface expression. Acts as a scaffold protein coordinating the assembly of an ENaC-regulatory complex (ERC).[UniProtKB/Swiss-Prot Function]