Phospholipase D1 (PLD1) (NM_002662) Human Tagged ORF Clone

CAT#: RG209070

PLD1 (tGFP-tagged) - Human phospholipase D1, phosphatidylcholine-specific (PLD1), transcript variant 1

Product Images

Circular map for RG209070

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Phospholipase D1
• Synonyms: CVDD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209070 representing NM_002662
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACTGAAAAACGAGCCACGGGTAAATACCTCTGCACTGCAGAAAATTGCTGCTGACATGAGTAATA
  TCATAGAAAATCTGGACACGCGGGAACTCCACTTTGAGGGAGAGGAGGTAGACTACGACGTGTCTCCCAG
  CGATCCCAAGATACAAGAAGTGTATATCCCTTTCTCTGCTATTTATAACACTCAAGGATTTAAGGAGCCT
  AATATACAGACGTATCTCTCCGGCTGTCCAATAAAAGCACAAGTTCTGGAAGTGGAACGCTTCACATCTA
  CAACAAGGGTACCAAGTATTAATCTTTACACTATTGAATTAACACATGGGGAATTTAAATGGCAAGTTAA
  GAGGAAATTCAAGCATTTTCAAGAATTTCACAGAGAGCTGCTCAAGTACAAAGCCTTTATCCGCATCCCC
  ATTCCCACTAGAAGACACACGTTTAGGAGGCAAAACGTCAGAGAGGAGCCTCGAGAGATGCCCAGTTTGC
  CCCGTTCATCTGAAAACATGATAAGAGAAGAACAATTCCTTGGTAGAAGAAAACAACTGGAAGATTACTT
  GACAAAGATACTAAAAATGCCCATGTATAGAAACTATCATGCCACAACAGAGTTTCTTGATATAAGCCAG
  CTGTCTTTCATCCATGATTTGGGACCAAAGGGCATAGAAGGTATGATAATGAAAAGATCTGGAGGACACA
  GAATACCAGGCTTGAATTGCTGTGGTCAGGGAAGAGCCTGCTACAGATGGTCAAAAAGATGGTTAATAGT
  GAAAGATTCCTTTTTATTGTATATGAAACCAGACAGCGGTGCCATTGCCTTCGTCCTGCTGGTAGACAAA
  GAATTCAAAATTAAGGTGGGGAAGAAGGAGACAGAAACGAAATATGGAATCCGAATTGATAATCTTTCAA
  GGACACTTATTTTAAAATGCAACAGCTATAGACATGCTCGGTGGTGGGGAGGGGCTATAGAAGAATTCAT
  CCAGAAACATGGCACCAACTTTCTCAAAGATCATCGATTTGGGTCATATGCTGCTATCCAAGAGAATGCT
  TTAGCTAAATGGTATGTTAATGCCAAAGGATATTTTGAAGATGTGGCAAATGCAATGGAAGAGGCAAATG
  AAGAGATTTTTATCACAGACTGGTGGCTGAGTCCAGAAATCTTCCTGAAACGCCCAGTGGTTGAGGGAAA
  TCGTTGGAGGTTGGACTGCATTCTTAAACGAAAAGCACAACAAGGAGTGAGGATCTTCATAATGCTCTAC
  AAAGAGGTGGAACTCGCTCTTGGCATCAATAGTGAATACACCAAGAGGACTTTGATGCGTCTACATCCCA
  ACATAAAGGTGATGAGACACCCGGATCATGTGTCATCCACCGTCTATTTGTGGGCTCACCATGAGAAGCT
  TGTCATCATTGACCAATCGGTGGCCTTTGTGGGAGGGATTGACCTGGCCTATGGAAGGTGGGACGACAAT
  GAGCACAGACTCACAGACGTGGGCAGTGTGAAGCGGGTCACTTCAGGACCGTCTCTGGGTTCCCTCCCAC
  CTGCCGCAATGGAGTCTATGGAATCCTTAAGACTCAAAGATAAAAATGAGCCTGTTCAAAACCTACCCAT
  CCAGAAGAGTATTGATGATGTGGATTCAAAACTGAAAGGAATAGGAAAGCCAAGAAAGTTCTCCAAATTT
  AGTCTCTACAAGCAGCTCCACAGGCACCACCTGCACGACGCAGATAGCATCAGCAGCATTGACAGCACCT
  CCAGTTATTTTAATCACTATAGAAGTCATCACAATTTAATCCATGGTTTAAAACCCCACTTCAAACTCTT
  TCACCCGTCCAGTGAGTCTGAGCAAGGACTCACTAGACCTCATGCTGATACCGGGTCCATCCGTAGTTTA
  CAGACAGGTGTGGGAGAGCTGCATGGGGAAACCAGATTCTGGCATGGAAAGGACTACTGCAATTTCGTCT
  TCAAAGACTGGGTTCAACTTGATAAACCTTTTGCTGATTTCATTGACAGGTACTCCACGCCCCGGATGCC
  CTGGCATGACATTGCCTCTGCAGTCCACGGGAAGGCGGCTCGTGATGTGGCACGTCACTTCATCCAGCGC
  TGGAACTTCACAAAAATTATGAAATCAAAATATCGGTCCCTTTCTTATCCTTTTCTGCTTCCAAAGTCTC
  AAACAACAGCCCATGAGTTGAGATATCAAGTGCCTGGGTCTGTCCATGCTAACGTACAGTTGCTCCGCTC
  TGCTGCTGATTGGTCTGCTGGTATAAAGTACCATGAAGAGTCCATCCACGCCGCTTACGTCCATGTGATA
  GAGAACAGCAGGCACTATATCTATATCGAAAACCAGTTTTTCATAAGCTGTGCTGATGACAAAGTTGTGT
  TCAACAAGATAGGCGATGCCATTGCCCAGAGGATCCTGAAAGCTCACAGGGAAAACCAGAAATACCGGGT
  ATATGTCGTGATACCACTTCTGCCAGGGTTCGAAGGAGACATTTCAACCGGCGGAGGAAATGCTCTACAG
  GCAATCATGCACTTCAACTACAGAACCATGTGCAGAGGAGAAAATTCCATCCTTGGACAGTTAAAAGCAG
  AGCTTGGTAATCAGTGGATAAATTACATATCATTCTGTGGTCTTAGAACACATGCAGAGCTCGAAGGAAA
  CCTAGTAACTGAGCTTATCTATGTCCACAGCAAGTTGTTAATTGCTGATGATAACACTGTTATTATTGGC
  TCTGCCAACATAAATGACCGCAGCATGCTGGGAAAGCGTGACAGTGAAATGGCTGTCATTGTGCAAGATA
  CAGAGACTGTTCCTTCAGTAATGGATGGAAAAGAGTACCAAGCTGGCCGGTTTGCCCGAGGACTTCGGCT
  ACAGTGCTTTAGGGTTGTCCTTGGCTATCTTGATGACCCAAGTGAGGACATTCAGGATCCAGTGAGTGAC
  AAATTCTTCAAGGAGGTGTGGGTTTCAACAGCAGCTCGAAATGCTACAATTTATGACAAGGTTTTCCGGT
  GCCTTCCCAATGATGAAGTACACAATTTAATTCAGCTGAGAGACTTTATAAACAAGCCCGTATTAGCTAA
  GGAAGATCCCATTCGAGCTGAGGAGGAACTGAAGAAGATCCGTGGATTTTTGGTGCAATTCCCCTTTTAT
  TTCTTGTCTGAAGAAAGCCTACTGCCTTCTGTTGGGACCAAAGAGGCCATAGTGCCCATGGAGGTTTGGA
  CT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209070 representing NM_002662
  Red=Cloning site Green=Tags(s)
  
  MSLKNEPRVNTSALQKIAADMSNIIENLDTRELHFEGEEVDYDVSPSDPKIQEVYIPFSAIYNTQGFKEP
  NIQTYLSGCPIKAQVLEVERFTSTTRVPSINLYTIELTHGEFKWQVKRKFKHFQEFHRELLKYKAFIRIP
  IPTRRHTFRRQNVREEPREMPSLPRSSENMIREEQFLGRRKQLEDYLTKILKMPMYRNYHATTEFLDISQ
  LSFIHDLGPKGIEGMIMKRSGGHRIPGLNCCGQGRACYRWSKRWLIVKDSFLLYMKPDSGAIAFVLLVDK
  EFKIKVGKKETETKYGIRIDNLSRTLILKCNSYRHARWWGGAIEEFIQKHGTNFLKDHRFGSYAAIQENA
  LAKWYVNAKGYFEDVANAMEEANEEIFITDWWLSPEIFLKRPVVEGNRWRLDCILKRKAQQGVRIFIMLY
  KEVELALGINSEYTKRTLMRLHPNIKVMRHPDHVSSTVYLWAHHEKLVIIDQSVAFVGGIDLAYGRWDDN
  EHRLTDVGSVKRVTSGPSLGSLPPAAMESMESLRLKDKNEPVQNLPIQKSIDDVDSKLKGIGKPRKFSKF
  SLYKQLHRHHLHDADSISSIDSTSSYFNHYRSHHNLIHGLKPHFKLFHPSSESEQGLTRPHADTGSIRSL
  QTGVGELHGETRFWHGKDYCNFVFKDWVQLDKPFADFIDRYSTPRMPWHDIASAVHGKAARDVARHFIQR
  WNFTKIMKSKYRSLSYPFLLPKSQTTAHELRYQVPGSVHANVQLLRSAADWSAGIKYHEESIHAAYVHVI
  ENSRHYIYIENQFFISCADDKVVFNKIGDAIAQRILKAHRENQKYRVYVVIPLLPGFEGDISTGGGNALQ
  AIMHFNYRTMCRGENSILGQLKAELGNQWINYISFCGLRTHAELEGNLVTELIYVHSKLLIADDNTVIIG
  SANINDRSMLGKRDSEMAVIVQDTETVPSVMDGKEYQAGRFARGLRLQCFRVVLGYLDDPSEDIQDPVSD
  KFFKEVWVSTAARNATIYDKVFRCLPNDEVHNLIQLRDFINKPVLAKEDPIRAEEELKKIRGFLVQFPFY
  FLSEESLLPSVGTKEAIVPMEVWT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002662
• ORF Size: 3222 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002662.5
• RefSeq Size: 5494 bp
• RefSeq ORF: 3225 bp
• Locus ID: 5337
• UniProt ID: Q13393
• Cytogenetics: 3q26.31
• Protein Pathways: Endocytosis, Ether lipid metabolism, Fc gamma R-mediated phagocytosis, Glycerophospholipid metabolism, GnRH signaling pathway, Metabolic pathways, Pancreatic cancer, Pathways in cancer
• Gene Summary: This gene encodes a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. The enzyme may play a role in signal transduction and subcellular trafficking. Alternative splicing results in multiple transcript variants with both catalytic and regulatory properties. [provided by RefSeq, Sep 2011]