PNPT1 (NM_033109) Human Tagged ORF Clone

CAT#: RG208800

PNPT1 (tGFP-tagged) - Human polyribonucleotide nucleotidyltransferase 1 (PNPT1)

Product Images

Circular map for RG208800

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PNPT1
• Synonyms: COXPD13; DFNB70; old-35; OLD35; PNPASE
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208800 representing NM_033109
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCTGCAGGTACTGCTGCTCGTGCCTCCGGCTCCGGCCCCTGAGCGATGGTCCTTTCCTTCTGC
  CACGGCGGGATCGGGCACTCACCCAGTTGCAAGTGCGAGCACTATGGAGTAGCGCAGGGTCTCGAGCTGT
  GGCCGTGGACTTAGGCAACAGGAAATTAGAAATATCTTCTGGAAAGCTGGCCAGATTTGCAGATGGCTCT
  GCTGTAGTACAGTCAGGTGACACTGCAGTAATGGTCACAGCGGTCAGTAAAACAAAACCTTCCCCTTCCC
  AGTTTATGCCTTTGGTGGTTGACTACAGACAAAAAGCTGCTGCAGCAGGTAGAATTCCCACAAACTATCT
  GAGAAGAGAGATTGGTACTTCTGATAAAGAAATTCTAACAAGTCGAATAATAGATCGTTCAATTAGACCG
  CTCTTTCCAGCTGGCTACTTCTATGATACACAGGTTCTGTGTAATCTGTTAGCAGTAGATGGTGTAAATG
  AGCCTGATGTCCTAGCAATTAATGGCGCTTCCGTAGCCCTCTCATTATCAGATATTCCTTGGAATGGACC
  TGTTGGGGCAGTACGAATAGGAATAATTGATGGAGAATATGTTGTTAACCCAACAAGAAAAGAAATGTCT
  TCTAGTACTTTAAATTTAGTGGTTGCTGGAGCACCTAAAAGTCAGATTGTCATGTTGGAAGCCTCTGCAG
  AGAACATTTTACAGCAGGACTTTTGCCATGCTATCAAAGTGGGAGTGAAATATACCCAACAAATAATTCA
  GGGCATTCAGCAGTTGGTAAAAGAAACTGGTGTTACCAAGAGGACACCTCAGAAGTTATTTACCCCTTCG
  CCAGAGATTGTGAAATATACTCATAAACTTGCTATGGAGAGACTCTATGCAGTTTTTACAGATTACGAGC
  ATGACAAAGTTTCCAGAGATGAAGCTGTTAACAAAATAAGATTAGATACGGAGGAACAACTAAAAGAAAA
  ATTTCCAGAAGCCGATCCATATGAAATAATAGAATCCTTCAATGTTGTTGCAAAGGAAGTTTTTAGAAGT
  ATTGTTTTGAATGAATACAAAAGGTGCGATGGTCGGGATTTGACTTCACTTAGGAATGTAAGTTGTGAGG
  TAGATATGTTTAAAACCCTTCATGGATCAGCATTATTTCAAAGAGGACAAACACAGGTGCTTTGTACCGT
  TACATTTGATTCATTAGAATCTGGTATTAAGTCAGATCAAGTTATAACAGCTATAAATGGGATAAAAGAT
  AAAAATTTCATGCTGCACTACGAGTTTCCTCCTTATGCAACTAATGAAATTGGCAAAGTCACTGGTTTAA
  ATAGAAGAGAACTTGGGCATGGTGCTCTTGCTGAGAAAGCTTTGTATCCTGTTATTCCCCGAGATTTTCC
  TTTCACCATAAGAGTTACATCTGAAGTCCTAGAGTCAAATGGGTCATCTTCTATGGCATCTGCATGTGGC
  GGAAGTTTAGCATTAATGGATTCAGGGGTTCCAATTTCATCTGCTGTTGCAGGCGTAGCAATAGGATTGG
  TCACCAAAACCGATCCTGAGAAGGGTGAAATAGAAGATTATCGTTTGCTGACAGATATTTTGGGAATTGA
  AGATTACAATGGTGACATGGACTTCAAAATAGCTGGCACTAATAAAGGAATAACTGCATTACAGGCTGAT
  ATTAAATTACCTGGAATACCAATAAAAATTGTGATGGAGGCTATTCAACAAGCTTCAGTGGCAAAAAAGG
  AGATATTACAGATCATGAACAAAACTATTTCAAAACCTCGAGCATCTAGAAAAGAAAATGGACCTGTTGT
  AGAAACTGTTCAGGTTCCATTATCAAAACGAGCAAAATTTGTTGGACCTGGTGGCTATAACTTAAAAAAA
  CTTCAGGCTGAAACAGGTGTAACTATTAGTCAGGTGGATGAAGAAACGTTTTCTGTATTTGCACCAACAC
  CCAGTGCTATGCATGAGGCAAGAGACTTCATTACTGAAATCTGCAAGGATGATCAGGAGCAGCAATTAGA
  ATTTGGAGCAGTATATACCGCCACAATAACTGAAATCAGAGATACTGGTGTAATGGTAAAATTATATCCA
  AATATGACTGCGGTACTGCTTCATAACACACAACTTGATCAACGAAAGATTAAACATCCTACTGCCCTAG
  GATTAGAAGTTGGCCAAGAAATTCAGGTGAAATACTTTGGACGTGACCCAGCCGATGGAAGAATGAGGCT
  TTCTCGAAAAGTGCTTCAGTCGCCAGCTACAACCGTGGTCAGAACTTTGAATGACAGAAGTAGTATTGTA
  ATGGGAGAACCTATTTCACAGTCATCATCTAATTCTCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208800 representing NM_033109
  Red=Cloning site Green=Tags(s)
  
  MAACRYCCSCLRLRPLSDGPFLLPRRDRALTQLQVRALWSSAGSRAVAVDLGNRKLEISSGKLARFADGS
  AVVQSGDTAVMVTAVSKTKPSPSQFMPLVVDYRQKAAAAGRIPTNYLRREIGTSDKEILTSRIIDRSIRP
  LFPAGYFYDTQVLCNLLAVDGVNEPDVLAINGASVALSLSDIPWNGPVGAVRIGIIDGEYVVNPTRKEMS
  SSTLNLVVAGAPKSQIVMLEASAENILQQDFCHAIKVGVKYTQQIIQGIQQLVKETGVTKRTPQKLFTPS
  PEIVKYTHKLAMERLYAVFTDYEHDKVSRDEAVNKIRLDTEEQLKEKFPEADPYEIIESFNVVAKEVFRS
  IVLNEYKRCDGRDLTSLRNVSCEVDMFKTLHGSALFQRGQTQVLCTVTFDSLESGIKSDQVITAINGIKD
  KNFMLHYEFPPYATNEIGKVTGLNRRELGHGALAEKALYPVIPRDFPFTIRVTSEVLESNGSSSMASACG
  GSLALMDSGVPISSAVAGVAIGLVTKTDPEKGEIEDYRLLTDILGIEDYNGDMDFKIAGTNKGITALQAD
  IKLPGIPIKIVMEAIQQASVAKKEILQIMNKTISKPRASRKENGPVVETVQVPLSKRAKFVGPGGYNLKK
  LQAETGVTISQVDEETFSVFAPTPSAMHEARDFITEICKDDQEQQLEFGAVYTATITEIRDTGVMVKLYP
  NMTAVLLHNTQLDQRKIKHPTALGLEVGQEIQVKYFGRDPADGRMRLSRKVLQSPATTVVRTLNDRSSIV
  MGEPISQSSSNSQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033109
• ORF Size: 2349 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033109.5
• RefSeq Size: 4579 bp
• RefSeq ORF: 2352 bp
• Locus ID: 87178
• UniProt ID: Q8TCS8
• Cytogenetics: 2p16.1
• Domains: RNase_PH_C
• Protein Pathways: Purine metabolism, Pyrimidine metabolism, RNA degradation
• Gene Summary: The protein encoded by this gene belongs to the evolutionary conserved polynucleotide phosphorylase family comprised of phosphate dependent 3'-to-5' exoribonucleases implicated in RNA processing and degradation. This enzyme is predominantly localized in the mitochondrial intermembrane space and is involved in import of RNA to mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency-13 and autosomal recessive nonsyndromic deafness-70. Related pseudogenes are found on chromosomes 3 and 7. [provided by RefSeq, Dec 2012]