GSK3 alpha (GSK3A) (NM_019884) Human Tagged ORF Clone

CAT#: RG208698

GSK3A (tGFP-tagged) - Human glycogen synthase kinase 3 alpha (GSK3A)

Product Images

Circular map for RG208698

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GSK3 alpha
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208698 representing NM_019884
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGGCGGCGGGCCTTCGGGAGGCGGCCCTGGGGGCTCGGGCAGGGCGCGGACTAGCTCGTTCGCGG
  AGCCCGGCGGCGGAGGCGGAGGAGGCGGCGGCGGCCCCGGAGGCTCGGCCTCCGGCCCAGGCGGCACCGG
  CGGCGGAAAGGCATCTGTCGGGGCCATGGGTGGGGGCGTCGGGGCCTCGAGCTCCGGGGGTGGACCCGGC
  GGCAGCGGCGGAGGAGGCAGCGGAGGCCCCGGCGCAGGCACTAGCTTCCCGCCGCCCGGGGTGAAGCTGG
  GCCGTGACAGCGGGAAGGTGACCACAGTCGTAGCCACTCTAGGCCAAGGCCCAGAGCGCTCCCAAGAAGT
  GGCTTACACGGACATCAAAGTGATTGGCAATGGCTCATTTGGGGTCGTGTACCAGGCACGGCTGGCAGAG
  ACCAGGGAACTAGTCGCCATCAAGAAGGTTCTCCAGGACAAGAGGTTCAAGAACCGAGAGCTGCAGATCA
  TGCGTAAGCTGGACCACTGCAATATTGTGAGGCTGAGATACTTTTTCTACTCCAGTGGCGAGAAGAAAGA
  CGAGCTTTACCTAAATCTGGTGCTGGAATATGTGCCCGAGACAGTGTACCGGGTGGCCCGCCACTTCACC
  AAGGCCAAGTTGACCATCCCTATCCTCTATGTCAAGGTGTACATGTACCAGCTCTTCCGCAGCTTGGCCT
  ACATCCACTCCCAGGGCGTGTGTCACCGCGACATCAAGCCCCAGAACCTGCTGGTGGACCCTGACACTGC
  TGTCCTCAAGCTCTGCGATTTTGGCAGTGCAAAGCAGTTGGTCCGAGGGGAGCCCAATGTCTCCTACATC
  TGTTCTCGCTACTACCGGGCCCCAGAGCTCATCTTTGGAGCCACTGATTACACCTCATCCATCGATGTTT
  GGTCAGCTGGCTGTGTACTGGCAGAGCTCCTCTTGGGCCAGCCCATCTTCCCTGGGGACAGTGGGGTGGA
  CCAGCTGGTGGAGATCATCAAGGTGCTGGGAACACCAACCCGGGAACAAATCCGAGAGATGAACCCCAAC
  TACACGGAGTTCAAGTTCCCTCAGATTAAAGCTCACCCCTGGACAAAGGTGTTCAAATCTCGAACGCCGC
  CAGAGGCCATCGCGCTCTGCTCTAGCCTGCTGGAGTACACCCCATCCTCAAGGCTCTCCCCACTAGAGGC
  CTGTGCGCACAGCTTCTTTGATGAACTGCGATGTCTGGGAACCCAGCTGCCTAACAACCGCCCACTTCCC
  CCTCTCTTCAACTTCAGTGCTGGTGAACTCTCCATCCAACCGTCTCTCAACGCCATTCTCATCCCTCCTC
  ACTTGAGGTCCCCAGCGGGCACTACCACCCTCACCCCGTCCTCACAAGCTTTAACTGAGACTCCGACCAG
  CTCAGACTGGCAGTCGACCGATGCCACACCTACCCTCACTAACTCCTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208698 representing NM_019884
  Red=Cloning site Green=Tags(s)
  
  MSGGGPSGGGPGGSGRARTSSFAEPGGGGGGGGGGPGGSASGPGGTGGGKASVGAMGGGVGASSSGGGPG
  GSGGGGSGGPGAGTSFPPPGVKLGRDSGKVTTVVATLGQGPERSQEVAYTDIKVIGNGSFGVVYQARLAE
  TRELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKKDELYLNLVLEYVPETVYRVARHFT
  KAKLTIPILYVKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPDTAVLKLCDFGSAKQLVRGEPNVSYI
  CSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPN
  YTEFKFPQIKAHPWTKVFKSRTPPEAIALCSSLLEYTPSSRLSPLEACAHSFFDELRCLGTQLPNNRPLP
  PLFNFSAGELSIQPSLNAILIPPHLRSPAGTTTLTPSSQALTETPTSSDWQSTDATPTLTNSS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019884
• ORF Size: 1449 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019884.3
• RefSeq Size: 2200 bp
• RefSeq ORF: 1452 bp
• Locus ID: 2931
• UniProt ID: P49840
• Cytogenetics: 19q13.2
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway
• Gene Summary: This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease. [provided by RefSeq, Oct 2011]