RPL3L (NM_005061) Human Tagged ORF Clone

CAT#: RG208365

RPL3L (tGFP-tagged) - Human ribosomal protein L3-like (RPL3L)

Product Images

Circular map for RG208365

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RPL3L
• Synonyms: CMD2D
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208365 representing NM_005061
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCACCGGAAGTTTTCCGCCCCTCGGCACGGACACCTGGGCTTCCTGCCCCATAAGAGGAGCCACC
  GGCACCGGGGCAAGGTGAAGACGTGGCCGCGGGATGACCCCAGCCAGCCCGTGCACCTCACGGCCTTCCT
  GGGCTACAAGGCGGGCATGACCCACACCCTGCGGGAGGTGCACCGGCCGGGGCTCAAAATTTCCAAACGG
  GAGGAGGTGGAGGCGGTGACAATTGTAGAAACGCCGCCCCTAGTGGTGGTGGGCGTGGTGGGCTACGTGG
  CCACCCCTCGAGGTCTCCGGAGCTTCAAGACCATCTTTGCAGAACACCTCAGTGATGAGTGCCGGCGCCG
  ATTCTACAAGGACTGGCACAAGAGCAAGAAGAAAGCCTTCACCAAGGCCTGCAAGAGGTGGCGGGACACA
  GACGGGAAAAAGCAGCTACAGAAGGACTTCGCCGCCATGAAGAAGTACTGCAAGGTCATTCGGGTCATTG
  TCCACACTCAGATGAAACTGCTGCCCTTCCGGCAGAAGAAGGCCCACATCATGGAGATCCAGCTGAACGG
  TGGCACGGTGGCCGAGAAGGTGGCCTGGGCCCAGGCCCGGCTGGAGAAGCAGGTGCCCGTGCACAGCGTG
  TTCAGCCAGAGTGAGGTCATTGATGTCATTGCTGTCACCAAGGGTCGAGGCGTCAAAGGGGTCACAAGCC
  GCTGGCATACCAAGAAGCTGCCGCGGAAGACCCATAAGGGCCTGCGCAAGGTGGCCTGCATTGGCGCCTG
  GCACCCCGCCCGCGTGGGCTGCTCCATTGCTCGGGCCGGGCAGAAGGGCTATCACCACCGCACGGAGCTC
  AACAAGAAGATCTTCCGCATCGGCAGGGGCCCGCACATGGAGGACGGGAAGCTGGTGAAGAACAATGCAT
  CCACCAGCTACGACGTGACTGCCAAGTCCATCACACCGCTGGGTGGCTTCCCCCACTACGGGGAAGTGAA
  CAACGACTTCGTCATGCTGAAGGGTTGTATTGCTGGTACCAAGAAGCGGGTCATTACGCTGAGAAAGTCC
  CTCCTGGTGCATCACAGTCGCCAAGCCGTGGAGAATATTGAGCTCAAGTTCATTGACACCACCTCCAAGT
  TCGGCCATGGCCGCTTCCAGACAGCCCAAGAGAAGAGGGCCTTCATGGGCCCCCAAAAGAAGCATCTGGA
  GAAGGAAACGCCGGAGACCTCGGGAGACTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208365 representing NM_005061
  Red=Cloning site Green=Tags(s)
  
  MSHRKFSAPRHGHLGFLPHKRSHRHRGKVKTWPRDDPSQPVHLTAFLGYKAGMTHTLREVHRPGLKISKR
  EEVEAVTIVETPPLVVVGVVGYVATPRGLRSFKTIFAEHLSDECRRRFYKDWHKSKKKAFTKACKRWRDT
  DGKKQLQKDFAAMKKYCKVIRVIVHTQMKLLPFRQKKAHIMEIQLNGGTVAEKVAWAQARLEKQVPVHSV
  FSQSEVIDVIAVTKGRGVKGVTSRWHTKKLPRKTHKGLRKVACIGAWHPARVGCSIARAGQKGYHHRTEL
  NKKIFRIGRGPHMEDGKLVKNNASTSYDVTAKSITPLGGFPHYGEVNNDFVMLKGCIAGTKKRVITLRKS
  LLVHHSRQAVENIELKFIDTTSKFGHGRFQTAQEKRAFMGPQKKHLEKETPETSGDL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005061
• ORF Size: 1221 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005061.3
• RefSeq Size: 1547 bp
• RefSeq ORF: 1224 bp
• Locus ID: 6123
• UniProt ID: Q92901
• Cytogenetics: 16p13.3
• Protein Pathways: Ribosome
• Gene Summary: This gene encodes a protein that shares sequence similarity with ribosomal protein L3. The protein belongs to the L3P family of ribosomal proteins. Unlike the ubiquitous expression of ribosomal protein genes, this gene has a tissue-specific pattern of expression, with the highest levels of expression in skeletal muscle and heart. It is not currently known whether the encoded protein is a functional ribosomal protein or whether it has evolved a function that is independent of the ribosome. [provided by RefSeq, Jul 2008]