SENP2 (NM_021627) Human Tagged ORF Clone

CAT#: RG208109

SENP2 (tGFP-tagged) - Human SUMO1/sentrin/SMT3 specific peptidase 2 (SENP2)

Product Images

Circular map for RG208109

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SENP2
• Synonyms: AXAM2; SMT3IP2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208109 representing NM_021627
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACAGATGGCTGGTTAGGATTCTCGGCACCATTTTCCGTTTCTGCGACCGGTCGGTGCCCCCTGCCC
  GGGCCCTCCTGAAGAGGCGGCGCTCAGACAGCACTCTGTTTTCTACAGTGGACACTGATGAAATACCAGC
  CAAAAGACCAAGATTAGATTGCTTTATTCACCAAGTGAAAAACAGTCTCTACAATGCTGCCAGCTTATTT
  GGATTCCCATTCCAGCTGACCACAAAGCCCATGGTAACTTCTGCTTGTAATGGAACACGGAATGTGGCCC
  CTTCAGGAGAGGTATTTTCGAACTCTTCATCTTGTGAACTGACAGGTTCTGGATCCTGGAACAACATGCT
  GAAACTGGGTAATAAATCTCCTAATGGAATAAGTGACTATCCAAAGATCAGAGTGACAGTTACCCGAGAT
  CAGCCACGCAGAGTCCTGCCTTCCTTTGGTTTTACTTTGAACTCAGAAGGCTGTAATAGAAGACCAGGTG
  GCCGTCGCCATAGCAAAGGTAATCCAGAGAGTTCTTTAATGTGGAAACCTCAGGAACAGGCTGTAACAGA
  GATGATTTCTGAAGAGAGTGGCAAGGGTCTGAGGCGTCCCCATTGTACTGTGGAGGAGGGTGTTCAAAAA
  GAGGAAAGAGAGAAGTACCGAAAGTTATTGGAACGACTTAAAGAAAGTGGTCATGGAAACTCTGTCTGTC
  CTGTAACTTCAAATTATCACAGTTCTCAAAGAAGTCAGATGGACACATTAAAGACCAAAGGCTGGGGGGA
  AGAGCAAAATCACGGAGTCAAAACAACTCAGTTTGTTCCAAAACAATATAGACTTGTTGAAACAAGGGGA
  CCTCTATGTTCATTGAGAAGTGAAAAGAGGTGTTCAAAGGGGAAAATTACTGATACAGAGACGATGGTCG
  GAATCAGATTTGAAAATGAAAGTAGGAGGGGATACCAACTGGAGCCTGACCTATCAGAAGAAGTGTCGGC
  CCGACTCCGCCTGGGCAGTGGAAGCAATGGCTTACTCAGGAGGAAAGTGTCAATAATTGAGACAAAGGAA
  AAGAATTGCTCAGGCAAAGAGAGGGACAGAAGAACGGACGATCTCCTTGAACTTACAGAGGACATGGAAA
  AGGAAATCAGTAATGCCCTAGGCCATGGCCCACAGGATGAAATCCTAAGTAGTGCTTTCAAATTGCGAAT
  TACTCGAGGAGATATTCAGACATTAAAGAACTATCACTGGCTCAATGATGAAGTCATTAATTTTTACATG
  AATCTTCTGGTGGAAAGAAATAAAAAGCAAGGCTATCCAGCACTTCATGTATTCAGTACTTTCTTCTATC
  CTAAATTAAAGTCTGGGGGTTACCAAGCAGTGAAACGATGGACCAAAGGGGTAAATCTCTTTGAACAAGA
  AATTATTCTGGTGCCTATTCATCGGAAGGTACATTGGAGCCTGGTGGTGATTGACCTAAGAAAAAAGTGT
  CTTAAATATCTGGATTCTATGGGACAAAAGGGCCACAGGATCTGTGAGATTCTCCTTCAGTATTTACAGG
  ATGAAAGTAAGACCAAAAGAAATAGTGATCTGAATCTTTTAGAGTGGACCCATCACAGCATGAAACCACA
  CGAGATTCCTCAACAGCTGAATGGGAGTGATTGTGGAATGTTTACTTGTAAATATGCAGATTATATTTCT
  AGGGACAAACCTATCACATTTACTCAGCACCAGATGCCTCTCTTCCGGAAGAAGATGGTGTGGGAAATCC
  TTCATCAGCAGTTGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208109 representing NM_021627
  Red=Cloning site Green=Tags(s)
  
  MYRWLVRILGTIFRFCDRSVPPARALLKRRRSDSTLFSTVDTDEIPAKRPRLDCFIHQVKNSLYNAASLF
  GFPFQLTTKPMVTSACNGTRNVAPSGEVFSNSSSCELTGSGSWNNMLKLGNKSPNGISDYPKIRVTVTRD
  QPRRVLPSFGFTLNSEGCNRRPGGRRHSKGNPESSLMWKPQEQAVTEMISEESGKGLRRPHCTVEEGVQK
  EEREKYRKLLERLKESGHGNSVCPVTSNYHSSQRSQMDTLKTKGWGEEQNHGVKTTQFVPKQYRLVETRG
  PLCSLRSEKRCSKGKITDTETMVGIRFENESRRGYQLEPDLSEEVSARLRLGSGSNGLLRRKVSIIETKE
  KNCSGKERDRRTDDLLELTEDMEKEISNALGHGPQDEILSSAFKLRITRGDIQTLKNYHWLNDEVINFYM
  NLLVERNKKQGYPALHVFSTFFYPKLKSGGYQAVKRWTKGVNLFEQEIILVPIHRKVHWSLVVIDLRKKC
  LKYLDSMGQKGHRICEILLQYLQDESKTKRNSDLNLLEWTHHSMKPHEIPQQLNGSDCGMFTCKYADYIS
  RDKPITFTQHQMPLFRKKMVWEILHQQLL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021627
• ORF Size: 1767 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021627.3
• RefSeq Size: 3207 bp
• RefSeq ORF: 1770 bp
• Locus ID: 59343
• UniProt ID: Q9HC62
• Cytogenetics: 3q27.2
• Domains: Peptidase_C48
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Wnt signaling pathway
• Gene Summary: SUMO1 (UBL1; MIM 601912) is a small ubiquitin-like protein that can be covalently conjugated to other proteins. SENP2 is one of a group of enzymes that process newly synthesized SUMO1 into the conjugatable form and catalyze the deconjugation of SUMO1-containing species.[supplied by OMIM, Apr 2004]