PRKACG (NM_002732) Human Tagged ORF Clone

CAT#: RG207946

PRKACG (tGFP-tagged) - Human protein kinase, cAMP-dependent, catalytic, gamma (PRKACG)

Product Images

Circular map for RG207946

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PRKACG
• Synonyms: BDPLT19; KAPG; PKACg
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207946 representing NM_002732
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAACGCCCCCGCCAAGAAGGACACCGAGCAGGAGGAGAGCGTGAACGAGTTCCTAGCCAAAGCCA
  GAGGAGATTTCCTCTACAGATGGGGAAACCCCGCTCAAAACACCGCCAGCTCGGATCAGTTCGAACGGCT
  CAGGACGCTGGGCATGGGCTCCTTCGGGCGGGTGATGCTGGTGAGGCACCAGGAGACCGGCGGCCACTAC
  GCCATGAAGATCCTCAACAAGCAGAAGGTGGTGAAGATGAAGCAGGTCGAGCACATACTGAACGAGAAGC
  GCATCCTGCAGGCGATCGACTTTCCGTTCCTCGTCAAGCTCCAGTTCTCCTTTAAGGACAACTCCTACCT
  GTACCTGGTGATGGAGTACGTGCCGGGTGGGGAGATGTTCTCCCGCCTACAGCGCGTCGGAAGGTTTAGC
  GAGCCCCATGCCTGTTTCTATGCCGCCCAGGTCGTCCTGGCCGTCCAGTACCTACACTCGCTCGACCTCA
  TCCACCGCGACCTGAAGCCCGAGAATCTCCTCATCGACCAGCAGGGCTACCTGCAGGTGACGGACTTCGG
  TTTCGCCAAGCGCGTGAAGGGCCGCACTTGGACCTTGTGCGGGACCCCAGAGTACCTGGCCCCCGAGATC
  ATCCTGAGCAAAGGCTACAACAAGGCCGTGGACTGGTGGGCCCTAGGGGTGCTCATCTATGAGATGGCCG
  TGGGCTTCCCACCCTTCTACGCCGACCAGCCCATCCAGATCTACGAGAAGATCGTCTCTGGGAGGGTGCG
  GTTTCCCTCCAAACTCAGCTCTGACCTCAAGCATCTGCTGCGGAGCCTGCTGCAGGTGGACCTCACCAAG
  CGCTTCGGAAACCTCAGGAACGGGGTTGGCGACATCAAGAACCACAAGTGGTTCGCCACAACCAGCTGGA
  TCGCCATCTATGAGAAGAAGGTGGAAGCTCCCTTCATCCCGAAGTACACAGGCCCTGGGGATGCCAGTAA
  CTTTGACGACTACGAGGAGGAAGAGCTCCGGATCTCCATCAATGAGAAGTGTCCCAAGGAGTTTTCTGAG
  TTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207946 representing NM_002732
  Red=Cloning site Green=Tags(s)
  
  MGNAPAKKDTEQEESVNEFLAKARGDFLYRWGNPAQNTASSDQFERLRTLGMGSFGRVMLVRHQETGGHY
  AMKILNKQKVVKMKQVEHILNEKRILQAIDFPFLVKLQFSFKDNSYLYLVMEYVPGGEMFSRLQRVGRFS
  EPHACFYAAQVVLAVQYLHSLDLIHRDLKPENLLIDQQGYLQVTDFGFAKRVKGRTWTLCGTPEYLAPEI
  ILSKGYNKAVDWWALGVLIYEMAVGFPPFYADQPIQIYEKIVSGRVRFPSKLSSDLKHLLRSLLQVDLTK
  RFGNLRNGVGDIKNHKWFATTSWIAIYEKKVEAPFIPKYTGPGDASNFDDYEEEELRISINEKCPKEFSE
  F
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002732
• ORF Size: 1053 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002732.2, NP_002723.2
• RefSeq Size: 1560 bp
• RefSeq ORF: 1056 bp
• Locus ID: 5568
• UniProt ID: P22612
• Cytogenetics: 9q21.11
• Domains: pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Apoptosis, Calcium signaling pathway, Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Hedgehog signaling pathway, Insulin signaling pathway, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Olfactory transduction, Oocyte meiosis, Prion diseases, Progesterone-mediated oocyte maturation, Taste transduction, Vascular smooth muscle contraction, Vibrio cholerae infection, Wnt signaling pathway
• Gene Summary: Cyclic AMP-dependent protein kinase (PKA) consists of two catalytic subunits and a regulatory subunit dimer. This gene encodes the gamma form of its catalytic subunit. The gene is intronless and is thought to be a retrotransposon derived from the gene for the alpha form of the PKA catalytic subunit. [provided by RefSeq, Jul 2008]