CROT (NM_021151) Human Tagged ORF Clone

CAT#: RG207888

CROT (tGFP-tagged) - Human carnitine O-octanoyltransferase (CROT), transcript variant 2

Product Images

Circular map for RG207888

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CROT
• Synonyms: COT
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207888 representing NM_021151
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAATCAATTGGCTAAATCAACTGAAGAACGAACATTTCAGTACCAGGATTCTCTTCCATCACTGC
  CTGTTCCTTCACTTGAAGAATCATTAAAAAAATACCTTGAATCAGTGAAACCATTTGCAAATCAAGAAGA
  ATATAAGAAAACTGAAGAAATAGTTCAAAAATTTCAAAGTGGGATTGGAGAAAAATTGCACCAGAAATTG
  CTTGAAAGAGCAAAAGGAAAAAGAAATTGGCTGGAAGAGTGGTGGCTGAATGTTGCCTATCTGGATGTTC
  GTATACCATCACAATTGAATGTCAACTTTGCGGGTCCTGCAGCTCATTTTGAACACTACTGGCCTCCAAA
  GGAAGGGACTCAATTAGAAAGAGGAAGTATAACTCTTTGGCATAACTTGAACTACTGGCAGCTATTAAGA
  AAAGAAAAAGTGCCTGTTCATAAAGTTGGAAATACTCCTCTAGATATGAATCAATTCCGAATGCTATTTT
  CTACCTGCAAGGTTCCAGGAATTACTAGAGACTCCATTATGAATTATTTTAGGACTGAGAGTGAAGGGCG
  TTCCCCAAACCACATTGTAGTGCTGTGTCGAGGCCGAGCTTTTGTCTTTGATGTAATACATGAAGGATGT
  TTGGTCACCCCGCCAGAGCTTCTCAGACAACTGACATATATCCACAAGAAGTGCCATAGTGAACCTGATG
  GACCTGGGATTGCAGCATTAACTAGTGAGGAGCGAACTCGATGGGCTAAGGCACGAGAATATCTGATTGG
  TCTTGATCCAGAGAACTTGGCTTTGTTAGAAAAAATTCAGAGTAGTTTACTGGTATATTCCATGGAGGAT
  AGCAGTCCACATGTAACACCAGAGGATTATTCTGAGATTATTGCAGCCATCCTTATTGGAGATCCAACAG
  TACGCTGGGGTGACAAATCCTATAACTTGATTTCCTTTTCTAATGGAGTATTTGGCTGTAATTGTGATCA
  TGCTCCTTTTGATGCAATGATTATGGTGAACATCAGTTATTATGTGGATGAGAAAATTTTTCAGAATGAA
  GGAAGATGGAAGGGTTCAGAGAAGGTACGAGATATACCACTTCCAGAAGAGCTCATTTTCATTGTGGATG
  AGAAAGTTTTAAATGACATCAACCAAGCTAAAGCCCAGTATCTCAGGGAGGCATCTGATCTACAGATTGC
  GGCTTATGCCTTTACATCTTTTGGCAAAAAGCTAACCAAGAACAAGATGCTTCACCCGGATACGTTTATT
  CAGCTTGCACTTCAGCTGGCCTATTACAGACTTCATGGACACCCTGGTTGTTGCTATGAAACAGCTATGA
  CAAGACATTTTTATCATGGCCGTACAGAGACTATGCGATCATGCACAGTTGAAGCAGTGAGGTGGTGCCA
  GTCCATGCAGGATCCTTCTGTCAATCTTCGTGAGCGGCAGCAAAAGATGTTACAAGCTTTTGCAAAGCAT
  AATAAAATGATGAAAGATTGTTCAGCTGGAAAAGGATTTGATCGTCACCTTTTAGGTCTCTTACTCATAG
  CAAAAGAGGAAGGTCTTCCTGTTCCAGAACTCTTTACGGACCCACTTTTTTCCAAAAGCGGAGGAGGTGG
  AAATTTTGTTCTCTCAACAAGTCTGGTTGGCTATTTACGAGTCCAGGGAGTGGTAGTTCCCATGGTACAC
  AATGGTTATGGATTTTTCTACCATATCAGAGATGACAGGTTTGTTGTGGCCTGTTCAGCCTGGAAATCCT
  GTCCCGAGACTGATGCGGAAAAGCTAGTTCAGCTGACTTTTTGTGCTTTTCATGATATGATACAGCTGAT
  GAACTCTACTCATCTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207888 representing NM_021151
  Red=Cloning site Green=Tags(s)
  
  MENQLAKSTEERTFQYQDSLPSLPVPSLEESLKKYLESVKPFANQEEYKKTEEIVQKFQSGIGEKLHQKL
  LERAKGKRNWLEEWWLNVAYLDVRIPSQLNVNFAGPAAHFEHYWPPKEGTQLERGSITLWHNLNYWQLLR
  KEKVPVHKVGNTPLDMNQFRMLFSTCKVPGITRDSIMNYFRTESEGRSPNHIVVLCRGRAFVFDVIHEGC
  LVTPPELLRQLTYIHKKCHSEPDGPGIAALTSEERTRWAKAREYLIGLDPENLALLEKIQSSLLVYSMED
  SSPHVTPEDYSEIIAAILIGDPTVRWGDKSYNLISFSNGVFGCNCDHAPFDAMIMVNISYYVDEKIFQNE
  GRWKGSEKVRDIPLPEELIFIVDEKVLNDINQAKAQYLREASDLQIAAYAFTSFGKKLTKNKMLHPDTFI
  QLALQLAYYRLHGHPGCCYETAMTRHFYHGRTETMRSCTVEAVRWCQSMQDPSVNLRERQQKMLQAFAKH
  NKMMKDCSAGKGFDRHLLGLLLIAKEEGLPVPELFTDPLFSKSGGGGNFVLSTSLVGYLRVQGVVVPMVH
  NGYGFFYHIRDDRFVVACSAWKSCPETDAEKLVQLTFCAFHDMIQLMNSTHL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021151
• ORF Size: 1836 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021151.4
• RefSeq Size: 3211 bp
• RefSeq ORF: 1839 bp
• Locus ID: 54677
• UniProt ID: Q9UKG9
• Cytogenetics: 7q21.12
• Domains: Carn_acyltransf
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the carnitine/choline acetyltransferase family. The encoded protein converts 4,8-dimethylnonanoyl-CoA to its corresponding carnitine ester. This transesterification occurs in the peroxisome and is necessary for transport of medium- and long- chain acyl-CoA molecules out of the peroxisome to the cytosol and mitochondria. The protein thus plays a role in lipid metabolism and fatty acid beta-oxidation. Alternatively spliced transcript variants have been described.[provided by RefSeq, Jan 2009]