PHF17 (JADE1) (NM_024900) Human Tagged ORF Clone

CAT#: RG207135

PHF17 (tGFP-tagged) - Human PHD finger protein 17 (PHF17), transcript variant S

Product Images

Circular map for RG207135

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PHF17
• Synonyms: PHF17
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207135 representing NM_024900
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAACGAGGTCGCCTTCCCAGCAGCAGTGAGGATTCTGACGACAATGGCAGCCTGTCAACTACTTGGT
  CCCAGAATTCCCGATCCCAGCATAGGAGAAGCTCCTGCTCCAGACATGAAGATCGAAAGCCTTCAGAGGT
  GTTTAGGACAGACCTGATCACTGCCATGAAGTTGCATGACTCCTACCAGCTGAATCCGGATGAGTACTAT
  GTGTTGGCAGATCCCTGGAGACAGGAATGGGAGAAAGGGGTCCAGGTGCCTGTGAGCCCGGGGACCATCC
  CTCAGCCTGTGGCCAGGGTTGTGTCTGAAGAGAAATCCCTCATGTTCATCAGGCCCAAGAAGTACATCGT
  GTCATCAGGCTCTGAGCCTCCCGAGTTGGGCTATGTGGACATCCGGACGCTGGCTGACAGCGTGTGTCGC
  TATGACCTCAATGACATGGATGCTGCATGGCTGGAACTGACCAATGAAGAATTTAAGGAGATGGGAATGC
  CTGAACTAGATGAATACACCATGGAGAGGGTCCTAGAGGAATTTGAGCAGCGATGCTACGACAATATGAA
  TCATGCCATAGAGACTGAGGAAGGCCTGGGGATCGAATATGATGAAGATGTTGTCTGTGATGTCTGCCAG
  TCTCCTGATGGTGAGGACGGCAATGAGATGGTGTTCTGTGACAAATGCAACATCTGTGTGCACCAGGCCT
  GTTATGGAATCCTCAAGGTACCAGAGGGCAGCTGGCTGTGCCGGACATGTGCCCTGGGGGTTCAGCCAAA
  ATGTCTGCTGTGTCCGAAGAAGGGTGGAGCTATGAAGCCCACCCGTAGCGGAACCAAGTGGGTCCACGTT
  AGCTGTGCTCTGTGGATCCCTGAGGTGAGCATTGGCAGCCCAGAGAAGATGGAGCCCATCACCAAGGTGT
  CACACATTCCCAGCAGCCGGTGGGCGCTAGTGTGCAGCCTCTGCAATGAGAAGTTTGGGGCCTCTATACA
  GTGCTCTGTGAAGAACTGCCGCACAGCCTTCCATGTGACCTGTGCTTTTGACCGGGGCCTGGAGATGAAG
  ACCATCTTAGCAGAGAATGATGAAGTCAAGTTCAAGTCCTATTGCCCAAAGCACAGCTCACATAGGAAAC
  CCGAGGAGAGTCTTGGCAAGGGGGCTGCACAGGAGAATGGGGCCCCTGAGTGTTCCCCCCGGAATCCGCT
  GGAGCCCTTTGCCAGCCTTGAGCAGAACCGGGAGGAGGCCCACCGGGTGAGTGTCCGTAAGCAGAAGCTG
  CAGCAGTTGGAGGATGAGTTCTACACCTTCGTCAACCTGCTGGATGTTGCCAGGGCTCTGCGGCTGCCTG
  AGGAAGTAGTGGATTTCCTGTACCAGTACTGGAAGTTGAAGAGGAAGGTCAACTTCAACAAGCCCCTGAT
  CACCCCAAAGAAAGATGAAGAGGACAATCTAGCCAAGCGGGAGCAGGATGTCTTATTTAGGAGGCTGCAG
  CTGTTCACGCACCTGCGGCAGGACCTGGAGAGGGTAATGATTGACACTGACACCTTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207135 representing NM_024900
  Red=Cloning site Green=Tags(s)
  
  MKRGRLPSSSEDSDDNGSLSTTWSQNSRSQHRRSSCSRHEDRKPSEVFRTDLITAMKLHDSYQLNPDEYY
  VLADPWRQEWEKGVQVPVSPGTIPQPVARVVSEEKSLMFIRPKKYIVSSGSEPPELGYVDIRTLADSVCR
  YDLNDMDAAWLELTNEEFKEMGMPELDEYTMERVLEEFEQRCYDNMNHAIETEEGLGIEYDEDVVCDVCQ
  SPDGEDGNEMVFCDKCNICVHQACYGILKVPEGSWLCRTCALGVQPKCLLCPKKGGAMKPTRSGTKWVHV
  SCALWIPEVSIGSPEKMEPITKVSHIPSSRWALVCSLCNEKFGASIQCSVKNCRTAFHVTCAFDRGLEMK
  TILAENDEVKFKSYCPKHSSHRKPEESLGKGAAQENGAPECSPRNPLEPFASLEQNREEAHRVSVRKQKL
  QQLEDEFYTFVNLLDVARALRLPEEVVDFLYQYWKLKRKVNFNKPLITPKKDEEDNLAKREQDVLFRRLQ
  LFTHLRQDLERVMIDTDTL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024900
• ORF Size: 1527 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024900.5
• RefSeq Size: 4190 bp
• RefSeq ORF: 1530 bp
• Locus ID: 79960
• UniProt ID: Q6IE81
• Cytogenetics: 4q28.2
• Domains: PHD
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Transcriptional coactivator, it may also promote acetylation of nucleosomal histone H4 by KAT5. Promotes apoptosis. May act as a renal tumor suppressor. Negatively regulates canonical Wnt signaling; at least in part, cooperates with NPHP4 in this function.[UniProtKB/Swiss-Prot Function]