ALS (IGFALS) (NM_004970) Human Tagged ORF Clone

CAT#: RG206542

IGFALS (tGFP-tagged) - Human insulin-like growth factor binding protein, acid labile subunit (IGFALS), transcript variant 2

Product Images

Circular map for RG206542

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ALS
• Synonyms: ACLSD; ALS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206542 representing NM_004970
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTGAGGAAAGGAGGCCTGGCCCTGGCGCTGCTGCTGCTGTCCTGGGTGGCACTGGGCCCCCGCA
  GCCTGGAGGGAGCAGACCCCGGAACGCCGGGGGAAGCCGAGGGCCCAGCGTGCCCGGCCGCCTGTGTCTG
  CAGCTACGATGACGACGCGGATGAGCTCAGCGTCTTCTGCAGCTCCAGGAACCTCACGCGCCTGCCTGAT
  GGAGTCCCGGGCGGCACCCAAGCCCTGTGGCTGGACGGCAACAACCTCTCGTCCGTCCCCCCGGCAGCCT
  TCCAGAACCTCTCCAGCCTGGGCTTCCTCAACCTGCAGGGCGGCCAGCTGGGCAGCCTGGAGCCACAGGC
  GCTGCTGGGCCTAGAGAACCTGTGCCACCTGCACCTGGAGCGGAACCAGCTGCGCAGCCTGGCACTCGGC
  ACGTTTGCACACACGCCCGCGCTGGCCTCGCTCGGCCTCAGCAACAACCGTCTGAGCAGGCTGGAGGACG
  GGCTCTTCGAGGGCCTCGGCAGCCTCTGGGACCTCAACCTCGGCTGGAATAGCCTGGCGGTGCTCCCCGA
  TGCGGCGTTCCGCGGCCTGGGCAGCCTGCGCGAGCTGGTGCTGGCGGGCAACAGGCTGGCCTACCTGCAG
  CCCGCGCTCTTCAGCGGCCTGGCCGAGCTCCGGGAGCTGGACCTGAGCAGGAACGCGCTGCGGGCCATCA
  AGGCAAACGTGTTCGTGCAGCTGCCCCGGCTCCAGAAACTCTACCTGGACCGCAACCTCATCGCTGCCGT
  GGCCCCGGGCGCCTTCCTGGGCCTGAAGGCGCTGCGATGGCTGGACCTGTCCCACAACCGCGTGGCTGGC
  CTCCTGGAGGACACGTTCCCCGGTCTGCTGGGCCTGCGTGTGCTGCGGCTGTCCCACAACGCCATCGCCA
  GCCTGCGGCCCCGCACCTTCAAGGACCTGCACTTCCTGGAGGAGCTGCAGCTGGGCCACAACCGCATCCG
  GCAGCTGGCTGAGCGCAGCTTTGAGGGCCTGGGGCAGCTTGAGGTGCTCACGCTAGACCACAACCAGCTC
  CAGGAGGTCAAGGCGGGCGCTTTCCTCGGCCTCACCAACATGGCGGTCATGAACCTCTCTGGGAACTGTC
  TCCGGAACCTTCCGGAGCAGGTGTTCCGGGGCCTGGGCAAGCTGCACAGCCTGCACCTGGAGGGCAGCTG
  CCTGGGACGCATCCGCCCGCACACCTTCACCGGCCTCTCGGGGCTCCGCCGACTCTTCCTCAAGGACAAC
  GGCCTCGTGGGCATTGAGGAGCAGAGCCTGTGGGGGCTGGCGGAGCTGCTGGAGCTCGACCTGACCTCCA
  ACCAGCTCACGCACCTGCCCCACCGCCTCTTCCAGGGCCTGGGCAAGCTGGAGTACCTGCTGCTCTCCCG
  CAACCGCCTGGCAGAGCTGCCGGCGGACGCCCTGGGCCCCCTGCAGCGGGCCTTCTGGCTGGACGTCTCG
  CACAACCGCCTGGAGGCATTGCCCAACAGCCTCTTGGCACCACTGGGGCGGCTGCGCTACCTCAGCCTCA
  GGAACAACTCACTGCGGACCTTCACGCCGCAGCCCCCGGGCCTGGAGCGCCTGTGGCTGGAGGGTAACCC
  CTGGGACTGTGGCTGCCCTCTCAAGGCGCTGCGGGACTTCGCCCTGCAGAACCCCAGTGCTGTGCCCCGC
  TTCGTCCAGGCCATCTGTGAGGGGGACGATTGCCAGCCGCCCGCGTACACCTACAACAACATCACCTGTG
  CCAGCCCGCCCGAGGTCGTGGGGCTCGACCTGCGGGACCTCAGCGAGGCCCACTTTGCTCCCTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206542 representing NM_004970
  Red=Cloning site Green=Tags(s)
  
  MALRKGGLALALLLLSWVALGPRSLEGADPGTPGEAEGPACPAACVCSYDDDADELSVFCSSRNLTRLPD
  GVPGGTQALWLDGNNLSSVPPAAFQNLSSLGFLNLQGGQLGSLEPQALLGLENLCHLHLERNQLRSLALG
  TFAHTPALASLGLSNNRLSRLEDGLFEGLGSLWDLNLGWNSLAVLPDAAFRGLGSLRELVLAGNRLAYLQ
  PALFSGLAELRELDLSRNALRAIKANVFVQLPRLQKLYLDRNLIAAVAPGAFLGLKALRWLDLSHNRVAG
  LLEDTFPGLLGLRVLRLSHNAIASLRPRTFKDLHFLEELQLGHNRIRQLAERSFEGLGQLEVLTLDHNQL
  QEVKAGAFLGLTNMAVMNLSGNCLRNLPEQVFRGLGKLHSLHLEGSCLGRIRPHTFTGLSGLRRLFLKDN
  GLVGIEEQSLWGLAELLELDLTSNQLTHLPHRLFQGLGKLEYLLLSRNRLAELPADALGPLQRAFWLDVS
  HNRLEALPNSLLAPLGRLRYLSLRNNSLRTFTPQPPGLERLWLEGNPWDCGCPLKALRDFALQNPSAVPR
  FVQAICEGDDCQPPAYTYNNITCASPPEVVGLDLRDLSEAHFAPC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004970
• ORF Size: 1815 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004970.1, NP_004961.1
• RefSeq Size: 2125 bp
• RefSeq ORF: 1818 bp
• Locus ID: 3483
• UniProt ID: P35858
• Cytogenetics: 16p13.3
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: The protein encoded by this gene is a serum protein that binds insulin-like growth factors, increasing their half-life and their vascular localization. Production of the encoded protein, which contains twenty leucine-rich repeats, is stimulated by growth hormone. Defects in this gene are a cause of acid-labile subunit deficiency, which maifests itself in a delayed and slow puberty. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]