PKD2L1 (NM_016112) Human Tagged ORF Clone

CAT#: RG206480

PKD2L1 (tGFP-tagged) - Human polycystic kidney disease 2-like 1 (PKD2L1)

Product Images

Circular map for RG206480

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PKD2L1
• Synonyms: PCL; PKD2L; PKDL; TRPP3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206480 representing NM_016112
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGCTGTGGGAAGTCCTGAGGGGCAGGAGCTGCAAAAGCTGGGGAGTGGAGCCTGGGACAACCCCG
  CCTACAGTGGTCCCCCTTCCCCACACGGGACGCTGAGAGTCTGCACCATCTCCAGCACGGGGCCTCTCCA
  GCCCCAACCCAAGAAGCCTGAAGATGAACCCCAGGAGACGGCATACAGGACCCAGGTGTCCAGCTGCTGC
  CTCCATATCTGTCAAGGCATCAGAGGACTTTGGGGAACAACCCTGACTGAGAACACAGCTGAGAACCGGG
  AACTTTATATCAAGACCACCCTGAGGGAGCTGTTGGTATATATTGTGTTCCTGGTGGACATCTGTCTACT
  GACCTATGGAATGACAAGCTCCAGTGCTTATTACTACACCAAAGTGATGTCTGAGCTCTTCTTACATACT
  CCATCAGACACTGGAGTCTCCTTTCAGGCCATCAGCAGCATGGCGGACTTCTGGGATTTTGCCCAGGGCC
  CACTACTGGACAGTTTGTATTGGACCAAATGGTACAACAACCAGAGCCTGGGCCATGGCTCCCACTCCTT
  CATCTACTATGAGAACATGCTGCTGGGGGTTCCGAGGCTGCGGCAGCTAAAGGTCCGCAATGACTCCTGT
  GTGGTGCATGAAGACTTCCGGGAGGACATTCTGAGCTGCTATGATGTCTACTCTCCAGACAAAGAAGAAC
  AACTCCCCTTTGGGCCCTTCAATGGCACAGCGTGGACATACCACTCGCAGGATGAGTTGGGGGGCTTCTC
  CCACTGGGGCAGGCTCACAAGCTACAGCGGAGGTGGCTACTACCTGGACCTTCCAGGATCCCGACAGGGT
  AGTGCAGAGGCTCTCCGGGCCCTTCAGGAGGGGCTGTGGCTGGACAGGGGCACTCGAGTGGTGTTCATCG
  ACTTCTCAGTCTACAATGCCAATATCAATCTTTTCTGTGTCCTGAGGCTGGTGGTGGAGTTTCCAGCTAC
  AGGAGGTGCCATCCCATCCTGGCAAATCCGCACAGTCAAGCTGATCCGCTATGTCAGCAACTGGGACTTC
  TTTATCGTTGGCTGTGAGGTCATCTTCTGCGTCTTCATCTTCTACTATGTGGTGGAAGAGATCCTGGAGC
  TCCACATTCACCGGCTTCGCTACCTCAGCAGCATCTGGAACATACTGGACCTGGTGGTCATCTTGCTCTC
  CATTGTGGCTGTGGGCTTCCACATATTCCGAACCCTCGAGGTGAATCGGCTCATGGGGAAGCTCCTGCAG
  CAGCCAAACACGTATGCAGACTTTGAGTTCCTCGCCTTCTGGCAGACACAGTACAACAACATGAATGCTG
  TCAACCTCTTCTTCGCCTGGATCAAGATATTCAAGTACATCAGCTTCAACAAAACCATGACCCAGCTCTC
  CTCCACGCTGGCCCGCTGTGCCAAGGACATCCTGGGCTTCGCCGTCATGTTCTTCATTGTTTTCTTCGCC
  TATGCCCAACTCGGCTACCTGCTTTTCGGGACCCAAGTGGAAAACTTTAGCACTTTCATCAAGTGCATTT
  TCACTCAGTTCCGGATAATCCTCGGGGACTTTGACTACAATGCTATCGACAATGCCAACCGCATCCTGGG
  CCCTGCCTACTTTGTCACCTATGTCTTCTTCGTCTTCTTCGTGCTCCTGAACATGTTCCTGGCCATCATC
  AATGACACATATTCAGAGGTCAAGGAGGAGCTGGCTGGACAGAAGGATGAGCTGCAACTTTCTGACCTCC
  TGAAACAGGGCTACAACAAGACCCTACTAAGACTGCGTCTGAGGAAGGAGAGGGTTTCGGATGTGCAGAA
  GGTCCTGCAGGGTGGGGAGCAGGAGATCCAGTTTGAGGATTTCACCAACACCTTAAGGGAACTGGGACAC
  GCAGAGCATGAAATCACTGAGCTCACGGCCACCTTCACCAAGTTTGACAGAGATGGGAATCGTATTCTGG
  ATGAGAAGGAACAGGAAAAAATGCGACAGGACCTGGAGGAAGAGAGGGTGGCCCTCAACACTGAGATTGA
  GAAACTAGGCCGATCTATTGTGAGCAGCCCACAAGGCAAATCGGGTCCAGAGGCTGCCAGAGCAGGAGGC
  TGGGTTTCAGGAGAAGAATTCTACATGCTCACAAGGAGAGTTCTGCAGCTGGAGACTGTCCTGGAAGGAG
  TAGTGTCCCAGATTGATGCTGTAGGCTCAAAGCTGAAAATGCTGGAGAGGAAGGGGTGGCTGGCTCCCTC
  CCCAGGCGTGAAGGAACAAGCTATTTGGAAGCACCCGCAGCCAGCCCCAGCTGTGACCCCAGACCCCTGG
  GGAGTCCAGGGTGGGCAGGAGAGTGAGGTTCCCTATAAAAGAGAAGAGGAAGCCTTAGAGGAGAGGAGAC
  TCTCCCGTGGTGAGATTCCAACGTTGCAGAGGAGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206480 representing NM_016112
  Red=Cloning site Green=Tags(s)
  
  MNAVGSPEGQELQKLGSGAWDNPAYSGPPSPHGTLRVCTISSTGPLQPQPKKPEDEPQETAYRTQVSSCC
  LHICQGIRGLWGTTLTENTAENRELYIKTTLRELLVYIVFLVDICLLTYGMTSSSAYYYTKVMSELFLHT
  PSDTGVSFQAISSMADFWDFAQGPLLDSLYWTKWYNNQSLGHGSHSFIYYENMLLGVPRLRQLKVRNDSC
  VVHEDFREDILSCYDVYSPDKEEQLPFGPFNGTAWTYHSQDELGGFSHWGRLTSYSGGGYYLDLPGSRQG
  SAEALRALQEGLWLDRGTRVVFIDFSVYNANINLFCVLRLVVEFPATGGAIPSWQIRTVKLIRYVSNWDF
  FIVGCEVIFCVFIFYYVVEEILELHIHRLRYLSSIWNILDLVVILLSIVAVGFHIFRTLEVNRLMGKLLQ
  QPNTYADFEFLAFWQTQYNNMNAVNLFFAWIKIFKYISFNKTMTQLSSTLARCAKDILGFAVMFFIVFFA
  YAQLGYLLFGTQVENFSTFIKCIFTQFRIILGDFDYNAIDNANRILGPAYFVTYVFFVFFVLLNMFLAII
  NDTYSEVKEELAGQKDELQLSDLLKQGYNKTLLRLRLRKERVSDVQKVLQGGEQEIQFEDFTNTLRELGH
  AEHEITELTATFTKFDRDGNRILDEKEQEKMRQDLEEERVALNTEIEKLGRSIVSSPQGKSGPEAARAGG
  WVSGEEFYMLTRRVLQLETVLEGVVSQIDAVGSKLKMLERKGWLAPSPGVKEQAIWKHPQPAPAVTPDPW
  GVQGGQESEVPYKREEEALEERRLSRGEIPTLQRS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016112
• ORF Size: 2415 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016112.3
• RefSeq Size: 3060 bp
• RefSeq ORF: 2418 bp
• Locus ID: 9033
• UniProt ID: Q9P0L9
• Cytogenetics: 10q24.31
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• Gene Summary: This gene encodes a member of the polycystin protein family. The encoded protein contains multiple transmembrane domains, and cytoplasmic N- and C-termini. The protein may be an integral membrane protein involved in cell-cell/matrix interactions. This protein functions as a calcium-regulated nonselective cation channel. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]