HIPK1 (NM_181358) Human Tagged ORF Clone

CAT#: RG206176

HIPK1 (tGFP-tagged) - Human homeodomain interacting protein kinase 1 (HIPK1), transcript variant 4

Product Images

Circular map for RG206176

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HIPK1
• Synonyms: Myak; Nbak2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206176 representing NM_181358
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTTTGATGTTTCAGATTCGTTATATTTCACAAACACAAGGCTTGCCAGCTGAATATCTTCTCAGTG
  CCGGAACAAAAACAACCAGGTTTTTCAACAGAGATCCTAATTTGGGGTACCCACTGTGGAGGCTTAAGAC
  ACCTGAAGAACATGAACTGGAGACTGGAATAAAATCAAAAGAAGCTCGGAAGTACATTTTTAATTGCTTA
  GATGACATGGCTCAGGTGAATATGTCTACGGACCTGGAGGGAACAGACATGTTGGCAGAGAAGGCAGACC
  GAAGAGAATACATTGATCTGTTAAAGAAAATGCTCACAATTGATGCAGATAAGAGAATTACCCCTCTAAA
  AACTCTTAACCATCAGTTTGTGACAATGACTCACCTTTTGGATTTTCCACATAGCAATCATGTTAAGTCT
  TGTTTTCAGAACATGGAGATCTGCAAGCGGAGGGTTCACATGTATGATACAGTGAGTCAGATCAAGAGTC
  CCTTCACTACACATGTTGCCCCAAATACAAGCACAAATCTAACCATGAGCTTCAGCAATCAGCTCAATAC
  AGTGCACAATCAGGCCAGTGTTCTAGCTTCCAGTTCTACTGCAGCAGCTGCTACTCTTTCTCTGGCTAAT
  TCAGATGTCTCACTACTAAACTACCAGTCAGCTTTGTACCCATCATCTGCTGCACCAGTTCCTGGAGTTG
  CCCAGCAGGGTGTTTCCTTGCAGCCTGGAACCACCCAGATTTGCACTCAGACAGATCCATTCCAACAGAC
  ATTTATAGTATGTCCACCTGCGTTTCAAACTGGACTACAAGCAACAACAAAGCATTCTGGATTCCCTGTG
  AGGATGGATAATGCTGTACCAATTGTACCCCAGGCACCAGCTGCTCAGCCACTACAGATTCAGTCAGGAG
  TTCTCACGCAGGGAAGCTGTACACCACTAATGGTAGCAACTCTCCACCCTCAAGTAGCCACCATCACACC
  GCAGTATGCGGTGCCCTTTACTCTGAGCTGCGCAGCCGGCCGGCCGGCGCTGGTTGAACAGACTGCCGCT
  GTACTGCAGGCGTGGCCTGGAGGGACTCAGCAAATTCTCCTGCCTTCAACTTGGCAACAGTTGCCTGGGG
  TAGCTCTACACAACTCTGTCCAGCCCACAGCAATGATTCCAGAGGCCATGGGGAGTGGACAGCAGCTAGC
  TGACTGGAGGAATGCCCACTCTCATGGCAACCAGTACAGCACTATCATGCAGCAGCCATCCTTGCTGACT
  AACCATGTGACATTGGCCACTGCTCAGCCTCTGAATGTTGGTGTTGCCCATGTTGTCAGACAACAACAAT
  CCAGTTCCCTCCCTTCGAAGAAGAATAAGCAGTCAGCTCCAGTCTCTTCCAAGTCCTCTCTAGATGTTCT
  GCCTTCCCAAGTCTATTCTCTGGTTGGGAGCAGTCCCCTCCGCACCACATCTTCTTATAATTCCTTGGTC
  CCTGTCCAAGATCAGCATCAGCCCATCATCATTCCAGATACTCCCAGCCCTCCTGTGAGTGTCATCACTA
  TCCGAAGTGACACTGATGAGGAAGAGGACAACAAATACAAGCCCAGTAGCTCTGGACTGAAGCCAAGGTC
  TAATGTCATCAGTTATGTCACTGTCAATGATTCTCCTGACTCTGACTCTTCTTTGAGCAGCCCTTATTCC
  ACTGATACCCTGAGTGCTCTCCGAGGCAATAGTGGATCCGTTTTGGAGGGGCCTGGCAGAGTTGTGGCAG
  ATGGCACTGGCACCCGCACTATCATTGTGCCTCCACTGAAAACTCAGCTTGGTGACTGCACTGTAGCAAC
  CCAGGCCTCAGGTCTCCTGAGCAATAAGACTAAGCCAGTCGCTTCAGTGAGTGGGCAGTCATCTGGATGC
  TGTATCACCCCCACAGGGTATCGAGCTCAACGCGGGGGGACCAGTGCAGCACAACCACTCAATCTTAGCC
  AGAACCAGCAGTCATCGGTGGCTCCAACCTCACAGGAGAGAAGCAGCAACCCAGCCCCCCGCAGGCAGCA
  GGCGTTTGTGGCCCCTCTCTCCCAAGCCCCCTACACCTTCCAGCATGGCAGCCCGCTACACTCGACAGGG
  CACCCACACCTTGCCCCGGCCCCTGCTCACCTGCCAAGCCAGGCTCATCTGTATACGTATGCTGCCCCGA
  CTTCTGCTGCTGCACTGGGCTCAACCAGCTCCATTGCTCATCTTTTCTCCCCACAGGGTTCCTCAAGGCA
  TGCTGCAGCCTATACCACTCACCCTAGCACTTTGGTGCACCAGGTCCCTGTCAGTGTTGGGCCCAGCCTC
  CTCACTTCTGCCAGCGTGGCCCCTGCTCAGTACCAACACCAGTTTGCCACCCAATCCTACATTGGGTCTT
  CCCGAGGCTCAACAATTTACACTGGATACCCGCTGAGTCCTACCAAGATCAGCCAGTATTCCTACTTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206176 representing NM_181358
  Red=Cloning site Green=Tags(s)
  
  MVLMFQIRYISQTQGLPAEYLLSAGTKTTRFFNRDPNLGYPLWRLKTPEEHELETGIKSKEARKYIFNCL
  DDMAQVNMSTDLEGTDMLAEKADRREYIDLLKKMLTIDADKRITPLKTLNHQFVTMTHLLDFPHSNHVKS
  CFQNMEICKRRVHMYDTVSQIKSPFTTHVAPNTSTNLTMSFSNQLNTVHNQASVLASSSTAAAATLSLAN
  SDVSLLNYQSALYPSSAAPVPGVAQQGVSLQPGTTQICTQTDPFQQTFIVCPPAFQTGLQATTKHSGFPV
  RMDNAVPIVPQAPAAQPLQIQSGVLTQGSCTPLMVATLHPQVATITPQYAVPFTLSCAAGRPALVEQTAA
  VLQAWPGGTQQILLPSTWQQLPGVALHNSVQPTAMIPEAMGSGQQLADWRNAHSHGNQYSTIMQQPSLLT
  NHVTLATAQPLNVGVAHVVRQQQSSSLPSKKNKQSAPVSSKSSLDVLPSQVYSLVGSSPLRTTSSYNSLV
  PVQDQHQPIIIPDTPSPPVSVITIRSDTDEEEDNKYKPSSSGLKPRSNVISYVTVNDSPDSDSSLSSPYS
  TDTLSALRGNSGSVLEGPGRVVADGTGTRTIIVPPLKTQLGDCTVATQASGLLSNKTKPVASVSGQSSGC
  CITPTGYRAQRGGTSAAQPLNLSQNQQSSVAPTSQERSSNPAPRRQQAFVAPLSQAPYTFQHGSPLHSTG
  HPHLAPAPAHLPSQAHLYTYAAPTSAAALGSTSSIAHLFSPQGSSRHAAAYTTHPSTLVHQVPVSVGPSL
  LTSASVAPAQYQHQFATQSYIGSSRGSTIYTGYPLSPTKISQYSYL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181358
• ORF Size: 2448 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181358.1, NP_852003.1
• RefSeq Size: 6802 bp
• RefSeq ORF: 2451 bp
• Locus ID: 204851
• UniProt ID: Q86Z02
• Cytogenetics: 1p13.2
• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Gene Summary: The protein encoded by this gene belongs to the Ser/Thr family of protein kinases and HIPK subfamily. It phosphorylates homeodomain transcription factors and may also function as a co-repressor for homeodomain transcription factors. Alternative splicing results in four transcript variants encoding four distinct isoforms. [provided by RefSeq, Jul 2008]