UGT2B7 (NM_001074) Human Tagged ORF Clone

CAT#: RG205915

UGT2B7 (tGFP-tagged) - Human UDP glucuronosyltransferase 2 family, polypeptide B7 (UGT2B7)

Product Images

Circular map for RG205915

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: UGT2B7
• Synonyms: UDPGT 2B7; UDPGT2B7; UDPGT 2B9; UDPGTh-2; UDPGTH2; UGT2B9
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205915 representing NM_001074
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGTGAAATGGACTTCAGTAATTTTGCTAATACAACTGAGCTTTTGCTTTAGCTCTGGGAATTGTG
  GAAAGGTGCTGGTGTGGGCAGCAGAATACAGCCATTGGATGAATATAAAGACAATCCTGGATGAGCTTAT
  TCAGAGAGGTCATGAGGTGACTGTACTGGCATCTTCAGCTTCCATTCTTTTTGATCCCAACAACTCATCC
  GCTCTTAAAATTGAAATTTATCCCACATCTTTAACTAAAACTGAGTTGGAGAATTTCATCATGCAACAGA
  TTAAGAGATGGTCAGACCTTCCAAAAGATACATTTTGGTTATATTTTTCACAAGTACAGGAAATCATGTC
  AATATTTGGTGACATAACTAGAAAGTTCTGTAAAGATGTAGTTTCAAATAAGAAATTTATGAAAAAAGTA
  CAAGAGTCAAGATTTGACGTCATTTTTGCAGATGCTATTTTTCCCTGTAGTGAGCTGCTGGCTGAGCTAT
  TTAACATACCCTTTGTGTACAGTCTCAGCTTCTCTCCTGGCTACACTTTTGAAAAGCATAGTGGAGGATT
  TATTTTCCCTCCTTCCTACGTACCTGTTGTTATGTCAGAATTAACTGATCAAATGACTTTCATGGAGAGG
  GTAAAAAATATGATCTATGTGCTTTACTTTGACTTTTGGTTCGAAATATTTGACATGAAGAAGTGGGATC
  AGTTTTATAGTGAAGTTCTAGGAAGACCCACTACATTATCTGAGACAATGGGGAAAGCTGACGTATGGCT
  TATTCGAAACTCCTGGAATTTTCAGTTTCCATATCCACTCTTACCAAATGTTGATTTTGTTGGAGGACTC
  CACTGCAAACCTGCCAAACCCCTGCCTAAGGAAATGGAAGACTTTGTACAGAGCTCTGGAGAAAATGGTG
  TTGTGGTGTTTTCTCTGGGGTCAATGGTCAGTAACATGACAGAAGAAAGGGCCAACGTAATTGCATCAGC
  CCTGGCCCAGATCCCACAAAAGGTTCTGTGGAGATTTGATGGGAATAAACCAGATACCTTAGGTCTCAAT
  ACTCGGCTCTACAAGTGGATACCCCAGAATGACCTTCTAGGTCATCCAAAGACCAGAGCTTTTATAACTC
  ATGGTGGAGCCAATGGCATCTACGAGGCAATCTACCATGGGATCCCTATGGTGGGGATTCCATTGTTTGC
  CGATCAACCTGATAACATTGCTCACATGAAGGCCAGGGGAGCAGCTGTTAGAGTGGACTTCAACACAATG
  TCGAGTACAGACTTGCTGAATGCATTGAAGAGAGTAATTAATGATCCTTCATATAAAGAGAATGTTATGA
  AATTATCAAGAATTCAACATGATCAACCAGTGAAGCCCCTGGATCGAGCAGTCTTCTGGATTGAATTTGT
  CATGCGCCACAAAGGAGCTAAACACCTTCGGGTTGCAGCCCACGACCTCACCTGGTTCCAGTACCACTCT
  TTGGATGTGATTGGGTTCCTGCTGGTCTGTGTGGCAACTGTGATATTTATCGTCACAAAATGTTGTCTGT
  TTTGTTTCTGGAAGTTTGCTAGAAAAGCAAAGAAGGGAAAAAATGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205915 representing NM_001074
  Red=Cloning site Green=Tags(s)
  
  MSVKWTSVILLIQLSFCFSSGNCGKVLVWAAEYSHWMNIKTILDELIQRGHEVTVLASSASILFDPNNSS
  ALKIEIYPTSLTKTELENFIMQQIKRWSDLPKDTFWLYFSQVQEIMSIFGDITRKFCKDVVSNKKFMKKV
  QESRFDVIFADAIFPCSELLAELFNIPFVYSLSFSPGYTFEKHSGGFIFPPSYVPVVMSELTDQMTFMER
  VKNMIYVLYFDFWFEIFDMKKWDQFYSEVLGRPTTLSETMGKADVWLIRNSWNFQFPYPLLPNVDFVGGL
  HCKPAKPLPKEMEDFVQSSGENGVVVFSLGSMVSNMTEERANVIASALAQIPQKVLWRFDGNKPDTLGLN
  TRLYKWIPQNDLLGHPKTRAFITHGGANGIYEAIYHGIPMVGIPLFADQPDNIAHMKARGAAVRVDFNTM
  SSTDLLNALKRVINDPSYKENVMKLSRIQHDQPVKPLDRAVFWIEFVMRHKGAKHLRVAAHDLTWFQYHS
  LDVIGFLLVCVATVIFIVTKCCLFCFWKFARKAKKGKND
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001074
• ORF Size: 1587 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001074.4
• RefSeq Size: 1899 bp
• RefSeq ORF: 1590 bp
• Locus ID: 7364
• UniProt ID: P16662
• Cytogenetics: 4q13.2
• Domains: UDPGT
• Protein Families: Transmembrane
• Protein Pathways: Androgen and estrogen metabolism, Ascorbate and aldarate metabolism, Drug metabolism - cytochrome P450, Drug metabolism - other enzymes, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Pentose and glucuronate interconversions, Porphyrin and chlorophyll metabolism, Retinol metabolism, Starch and sucrose metabolism
• Gene Summary: The protein encoded by this gene belongs to the UDP-glycosyltransferase (UGT) family. UGTs serve a major role in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This protein is localized in the microsome membrane, and has unique specificity for 3,4-catechol estrogens and estriol, suggesting that it may play an important role in regulating the level and activity of these potent estrogen metabolites. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2017]