PTPRN2 (NM_130843) Human Tagged ORF Clone

CAT#: RG205368

PTPRN2 (tGFP-tagged) - Human protein tyrosine phosphatase, receptor type, N polypeptide 2 (PTPRN2), transcript variant 3

Product Images

Circular map for RG205368

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PTPRN2
• Synonyms: IA-2beta; IAR; ICAAR; PTPRP; R-PTP-N2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205368 representing NM_130843
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCCGCCGCTCCCGCTGCTGCTGCTGCTACTGCTGCTGCTGCCGCCACGCGTCCTGCCTGCCGCCC
  CTTCGTCCGTCCCCCGCGGCCGGCAGCTCCCGGGGCGTCTGGGCTGCCTGCTCGAGGAGGGCCTCTGCGG
  AGCGTCCGAGGCCTGTGTGAACGATGGAGTGTTTGGAAGGTGCCAGAAGGTTCCGGCAATGGACTTTTAC
  CGCTACGAGGTGTCGCCCGTGGCCCTGCAGCGCCTGCGCGTGGCGTTGCAGAAGCTTTCCGGCACAGGTT
  TCACGTGGCAGGATGACTATACTCAGTATGTGATGGACCAGGAACTTGCAGACCTCCCGAAAACCTACCT
  GAGGCGTCCTGAAGCATCCAGCCCAGCCAGGCCCTCAAAACACAGCGTTGGCAGCGAGAGGAGGTACAGT
  CGGGAGGGCGGTGCTGCCCTGGCCAACGCCCTCCGACGCCACCTGCCCTTCCTGGAGGCCCTGTCCCAGG
  CCCCAGCCTCAGACGTGCTCGCCAGGACCCATACGGCGCAGGACAGACCCCCCGCTGAGGGTGATGACCG
  CTTCTCCGAGAGCATCCTGACCTATGTGGCCCACACGTCTGCGCTGACCTACCCTCCCGGGCCCCGGACC
  CAGCTCCGCGAGGACCTCCTGCCGCGGACCCTCGGCCAGCTCCAGCCAGATGAGCTCAGCCCTAAGGTGG
  ACAGTGGTGTGGACAGACACCATCTGATGGCGGCCCTCAGTGCCTATGCTGCCCAGAGGCCCCCAGCTCC
  CCCCGGGGAGGGCAGCCTGGAGCCACAGTACCTTCTGCGTGCACCCTCAAGAATGCCCAGGCCTTTGCTG
  GCACCAGCCGCCCCCCAGAAGTGGCCTTCACCTCTGGGAGATTCCGAAGACCCCTCTAGCACAGGCGATG
  GAGCACGGATTCATACCCTCCTGAAGGACCTGCAGAGGCAGCCGGCTGAGGTGAGGGGCCTGAGTGGCCT
  GGAGCTGGACGGCATGGCTGAGCTGATGGCTGGCCTGATGCAAGGCGTGGACCATGGAGTAGCTCGAGGC
  AGCCCTGGGAGAGCGGCCCTGGGAGAGTCTGGAGAACAGGCGGATGGCCCCAAGGCCACCCTCCGTGGAG
  ACAGCTTTCCAGATGACGGAGTGCAGGACGACGATGATAGACTTTACCAAGAGGTCCATCGTCTGAGTGC
  CACACTCGGGGGCCTCCTGCAGGACCACGGGTCTCGACTCTTACCTGGAGCCCTCCCCTTTGCAAGGCCC
  CTCGACATGGAGAGGAAGAAGTCCGAGCACCCTGAGTCTTCCCTGTCTTCAGAAGAGGAGACTGCCGGAG
  TGGAGAACGTCAAGAGCCAGACGTATTCCAAAGATCTGCTGGGGCAGCAGCCGCATTCGGAGCCCGGGGC
  CGCTGCGTTTGGGGAGCTCCAAAACCAGATGCCTGGGCCCTCGAAGGAGGAGCAGAGCCTTCCAGCGGGT
  GCTCAGGAGGCCCTCAGCGACGGCCTGCAATTGGAGGTCCAGCCTTCCGAGGAAGAGGCGCGGGGCTACA
  TCGTGACAGACAGAGAGGTTCTCGGACCAGCAGTGACCTTCAAAGTGAGCGCCAATGTCCAAAACGTGAC
  CACTGAGGATGTGGAGAAGGCCACAGTTGACAACAAAGACAAACTGGAGGAAACCTCTGGACTGAAAATT
  CTTCAAACCGGAGTCGGGTCGAAAAGCAAACTCAAGTTCCTGCCTCCTCAGGCGGAGCAAGAAGACTCCA
  CCAAGTTCATCGCGCTCACCCTGGTCTCCCTCGCCTGCATCCTGGGCGTCCTCCTGGCCTCTGGCCTCAT
  CTACTGCCTCCGCCATAGCTCTCAGCACAGGCTGAAGGAGAAGCTCTCGGGACTAGGGGGCGACCCAGGT
  GCAGATGCCACTGCCGCCTACCAGGAGCTGTGCCGCCAGCGTATGGCCACGCGGCCACCAGACCGACCTG
  AGGGCCCGCACACGTCACGCATCAGCAGCGTCTCATCCCAGTTCAGCGACGGGCCGATCCCCAGCCCCTC
  CGCACGCAGCAGCGCCTCATCCTGGTCCGAGGAGCCTGTGCAGTCCAACATGGACATCTCAACCGGCCAC
  ATGATCCTGTCCTACATGGAGGACCACCTGAAGAACAAGAACCGGCTGGAGAAGGAGTGGGAAGCGCTGT
  GCGCCTACCAGGCGGAGCCCAACAGCTCGTTCGTGGCCCAGAGGGAGGAGAACGTGCCCAAGAACCGCTC
  CCTGGCTGTGCTGACCTATGACCACTCCCGGGTCCTGCTGAAGGCGGAGAACAGCCACAGCCACTCAGAC
  TACATCAACGCTAGCCCTATCATGGATCACGACCCGAGGAACCCCGCGTACATCGCCACCCAGGGACCGC
  TGCCCGCCACCGTGGCTGACTTTTGGCAGATGGTGTGGGAGAGCGGCTGCGTGGTGATCGTCATGCTGAC
  ACCCCTCGCGGAGAACGGCGTCCGGCAGTGCTACCACTACTGGCCGGATGAAGGCTCCAATCTCTACCAC
  ATCTATGAGGTGAACCTGGTCTCCGAGCACATCTGGTGTGAGGACTTCCTGGTGAGGAGCTTCTATCTGA
  AGAACCTGCAGACCAACGAGACGCGCACCGTGACGCAGTTCCACTTCCTGAGTTGGTATGACCGAGGAGT
  CCCTTCCTCCTCAAGGTCCCTCCTGGACTTCCGCAGAAAAGTAAACAAGTGCTACAGGGGCCGTTCTTGT
  CCAATAATTGTTCATTGCAGTGACGGTGCAGGCCGGAGCGGCACCTACGTCCTGATCGACATGGTTCTCA
  ACAAGATGGCCAAAGGTGCTAAAGAGATTGATATCGCAGCGACCCTGGAGCACTTGAGGGACCAGAGACC
  CGGCATGGTCCAGACGAAGGAGCAGTTTGAGTTCGCGCTGACAGCCGTGGCTGAGGAGGTGAACGCCATC
  CTCAAGGCCCTTCCCCAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205368 representing NM_130843
  Red=Cloning site Green=Tags(s)
  
  MGPPLPLLLLLLLLLPPRVLPAAPSSVPRGRQLPGRLGCLLEEGLCGASEACVNDGVFGRCQKVPAMDFY
  RYEVSPVALQRLRVALQKLSGTGFTWQDDYTQYVMDQELADLPKTYLRRPEASSPARPSKHSVGSERRYS
  REGGAALANALRRHLPFLEALSQAPASDVLARTHTAQDRPPAEGDDRFSESILTYVAHTSALTYPPGPRT
  QLREDLLPRTLGQLQPDELSPKVDSGVDRHHLMAALSAYAAQRPPAPPGEGSLEPQYLLRAPSRMPRPLL
  APAAPQKWPSPLGDSEDPSSTGDGARIHTLLKDLQRQPAEVRGLSGLELDGMAELMAGLMQGVDHGVARG
  SPGRAALGESGEQADGPKATLRGDSFPDDGVQDDDDRLYQEVHRLSATLGGLLQDHGSRLLPGALPFARP
  LDMERKKSEHPESSLSSEEETAGVENVKSQTYSKDLLGQQPHSEPGAAAFGELQNQMPGPSKEEQSLPAG
  AQEALSDGLQLEVQPSEEEARGYIVTDREVLGPAVTFKVSANVQNVTTEDVEKATVDNKDKLEETSGLKI
  LQTGVGSKSKLKFLPPQAEQEDSTKFIALTLVSLACILGVLLASGLIYCLRHSSQHRLKEKLSGLGGDPG
  ADATAAYQELCRQRMATRPPDRPEGPHTSRISSVSSQFSDGPIPSPSARSSASSWSEEPVQSNMDISTGH
  MILSYMEDHLKNKNRLEKEWEALCAYQAEPNSSFVAQREENVPKNRSLAVLTYDHSRVLLKAENSHSHSD
  YINASPIMDHDPRNPAYIATQGPLPATVADFWQMVWESGCVVIVMLTPLAENGVRQCYHYWPDEGSNLYH
  IYEVNLVSEHIWCEDFLVRSFYLKNLQTNETRTVTQFHFLSWYDRGVPSSSRSLLDFRRKVNKCYRGRSC
  PIIVHCSDGAGRSGTYVLIDMVLNKMAKGAKEIDIAATLEHLRDQRPGMVQTKEQFEFALTAVAEEVNAI
  LKALPQ
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130843
• ORF Size: 2958 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130843.1, NP_570858.1
• RefSeq Size: 4680 bp
• RefSeq ORF: 2961 bp
• Locus ID: 5799
• UniProt ID: Q92932
• Cytogenetics: 7q36.3
• Domains: Y_phosphatase, PTPc_motif
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Type I diabetes mellitus
• Gene Summary: This gene encodes a protein with sequence similarity to receptor-like protein tyrosine phosphatases. However, tyrosine phosphatase activity has not been experimentally validated for this protein. Studies of the rat ortholog suggest that the encoded protein may instead function as a phosphatidylinositol phosphatase with the ability to dephosphorylate phosphatidylinositol 3-phosphate and phosphatidylinositol 4,5-diphosphate, and this function may be involved in the regulation of insulin secretion. This protein has been identified as an autoantigen in insulin-dependent diabetes mellitus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]