DIAPH3 (NM_030932) Human Tagged ORF Clone

CAT#: RG205246

DIAPH3 (tGFP-tagged) - Human diaphanous homolog 3 (Drosophila) (DIAPH3), transcript variant 2

Product Images

Circular map for RG205246

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DIAPH3
• Synonyms: AN; AUNA1; DIA2; diap3; DRF3; mDia2; NSDAN
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205246 representing NM_030932
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGAGGAGAGGAGCCTTTCCTTATTGGTCAAAGCCGTGGATCCCAGACACCCCAATATGATGACAG
  ATGTGGTTAAACTTCTCTCTGCGGTATGCATTGTAGGGGAAGAAAGCATCCTTGAAGAAGTTTTAGAAGC
  TTTAACTTCAGCTGGTGAAGAAAAAAAAATTGACAGATTTTTTTGTATTGTGGAAGGCCTCCGGCACAAT
  TCAGTTCAACTGCAAGTAGCTTGTATGCAGCTCATCAATGCCCTGGTTACATCTCCTGATGATTTGGATT
  TCAGGCTTCACATCAGAAATGAATTTATGCGTTGTGGATTGAAAGAGATATTGCCAAATTTAAAATGCAT
  TAAGAATGATGGCCTGGATATCCAACTTAAAGTCTTTGATGAGCATAAAGAAGAAGATTTGTTTGAGTTA
  TCCCATCGCCTTGAAGATATTAGAGCTGAACTTGATGAAGCATATGATGTTTACAACATGGTGTGGAGCA
  CAGTTAAAGAAACTAGAGCAGAGGGATATTTTATTTCTATTCTTCAGCATCTGTTGCTGATTCGAAATGA
  TTATTTTATAAGGCAACAATACTTCAAATTAATTGATGAGTGTGTATCCCAGATTGTATTGCATAGAGAT
  GGAATGGATCCAGACTTCACATATCGAAAAAGACTAGATTTAGATTTAACCCAGTTTGTAGACATTTGCA
  TAGATCAAGCAAAACTAGAAGAGTTTGAAGAGAAAGCATCAGAACTTTACAAGAAATTTGAAAAAGAGTT
  TACCGACCACCAAGAAACTCAGGCTGAATTGCAGAAAAAAGAGGCAAAGATTAATGAGCTTCAAGCAGAG
  CTACAAGCTTTTAAGTCTCAGTTTGGTGCCTTGCCAGCTGATTGTAATATTCCTTTGCCTCCCTCTAAAG
  AAGGTGGAACTGGCCACTCAGCACTTCCTCCTCCGCCTCCACTGCCTTCTGGTGGAGGGGTGCCGCCTCC
  ACCTCCTCCCCCACCACCTCCTCCACTTCCAGGAATGCGGATGCCATTCAGTGGTCCTGTGCCTCCACCA
  CCTCCCCTGGGATTCCTTGGAGGACAAAATTCTCCTCCTCTACCAATCCTGCCATTTGGGTTGAAACCAA
  AGAAAGAATTTAAACCTGAAATCAGCATGAGAAGATTGAATTGGTTAAAGATCAGACCTCATGAAATGAC
  TGAAAACTGTTTCTGGATAAAAGTAAATGAAAATAAGTATGAAAACGTGGATTTGCTTTGTAAACTTGAG
  AATACATTTTGTTGCCAACAAAAAGAGAGAAGAGAAGAGGAAGATATTGAAGAGAAGAAATCGATTAAGA
  AAAAAATTAAAGAACTTAAGTTTTTAGATTCTAAAATTGCCCAGAACCTTTCAATCTTCCTGAGCTCTTT
  TCGGGTGCCATATGAGGAAATCAGAATGATGATATTGGAAGTAGATGAAACACGGTTGGCAGAGTCTATG
  ATTCAGAACTTAATAAAGCATCTTCCTGATCAAGAGCAATTAAATTCATTGTCTCAGTTCAAGAGTGAAT
  ATAGCAACTTATGTGAACCTGAGCAGTTTGTGGTTGTGATGAGCAATGTGAAGAGACTACGGCCACGGCT
  CAGTGCTATTCTCTTTAAGCTTCAGTTTGAAGAGCAGGTGAACAACATCAAACCTGACATCATGGCTGTC
  AGTACTGCCTGCGAAGAGATAAAGAAGAGCAAAAGCTTTAGCAAGTTGCTGGAACTTGTATTGCTAATGG
  GAAACTACATGAATGCTGGCTCCCGGAATGCTCAAACCTTCGGATTTAACCTTAGCTCTCTCTGTAAACT
  AAAGGACACAAAATCAGCAGATCAGAAAACAACGCTACTTCATTTCCTGGTAGAAATATGTGAAGAGAAG
  TACCCTGATATACTGAATTTTGTGGATGATTTGGAACCTTTAGACAAAGCTAGTAAAGTCTCTGTAGAAA
  CGCTGGAAAAGAATTTGAGGCAGATGGGAAGGCAGCTTCAACAGCTTGAGAAGGAATTGGAAACCTTTCC
  CCCTCCTGAGGACTTGCATGACAAGTTTGTGACAAAGATGTCCAGATTTGTTATCAGTGCAAAAGAACAA
  TATGAGACACTTTCGAAGTTACACGAAAACATGGAAAAGTTATACCAGAGTATAATAGGATACTATGCCA
  TTGATGTGAAGAAGGTGTCTGTGGAAGACTTTCTTACTGACCTGAATAACTTCAGAACCACATTCATGCA
  AGCAATAAAGGAGAATATCAAAAAAAGAGAAGCAGAGGAAAAAGAAAAACGTGTCAGAATAGCTAAAGAA
  TTAGCAGAGCGAGAAAGACTCGAACGCCAACAAAAGAAAAAGCGTTTATTAGAAATGAAGACTGAGGGTG
  ATGAGACAGGAGTGATGGATAATCTGCTGGAGGCCTTGCAGTCCGGGGCTGCCTTCCGCGACAGAAGAAA
  AAGGACACCGATGCCAAAAGATGTTCGGCAGAGTCTCAGTCCAATGTCTCAGAGGCCTGTTCTGAAAGTT
  TGTAACCATGGTAATAAACCGTATTTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205246 representing NM_030932
  Red=Cloning site Green=Tags(s)
  
  MSEERSLSLLVKAVDPRHPNMMTDVVKLLSAVCIVGEESILEEVLEALTSAGEEKKIDRFFCIVEGLRHN
  SVQLQVACMQLINALVTSPDDLDFRLHIRNEFMRCGLKEILPNLKCIKNDGLDIQLKVFDEHKEEDLFEL
  SHRLEDIRAELDEAYDVYNMVWSTVKETRAEGYFISILQHLLLIRNDYFIRQQYFKLIDECVSQIVLHRD
  GMDPDFTYRKRLDLDLTQFVDICIDQAKLEEFEEKASELYKKFEKEFTDHQETQAELQKKEAKINELQAE
  LQAFKSQFGALPADCNIPLPPSKEGGTGHSALPPPPPLPSGGGVPPPPPPPPPPPLPGMRMPFSGPVPPP
  PPLGFLGGQNSPPLPILPFGLKPKKEFKPEISMRRLNWLKIRPHEMTENCFWIKVNENKYENVDLLCKLE
  NTFCCQQKERREEEDIEEKKSIKKKIKELKFLDSKIAQNLSIFLSSFRVPYEEIRMMILEVDETRLAESM
  IQNLIKHLPDQEQLNSLSQFKSEYSNLCEPEQFVVVMSNVKRLRPRLSAILFKLQFEEQVNNIKPDIMAV
  STACEEIKKSKSFSKLLELVLLMGNYMNAGSRNAQTFGFNLSSLCKLKDTKSADQKTTLLHFLVEICEEK
  YPDILNFVDDLEPLDKASKVSVETLEKNLRQMGRQLQQLEKELETFPPPEDLHDKFVTKMSRFVISAKEQ
  YETLSKLHENMEKLYQSIIGYYAIDVKKVSVEDFLTDLNNFRTTFMQAIKENIKKREAEEKEKRVRIAKE
  LAERERLERQQKKKRLLEMKTEGDETGVMDNLLEALQSGAAFRDRRKRTPMPKDVRQSLSPMSQRPVLKV
  CNHGNKPYL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030932
• ORF Size: 2547 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030932.3, NP_112194.2
• RefSeq Size: 2745 bp
• RefSeq ORF: 2550 bp
• Locus ID: 81624
• UniProt ID: Q9NSV4
• Cytogenetics: 13q21.2
• Protein Pathways: Regulation of actin cytoskeleton
• Gene Summary: This gene encodes a member of the diaphanous subfamily of the formin family. Members of this family are involved in actin remodeling and regulate cell movement and adhesion. Mutations in this gene are associated with autosomal dominant auditory neuropathy 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]