LARS2 (NM_015340) Human Tagged ORF Clone

CAT#: RG205207

LARS2 (tGFP-tagged) - Human leucyl-tRNA synthetase 2, mitochondrial (LARS2), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RG205207

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LARS2
• Synonyms: HLASA; LEURS; mtLeuRS; PRLTS4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205207 representing NM_015340
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCTGTTTGGCAGAGATTGGGTTTTTATGCCTCTCTTCTGAAAAGACAGCTAAATGGTGGGCCAG
  ATGTCATCAAGTGGGAAAGGAGAGTAATTCCCGGATGTACCAGAAGCATCTACAGTGCCACGGGAAAGTG
  GACAAAAGAGTATACATTGCAGACAAGAAAGGATGTTGAGAAATGGTGGCATCAACGAATAAAAGAACAG
  GCCTCCAAAATTTCAGAAGCTGATAAATCGAAGCCAAAATTTTACGTGCTTTCCATGTTCCCTTATCCTT
  CTGGTAAGCTGCACATGGGCCATGTGCGTGTCTACACCATCAGCGACACCATAGCACGGTTCCAGAAGAT
  GAGAGGGATGCAGGTCATCAACCCCATGGGATGGGATGCTTTTGGATTGCCTGCTGAAAATGCCGCAGTC
  GAGAGGAATCTACATCCACAAAGTTGGACACAAAGTAATATTAAACACATGAGGAAACAGCTTGATCGTC
  TGGGCCTGTGTTTCAGCTGGGATAGGGAAATAACTACGTGTTTGCCAGATTACTACAAGTGGACTCAGTA
  TCTCTTTATTAAACTGTATGAGGCTGGGCTGGCCTATCAAAAGGAGGCCCTGGTTAACTGGGACCCAGTG
  GATCAAACAGTGCTTGCCAATGAGCAGGTGGATGAACATGGCTGTTCATGGCGTTCTGGAGCAAAGGTGG
  AACAGAAGTACCTCAGACAATGGTTTATTAAGACAACCGCTTATGCAAAGGCCATGCAGGACGCGTTGGC
  AGACCTTCCAGAATGGTATGGAATAAAAGGCATGCAAGCCCACTGGATTGGGGACTGTGTGGGCTGCCAC
  CTGGACTTCACATTAAAGGTTCATGGGCAAGCCACGGGCGAAAAGCTGACTGCCTATACGGCCACCCCTG
  AAGCCATTTATGGCACCTCCCACGTGGCCATCTCGCCCAGCCACAGACTCCTACATGGGCACAGCTCTCT
  GAAGGAAGCCTTGAGGATGGCCCTTGTCCCTGGCAAAGATTGCCTCACGCCTGTAATGGCTGTGAACATG
  CTCACCCAGCAGGAGGTCCCTGTCGTTATTTTGGCCAAAGCTGACTTGGAAGGCTCTCTGGATTCAAAAA
  TAGGAATTCCCAGTACTAGCTCAGAGGACACCATCTTAGCCCAAACCCTGGGCCTGGCCTACTCTGAAGT
  CATTGAAACTTTGCCAGATGGCACAGAGAGACTGAGCAGCTCTGCTGAGTTCACAGGTATGACCCGGCAG
  GATGCTTTTCTAGCCCTGACTCAGAAAGCCCGGGGGAAGAGAGTGGGTGGAGACGTGACAAGTGATAAAC
  TGAAAGACTGGCTGATTTCACGGCAGCGGTACTGGGGCACACCAATCCCCATTGTCCACTGCCCAGTCTG
  TGGCCCCACACCTGTGCCCCTGGAGGACTTGCCTGTGACCCTGCCCAACATCGCATCTTTCACTGGCAAG
  GGAGGCCCCCCACTGGCCATGGCTTCAGAGTGGGTGAACTGCTCCTGCCCAAGGTGCAAGGGAGCAGCCA
  AGAGAGAGACAGACACGATGGATACCTTTGTTGATTCTGCTTGGTACTACTTCAGATACACTGACCCTCA
  TAATCCACACAGCCCTTTTAACACAGCAGTGGCCGATTACTGGATGCCTGTGGATTTGTACATTGGAGGG
  AAAGAACATGCCGTCATGCACTTGTTCTATGCAAGATTCTTTAGTCATTTTTGCCATGATCAAAAAATGG
  TTAAACATAGGGAGCCTTTTCATAAGCTGCTGGCCCAAGGCCTTATCAAGGGGCAGACATTCCGCCTACC
  ATCTGGACAGTATCTACAGAGAGAGGAAGTGGATCTCACAGGTTCCGTTCCTGTTCATGCAAAAACGAAA
  GAGAAGTTAGAGGTGACGTGGGAGAAGATGAGTAAGTCCAAACACAACGGGGTGGACCCAGAGGAAGTTG
  TGGAGCAGTATGGGATCGACACGATTCGGCTCTACATCCTTTTTGCTGCCCCTCCTGAGAAGGATATCTT
  GTGGGATGTGAAAACTGATGCTCTCCCTGGGGTGCTGAGATGGCAACAACGACTGTGGACCTTGACAACT
  CGGTTTATTGAGGCCAGGGCTTCTGGGAAGTCTCCCCAGCCTCAGCTGCTGAGTAACAAGGAGAAAGCCG
  AGGCCAGGAAGCTCTGGGAGTACAAGAACTCCGTCATCTCTCAGGTGACCACCCATTTCACAGAGGACTT
  CTCACTGAATTCTGCAATTTCTCAGCTGATGGGACTCAGCAATGCCCTCTCGCAAGCCTCTCAGAGCGTC
  ATTCTCCACAGCCCCGAGTTTGAGGATGCTTTGTGTGCCCTGATGGTGATGGCTGCTCCACTGGCCCCTC
  ATGTAACCTCAGAGATCTGGGCAGGCCTGGCGCTGGTGCCGAGGAAGCTCTGTGCCCACTACACTTGGGA
  TGCCAGTGTGCTGCTCCAGGCATGGCCTGCTGTGGACCCGGAGTTCCTGCAGCAGCCTGAGGTTGTCCAG
  ATGGCAGTTCTGATCAACAATAAAGCTTGTGGCAAAATTCCTGTGCCCCAACAAGTTGCCCGGGACCAGG
  ACAAAGTCCACGAATTTGTTCTTCAAAGCGAGCTGGGTGTCAGGCTTTTGCAAGGACGAAGCATCAAGAA
  GTCCTTCCTTTCCCCGAGAACTGCCCTCATCAACTTCCTGGTGCAAGAT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205207 representing NM_015340
  Red=Cloning site Green=Tags(s)
  
  MASVWQRLGFYASLLKRQLNGGPDVIKWERRVIPGCTRSIYSATGKWTKEYTLQTRKDVEKWWHQRIKEQ
  ASKISEADKSKPKFYVLSMFPYPSGKLHMGHVRVYTISDTIARFQKMRGMQVINPMGWDAFGLPAENAAV
  ERNLHPQSWTQSNIKHMRKQLDRLGLCFSWDREITTCLPDYYKWTQYLFIKLYEAGLAYQKEALVNWDPV
  DQTVLANEQVDEHGCSWRSGAKVEQKYLRQWFIKTTAYAKAMQDALADLPEWYGIKGMQAHWIGDCVGCH
  LDFTLKVHGQATGEKLTAYTATPEAIYGTSHVAISPSHRLLHGHSSLKEALRMALVPGKDCLTPVMAVNM
  LTQQEVPVVILAKADLEGSLDSKIGIPSTSSEDTILAQTLGLAYSEVIETLPDGTERLSSSAEFTGMTRQ
  DAFLALTQKARGKRVGGDVTSDKLKDWLISRQRYWGTPIPIVHCPVCGPTPVPLEDLPVTLPNIASFTGK
  GGPPLAMASEWVNCSCPRCKGAAKRETDTMDTFVDSAWYYFRYTDPHNPHSPFNTAVADYWMPVDLYIGG
  KEHAVMHLFYARFFSHFCHDQKMVKHREPFHKLLAQGLIKGQTFRLPSGQYLQREEVDLTGSVPVHAKTK
  EKLEVTWEKMSKSKHNGVDPEEVVEQYGIDTIRLYILFAAPPEKDILWDVKTDALPGVLRWQQRLWTLTT
  RFIEARASGKSPQPQLLSNKEKAEARKLWEYKNSVISQVTTHFTEDFSLNSAISQLMGLSNALSQASQSV
  ILHSPEFEDALCALMVMAAPLAPHVTSEIWAGLALVPRKLCAHYTWDASVLLQAWPAVDPEFLQQPEVVQ
  MAVLINNKACGKIPVPQQVARDQDKVHEFVLQSELGVRLLQGRSIKKSFLSPRTALINFLVQD
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015340
• ORF Size: 2709 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015340.3, NP_056155.1
• RefSeq Size: 4203 bp
• RefSeq ORF: 2712 bp
• Locus ID: 23395
• UniProt ID: Q15031
• Cytogenetics: 3p21.31
• Domains: tRNA-synt_1
• Protein Families: Druggable Genome
• Protein Pathways: Aminoacyl-tRNA biosynthesis, Valine, leucine and isoleucine biosynthesis
• Gene Summary: This gene encodes a class 1 aminoacyl-tRNA synthetase, mitochondrial leucyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. [provided by RefSeq, Jul 2008]