MCEE (NM_032601) Human Tagged ORF Clone
CAT#: RG205018
- TrueORF®
MCEE (tGFP-tagged) - Human methylmalonyl CoA epimerase (MCEE)
ORF Plasmid: DDK
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
AAV Particle: DDK
"NM_032601" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | MCEE |
Synonyms | GLOD2; MCE |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG205018 representing NM_032601
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCGGGTGCTGAAGGCTGCAGCCGCGAATGCCGTAGGGCTTTTTTCCAGACTTCAAGCTCCCATTC CAACAGTAAGAGCTTCTTCCACATCACAGCCCTTGGATCAAGTGACAGGTTCTGTGTGGAACCTGGGTCG ACTCAACCATGTAGCCATAGCAGTGCCAGATTTGGAAAAGGCTGCAGCATTTTATAAGAATATTCTGGGG GCCCAGGTAAGTGAAGCGGTCCCTCTTCCTGAACATGGAGTATCTGTTGTTTTTGTCAACCTGGGAAATA CCAAGATGGAACTGCTTCATCCATTGGGACTTGACAGTCCAATTGCAGGTTTTCTGCAGAAAAACAAGGC TGGAGGAATGCATCACATCTGCATCGAGGTGGATAATATTAATGCAGCTGTGATGGATTTGAAAAAAAAG AAGATCCGCAGTCTAAGTGAAGAGGTCAAAATAGGAGCACATGGAAAACCAGTGATTTTTCTCCATCCTA AAGACTGTGGTGGAGTCCTTGTGGAACTGGAGCAAGCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG205018 representing NM_032601
Red=Cloning site Green=Tags(s) MARVLKAAAANAVGLFSRLQAPIPTVRASSTSQPLDQVTGSVWNLGRLNHVAIAVPDLEKAAAFYKNILG AQVSEAVPLPEHGVSVVFVNLGNTKMELLHPLGLDSPIAGFLQKNKAGGMHHICIEVDNINAAVMDLKKK KIRSLSEEVKIGAHGKPVIFLHPKDCGGVLVELEQA TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_032601 |
ORF Size | 528 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_032601.2, NP_115990.2 |
RefSeq Size | 850 bp |
RefSeq ORF | 531 bp |
Locus ID | 84693 |
UniProt ID | Q96PE7 |
Cytogenetics | 2p13.3 |
Protein Pathways | Metabolic pathways, Propanoate metabolism, Valine, leucine and isoleucine degradation |
Gene Summary | The product of this gene catalyzes the interconversion of D- and L-methylmalonyl-CoA during the degradation of branched chain amino acids. odd chain-length fatty acids, and other metabolites. Mutations in this gene result in methylmalonyl-CoA epimerase deficiency, which is presented as mild to moderate methylmalonic aciduria. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC205018 | MCEE (Myc-DDK-tagged)-Human methylmalonyl CoA epimerase (MCEE) |
USD 300.00 |
|
RC205018L1 | Lenti ORF clone of Human methylmalonyl CoA epimerase (MCEE), Myc-DDK-tagged |
USD 600.00 |
|
RC205018L2 | Lenti ORF clone of Human methylmalonyl CoA epimerase (MCEE), mGFP tagged |
USD 600.00 |
|
RC205018L3 | Lenti ORF clone of Human methylmalonyl CoA epimerase (MCEE), Myc-DDK-tagged |
USD 600.00 |
|
RC205018L4 | Lenti ORF clone of Human methylmalonyl CoA epimerase (MCEE), mGFP tagged |
USD 600.00 |
|
SC125797 | MCEE (untagged)-Human methylmalonyl CoA epimerase (MCEE) |
USD 300.00 |
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