CREB3L1 (NM_052854) Human Tagged ORF Clone

CAT#: RG204968

CREB3L1 (tGFP-tagged) - Human cAMP responsive element binding protein 3-like 1 (CREB3L1)

Product Images

Circular map for RG204968

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CREB3L1
• Synonyms: OASIS; OI16
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204968 representing NM_052854
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGCCGTCTTGGAACCCTTCCCGGCCGACAGGCTGTTCCCCGGATCCAGCTTCCTGGACTTGGGGG
  ATCTGAACGAGTCGGACTTCCTCAACAATGCGCACTTTCCTGAGCACCTGGACCACTTTACGGAGAACAT
  GGAGGACTTCTCCAATGACCTGTTCAGCAGCTTCTTTGATGACCCTGTGCTGGATGAGAAGAGCCCTCTA
  TTGGACATGGAACTGGACTCCCCTACGCCAGGCATCCAGGCGGAGCACAGCTACTCCCTGAGCGGCGACT
  CAGCGCCCCAGAGCCCCCTTGTGCCCATCAAGATGGAGGACACCACCCAAGATGCAGAGCATGGAGCATG
  GGCGCTGGGACACAAACTGTGCTCCATCATGGTGAAGCAGGAGCAGAGCCCGGAGCTGCCCGTGGACCCT
  CTGGCTGCCCCCTCGGCCATGGCTGCCGCGGCCGCCATGGCCACCACCCCGCTGCTGGGCCTCAGCCCCT
  TGTCCAGGCTGCCCATCCCCCACCAGGCCCCGGGAGAGATGACTCAGCTGCCAGTGATCAAAGCAGAGCC
  TCTGGAGGTGAACCAGTTCCTCAAAGTGACACCGGAGGACCTGGTGCAGATGCCTCCGACGCCCCCCAGC
  AGCCATGGCAGTGACAGCGACGGCTCCCAGAGTCCCCGCTCTCTGCCCCCCTCCAGCCCTGTCAGGCCCA
  TGGCGCGCTCCTCCACGGCCATCTCCACCTCCCCACTCCTCACTGCCCCTCACAAATTACAGGGGACATC
  AGGGCCACTGCTCCTGACAGAGGAGGAGAAGCGGACCCTGATTGCTGAGGGCTACCCCATCCCCACAAAA
  CTCCCCCTCACCAAAGCCGAGGAGAAGGCCTTGAAGAGAGTCCGGAGGAAAATCAAGAACAAGATCTCAG
  CCCAGGAGAGCCGTCGTAAGAAGAAGGAGTATGTGGAGTGTCTAGAAAAGAAGGTGGAGACATTTACATC
  TGAGAACAATGAACTGTGGAAGAAGGTGGAGACCCTGGAGAATGCCAACAGGACCCTGCTCCAGCAGCTG
  CAGAAACTCCAGACTCTGGTCACCAACAAGATCTCCAGACCTTACAAGATGGCCGCCACCCAGACTGGGA
  CCTGCCTCATGGTGGCAGCCTTGTGCTTTGTTCTGGTGCTGGGCTCCCTCGTGCCCTGCCTTCCCGAGTT
  CTCCTCCGGCTCCCAGACTGTGAAGGAAGACCCCCTGGCCGCAGACGGCGTCTACACGGCCAGCCAGATG
  CCCTCCCGAAGCCTCCTATTCTACGATGACGGGGCAGGCTTATGGGAAGATGGCCGCAGCACCCTGCTGC
  CCATGGAGCCCCCAGATGGCTGGGAAATCAACCCCGGGGGGCCGGCAGAGCAGCGGCCCCGGGACCACCT
  GCAGCATGATCACCTGGACAGCACCCACGAGACCACCAAGTACCTGAGTGAGGCCTGGCCTAAAGACGGT
  GGAAACGGCACCAGCCCCGACTTCTCCCACTCCAAGGAGTGGTTCCACGACAGGGATCTGGGCCCCAACA
  CCACCATCAAACTCTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204968 representing NM_052854
  Red=Cloning site Green=Tags(s)
  
  MDAVLEPFPADRLFPGSSFLDLGDLNESDFLNNAHFPEHLDHFTENMEDFSNDLFSSFFDDPVLDEKSPL
  LDMELDSPTPGIQAEHSYSLSGDSAPQSPLVPIKMEDTTQDAEHGAWALGHKLCSIMVKQEQSPELPVDP
  LAAPSAMAAAAAMATTPLLGLSPLSRLPIPHQAPGEMTQLPVIKAEPLEVNQFLKVTPEDLVQMPPTPPS
  SHGSDSDGSQSPRSLPPSSPVRPMARSSTAISTSPLLTAPHKLQGTSGPLLLTEEEKRTLIAEGYPIPTK
  LPLTKAEEKALKRVRRKIKNKISAQESRRKKKEYVECLEKKVETFTSENNELWKKVETLENANRTLLQQL
  QKLQTLVTNKISRPYKMAATQTGTCLMVAALCFVLVLGSLVPCLPEFSSGSQTVKEDPLAADGVYTASQM
  PSRSLLFYDDGAGLWEDGRSTLLPMEPPDGWEINPGGPAEQRPRDHLQHDHLDSTHETTKYLSEAWPKDG
  GNGTSPDFSHSKEWFHDRDLGPNTTIKLS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_052854
• ORF Size: 1557 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_052854.4
• RefSeq Size: 2702 bp
• RefSeq ORF: 1560 bp
• Locus ID: 90993
• UniProt ID: Q96BA8
• Cytogenetics: 11p11.2
• Domains: BRLZ
• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease, Melanogenesis, Prostate cancer
• Gene Summary: The protein encoded by this gene is normally found in the membrane of the endoplasmic reticulum (ER). However, upon stress to the ER, the encoded protein is cleaved and the released cytoplasmic transcription factor domain translocates to the nucleus. There it activates the transcription of target genes by binding to box-B elements. [provided by RefSeq, Jun 2013]