ALDH4A1 (NM_170726) Human Tagged ORF Clone

CAT#: RG204884

ALDH4A1 (tGFP-tagged) - Human aldehyde dehydrogenase 4 family, member A1 (ALDH4A1), nuclear gene encoding mitochondrial protein, transcript variant P5CDhS

Product Images

Circular map for RG204884

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ALDH4A1
• Synonyms: ALDH4; P5CD; P5CDh
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204884 representing NM_170726
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTGCCGGCGCCCGCGCTCCGCCGCGCCCTGCTGTCCCGCCCCTGGACCGGGGCCGGCCTGCGGT
  GGAAGCACACCTCCTCCCTGAAGGTGGCCAACGAGCCCGTCTTAGCCTTCACGCAGGGCAGCCCTGAGCG
  AGATGCCCTGCAAAAGGCCTTGAAGGACCTGAAGGGCCGGATGGAAGCCATCCCATGCGTGGTGGGGGAT
  GAGGAGGTGTGGACGTCGGACGTGCAGTACCAAGTGTCGCCTTTTAACCATGGACATAAGGTGGCCAAGT
  TCTGTTATGCAGACAAGAGCCTGCTCAACAAAGCCATTGAGGCTGCCCTGGCTGCCCGGAAAGAGTGGGA
  CCTGAAGCCTATTGCAGACCGGGCCCAGATCTTCCTGAAGGCGGCAGACATGCTGAGTGGGCCGCGCAGG
  GCTGAGATCCTCGCCAAGACCATGGTGGGACAGGGTAAGACCGTGATCCAAGCGGAGATTGACGCTGCAG
  CGGAACTCATCGACTTCTTCCGGTTCAATGCCAAGTATGCGGTGGAGCTGGAGGGGCAGCAGCCCATCAG
  CGTGCCCCCGAGCACCAACAGCACGGTGTACCGGGGTCTGGAGGGCTTCGTGGCGGCCATCTCGCCCTTT
  AACTTCACTGCAATCGGCGGCAACCTGGCGGGGGCACCGGCCCTGATGGGCAACGTGGTCCTATGGAAGC
  CCAGTGACACTGCCATGCTGGCCAGCTATGCTGTCTACCGCATCCTTCGGGAGGCTGGCCTGCCCCCCAA
  CATCATCCAGTTTGTGCCAGCTGATGGGCCCCTATTTGGGGACACTGTCACCAGCTCAGAGCACCTCTGT
  GGCATCAACTTCACAGGCAGTGTGCCCACCTTCAAACACCTGTGGAAGCAGGTGGCCCAGAACCTGGACC
  GGTTCCACACCTTCCCACGCCTGGCTGGAGAGTGCGGCGGAAAGAACTTCCACTTCGTGCACCGCTCGGC
  CGACGTGGAGAGCGTGGTGAGCGGGACCCTCCGCTCAGCCTTCGAGTACGGTGGCCAGAAGTGTTCCGCG
  TGCTCGCGTCTCTACGTGCCGCACTCGCTGTGGCCGCAGATCAAAGGGCGGCTGCTGGAGGAGCACAGTC
  GGATCAAAGTGGGCGACCCTGCAGAGGATTTTGGGACCTTCTTCTCTGCAGTGATTGATGCCAAGTCCTT
  TGCCCGTATCAAGAAGTGGCTGGAGCACGCACGCTCCTCACCCAGCCTCACCATCCTGGCCGGGGGCAAG
  TGTGATGACTCCGTGGGCTACTTTGTGGAGCCCTGCATCGTGGAGAGCAAGGACCCTCAGGAGCCCATCA
  TGAAGGAGGAGATCTTCGGGCCTGTACTGTCTGTGTACGTCTACCCGGATGACAAGTACAAGGAGACGCT
  GCAGCTGGTTGACAGCACCACCAGCTATGGCCTCACGGGGGCAGTGTTCTCCCAGGATAAGGACGTCGTG
  CAGGAGGCCACAAAGGTGCTGAGGAATGCTGCCGGCAACTTCTACATCAACGACAAGTCCACTGGCTCGA
  TAGTGGGCCAGCAGCCCTTTGGGGGGGCCCGAGCCTCTGGAACCAATGACAAGCCAGGGGGCCCACACTA
  CATCCTGCGCTGGACGTCGCCGCAGGTCATCAAGGAGACACATAAGCCCCTGGGGGACTGGAGCTACGCG
  TACATGCAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204884 representing NM_170726
  Red=Cloning site Green=Tags(s)
  
  MLLPAPALRRALLSRPWTGAGLRWKHTSSLKVANEPVLAFTQGSPERDALQKALKDLKGRMEAIPCVVGD
  EEVWTSDVQYQVSPFNHGHKVAKFCYADKSLLNKAIEAALAARKEWDLKPIADRAQIFLKAADMLSGPRR
  AEILAKTMVGQGKTVIQAEIDAAAELIDFFRFNAKYAVELEGQQPISVPPSTNSTVYRGLEGFVAAISPF
  NFTAIGGNLAGAPALMGNVVLWKPSDTAMLASYAVYRILREAGLPPNIIQFVPADGPLFGDTVTSSEHLC
  GINFTGSVPTFKHLWKQVAQNLDRFHTFPRLAGECGGKNFHFVHRSADVESVVSGTLRSAFEYGGQKCSA
  CSRLYVPHSLWPQIKGRLLEEHSRIKVGDPAEDFGTFFSAVIDAKSFARIKKWLEHARSSPSLTILAGGK
  CDDSVGYFVEPCIVESKDPQEPIMKEEIFGPVLSVYVYPDDKYKETLQLVDSTTSYGLTGAVFSQDKDVV
  QEATKVLRNAAGNFYINDKSTGSIVGQQPFGGARASGTNDKPGGPHYILRWTSPQVIKETHKPLGDWSYA
  YMQ
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_170726
• ORF Size: 1689 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_170726.3
• RefSeq Size: 2147 bp
• RefSeq ORF: 1692 bp
• Locus ID: 8659
• UniProt ID: P30038
• Cytogenetics: 1p36.13
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Metabolic pathways
• Gene Summary: This protein belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2009]