BAAT1 (BRAT1) (NM_152743) Human Tagged ORF Clone

CAT#: RG204822

BRAT1 (tGFP-tagged) - Human BRCA1-associated ATM activator 1 (BRAT1)

Product Images

Circular map for RG204822

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: BAAT1
• Synonyms: BAAT1; C7orf27; NEDCAS; RMFSL
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204822 representing NM_152743
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCCAGAATGCGCCCAGCTGCTCCCGGCTCTCTGTGCTGTTCTGGTAGATCCCGGGCAGCCGGTGG
  CAGATGACACCTGTTTGGAGAAGCTCCTGGACTGGTTTAAAACGGTCACTGAAGGAGAGTCCAGTGTCGT
  GCTGCTGCAGGAGCACCCCTGCCTGGTGGAGCTGCTGTCCCATGTGCTGAAAGTCCAGGACCTGAGTTCT
  GGGGTCCTCTCCTTCTCACTGCGCCTGGCAGGAACCTTCGCAGCCCAGGAAAACTGCTTCCAGTATCTTC
  AGCAGGGGGAGTTACTACCAGGGCTCTTTGGGGAGCCAGGACCCCTCGGCCGAGCAACCTGGGCCGTCCC
  CACCGTGCGCAGCGGCTGGATCCAGGGCCTGCGCTCCCTGGCACAGCACCCCAGCGCCCTGCGCTTCCTG
  GCCGACCATGGTGCGGTCGACACCATCTTCTCCCTGCAGGGAGACTCCAGCCTGTTTGTGGCCTCGGCGG
  CCAGTCAGCTCCTGGTGCACGTCCTGGCTTTGTCCATGCGAGGTGGAGCCGAGGGGCAGCCCTGCCTGCC
  GGGGGGTGACTGGCCCGCGTGTGCCCAGAAGATCATGGATCACGTTGAAGAGTCCTTGTGCTCCGCGGCC
  ACCCCCAAGGTCACTCAGGCCCTGAACGTCCTGACCACGACCTTCGGGCGCTGCCAGAGCCCCTGGACGG
  AAGCCCTGTGGGTGCGGCTGAGTCCCCGCGTGGCCTGTCTGCTGGAGAGAGACCCCATCCCCGCCGCACA
  CTCGTTCGTGGACCTGCTTCTCTGTGTGGCTCGTTCTCCCGTGTTCAGTTCTTCCGACGGCAGCCTGTGG
  GAGACAGTGGCGCGGGCTCTGAGCTGCCTGGGTCCCACCCACATGGGACCCCTGGCTTTGGGGATCCTGA
  AGCTCGAGCACTGTCCACAGGCACTGAGGACCCAGGCCTTCCAGGTCCTTCTCCAGCCCCTGGCCTGTGT
  CCTGAAGGCCACGGTTCAGGCCCCCGGACCCCCAGGCTTGCTGGACGGGACGGCAGACGATGCCACGACG
  GTGGACACACTCCTGGCCTCCAAGTCGTCCTGCGCCGGCCTCCTGTGCCGCACCCTGGCTCACCTGGAGG
  AGCTGCAGCCGCTGCCCCAGCGCCCTTCACCGTGGCCCCAGGCGTCTCTACTGGGGGCTACAGTGACTGT
  CCTGCGGCTCTGTGACGGCTCGGCTGCCCCTGCCTCCAGTGTGGGGGGCCACCTCTGTGGGACCCTGGCG
  GGCTGCGTCCGGGTCCAGCGAGCAGCCCTCGACTTCCTGGGGACGCTGTCACAGGGGACAGGCCCCCAGG
  AGCTGGTGACGCAGGCGCTTGCTGTCCTCCTGGAGTGCCTCGAGAGCCCCGGCTCCAGCCCCACGGTTCT
  GAAGAAGGCCTTCCAGGCCACGCTCAGGTGGCTCCTGAGCTCACCCAAGACCCCCGGCTGCTCTGATCTC
  GGCCCCCTCATCCCGCAGTTCCTCAGAGAGCTGTTCCCTGTGCTGCAGAAACGCCTGTGCCACCCCTGCT
  GGGAGGTGAGGGACTCCGCCCTCGAGTTCCTGACCCAGCTGAGCAGGCACTGGGGAGGACAGGCTGACTT
  CAGATGCGCACTCTTGGCTTCAGAGGTGCCTCAGCTGGCCCTGCAGCTCCTCCAGGACCCTGAGAGTTAT
  GTCCGAGCGAGTGCAGTGACCGCCATGGGGCAGCTGTCCAGCCAGGGCCTGCACGCCCCCACCAGCCCTG
  AGCATGCAGAGGCCCGGCAGAGCCTGTTCCTGGAGCTCCTGCACATCCTCTCCGTAGACTCGGAGGGCTT
  CCCACGGCGGGCGGTCATGCAAGTCTTCACTGAGTGGCTGCGGGACGGCCACGCCGACGCGGCCCAGGAC
  ACGGAGCAGTTCGTGGCCACTGTGCTGCAGGCGGCGAGCCGAGACCTGGACTGGGAGGTCCGCGCCCAGG
  GCCTGGAGCTGGCCCTCGTGTTCCTGGGCCAGACTTTGGGGCCGCCGCGTACCCACTGCCCCTATGCCGT
  GGCCCTACCCGAGGTGGCCCCAGCCCAGCCACTCACCGAGGCACTGAGGGCTCTCTGCCACGTGGGGCTC
  TTTGACTTCGCCTTTTGTGCCTTGTTTGACTGCGACCGCCCTGTGGCGCAGAAGTCTTGTGACCTCCTTC
  TCTTCCTGAGGGACAAGATTGCTTCCTACAGCAGCCTGCGGGAGGCCAGGGGCAGCCCCAACACTGCCTC
  CGCAGAGGCCACCCTGCCGAGGTGGCGGGCGGGTGAGCAGGCCCAGCCCCCAGGGGACCAGGAGCCTGAG
  GCTGTGCTGGCCATGCTCAGGTCCCTAGACCTGGAGGGCCTGCGGAGCACGCTGGCCGAGAGCAGCGACC
  ACGTGGAAAAGAGTCCCCAGTCCCTCCTGCAGGACATGCTGGCCACGGGAGGCTTCCTGCAGGGGGACGA
  GGCCGACTGCTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204822 representing NM_152743
  Red=Cloning site Green=Tags(s)
  
  MDPECAQLLPALCAVLVDPGQPVADDTCLEKLLDWFKTVTEGESSVVLLQEHPCLVELLSHVLKVQDLSS
  GVLSFSLRLAGTFAAQENCFQYLQQGELLPGLFGEPGPLGRATWAVPTVRSGWIQGLRSLAQHPSALRFL
  ADHGAVDTIFSLQGDSSLFVASAASQLLVHVLALSMRGGAEGQPCLPGGDWPACAQKIMDHVEESLCSAA
  TPKVTQALNVLTTTFGRCQSPWTEALWVRLSPRVACLLERDPIPAAHSFVDLLLCVARSPVFSSSDGSLW
  ETVARALSCLGPTHMGPLALGILKLEHCPQALRTQAFQVLLQPLACVLKATVQAPGPPGLLDGTADDATT
  VDTLLASKSSCAGLLCRTLAHLEELQPLPQRPSPWPQASLLGATVTVLRLCDGSAAPASSVGGHLCGTLA
  GCVRVQRAALDFLGTLSQGTGPQELVTQALAVLLECLESPGSSPTVLKKAFQATLRWLLSSPKTPGCSDL
  GPLIPQFLRELFPVLQKRLCHPCWEVRDSALEFLTQLSRHWGGQADFRCALLASEVPQLALQLLQDPESY
  VRASAVTAMGQLSSQGLHAPTSPEHAEARQSLFLELLHILSVDSEGFPRRAVMQVFTEWLRDGHADAAQD
  TEQFVATVLQAASRDLDWEVRAQGLELALVFLGQTLGPPRTHCPYAVALPEVAPAQPLTEALRALCHVGL
  FDFAFCALFDCDRPVAQKSCDLLLFLRDKIASYSSLREARGSPNTASAEATLPRWRAGEQAQPPGDQEPE
  AVLAMLRSLDLEGLRSTLAESSDHVEKSPQSLLQDMLATGGFLQGDEADCY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152743
• ORF Size: 2463 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152743.2, NP_689956.1
• RefSeq Size: 2713 bp
• RefSeq ORF: 2466 bp
• Locus ID: 221927
• UniProt ID: Q6PJG6
• Cytogenetics: 7p22.3
• Gene Summary: The protein encoded by this ubiquitously expressed gene interacts with the tumor suppressing BRCA1 (breast cancer 1) protein and and the ATM (ataxia telangiectasia mutated) protein. ATM is thought to be a master controller of cell cycle checkpoint signalling pathways that are required for cellular responses to DNA damage such as double-strand breaks that are induced by ionizing radiation and complexes with BRCA1 in the multi-protein complex BASC (BRAC1-associated genome surveillance complex). The protein encoded by this gene is thought to play a role in the DNA damage pathway regulated by BRCA1 and ATM. [provided by RefSeq, Mar 2012]