coproporphyrinogen oxidase (CPOX) (NM_000097) Human Tagged ORF Clone

CAT#: RG204033

CPOX (tGFP-tagged) - Human coproporphyrinogen oxidase (CPOX)

Product Images

Circular map for RG204033

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: coproporphyrinogen oxidase
• Synonyms: COX; CPO; CPX; HARPO; HCP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204033 representing NM_000097
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTTGCAGCTGGGCAGGCTGAGCTCGGGCCCCTGCTGGCTCGTGGCGCGGGGCGGCTGCGGAGGGC
  CCCGCGCCTGGTCCCAGTGCGGCGGCGGAGGGCTCCGAGCCTGGTCCCAGCGCAGCGCAGCCGGACGCGT
  CTGCCGGCCCCCTGGCCCGGCTGGCACGGAGCAGAGCCGCGGGCTGGGGCACGGCTCGACGTCGAGAGGC
  GGCCCCTGGGTGGGGACAGGGCTGGCCGCGGCGCTGGCGGGGTTGGTGGGGCTGGCCACCGCCGCCTTCG
  GGCATGTGCAGCGGGCGGAGATGTTGCCTAAGACCTCGGGGACGCGGGCCACTTCGCTGGGGAGGCCGGA
  GGAGGAGGAGGATGAGCTGGCCCACCGCTGCAGCAGCTTCATGGCCCCGCCTGTGACCGACCTGGGCGAG
  CTGCGAAGGAGGCCGGGCGACATGAAGACCAAGATGGAGCTGCTGATTCTGGAGACCCAGGCCCAGGTGT
  GCCAGGCTCTGGCACAGGTAGACGGGGGCGCCAACTTTTCTGTGGACCGGTGGGAGAGGAAGGAAGGAGG
  TGGCGGCATCAGCTGTGTACTTCAAGATGGGTGTGTTTTCGAAAAGGCTGGGGTGAGCATTTCTGTTGTT
  CATGGAAATCTTTCAGAGGAAGCTGCAAAACAAATGAGAAGCAGAGGAAAAGTTCTGAAGACTAAAGATG
  GTAAATTGCCATTTTGTGCTATGGGCGTGAGCTCTGTTATCCACCCCAAGAATCCTCATGCTCCTACTAT
  CCATTTCAACTACAGATACTTTGAAGTAGAAGAAGCTGATGGCAACAAGCAGTGGTGGTTTGGTGGTGGA
  TGTGACCTCACTCCAACATACTTGAATCAAGAAGACGCTGTCCATTTTCACAGAACTCTGAAGGAGGCTT
  GTGACCAGCATGGTCCAGATCTCTACCCCAAATTTAAAAAATGGTGTGATGATTACTTCTTTATAGCCCA
  TCGTGGAGAGCGGCGGGGCATTGGTGGTATCTTTTTTGATGATCTTGACTCTCCGTCCAAGGAGGAGGTG
  TTTCGCTTTGTACAGAGCTGTGCCAGGGCTGTAGTTCCTTCTTACATTCCCCTTGTGAAAAAGCACTGTG
  ATGACTCATTCACCCCCCAGGAGAAGCTGTGGCAGCAGCTCAGAAGAGGACGGTATGTAGAATTTAATCT
  GCTGTATGATCGGGGCACAAAGTTTGGCCTCTTCACTCCAGGATCCAGAATTGAAAGTATCTTGATGTCT
  TTACCTCTAACTGCCCGATGGGAGTACATGCATTCACCCTCAGAGAATTCCAAAGAAGCTGAAATTCTGG
  AAGTTCTACGCCATCCAAGGGACTGGGTGCGT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204033 representing NM_000097
  Red=Cloning site Green=Tags(s)
  
  MALQLGRLSSGPCWLVARGGCGGPRAWSQCGGGGLRAWSQRSAAGRVCRPPGPAGTEQSRGLGHGSTSRG
  GPWVGTGLAAALAGLVGLATAAFGHVQRAEMLPKTSGTRATSLGRPEEEEDELAHRCSSFMAPPVTDLGE
  LRRRPGDMKTKMELLILETQAQVCQALAQVDGGANFSVDRWERKEGGGGISCVLQDGCVFEKAGVSISVV
  HGNLSEEAAKQMRSRGKVLKTKDGKLPFCAMGVSSVIHPKNPHAPTIHFNYRYFEVEEADGNKQWWFGGG
  CDLTPTYLNQEDAVHFHRTLKEACDQHGPDLYPKFKKWCDDYFFIAHRGERRGIGGIFFDDLDSPSKEEV
  FRFVQSCARAVVPSYIPLVKKHCDDSFTPQEKLWQQLRRGRYVEFNLLYDRGTKFGLFTPGSRIESILMS
  LPLTARWEYMHSPSENSKEAEILEVLRHPRDWVR
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000097
• ORF Size: 1362 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000097.4, NP_000088.3
• RefSeq Size: 2728 bp
• RefSeq ORF: 1365 bp
• Locus ID: 1371
• UniProt ID: P36551
• Cytogenetics: 3q11.2
• Domains: Coprogen_oxidas
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Porphyrin and chlorophyll metabolism
• Gene Summary: The protein encoded by this gene is the sixth enzyme of the heme biosynthetic pathway. The encoded enzyme is soluble and found in the intermembrane space of mitochondria. This enzyme catalyzes the stepwise oxidative decarboxylation of coproporphyrinogen III to protoporphyrinogen IX, a precursor of heme. Defects in this gene are a cause of hereditary coproporphyria (HCP).[provided by RefSeq, Oct 2009]