CPA3 (NM_001870) Human Tagged ORF Clone

CAT#: RG203145

CPA3 (tGFP-tagged) - Human carboxypeptidase A3 (mast cell) (CPA3)

Product Images

Circular map for RG203145

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CPA3
• Synonyms: MC-CPA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203145 representing NM_001870
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCTCATCCTGCCTGTGGGTTTGATTGCTACCACTCTTGCAATTGCTCCTGTCCGCTTTGACAGGG
  AGAAGGTGTTCCGCGTGAAGCCCCAGGATGAAAAACAAGCAGACATCATAAAGGACTTGGCCAAAACCAA
  TGAGCTTGACTTCTGGTATCCAGGTGCCACCCACCACGTAGCTGCTAAAATGATGGTGGATTTCCGAGTT
  AGTGAGAAGGAATCCCAAGCCATCCAGTCTGCCTTGGATCAAAATAAAATGCACTATGAAATCTTGATTC
  ATGATCTACAAGAAGAGATTGAGAAACAGTTTGATGTTAAAGAAGATATCCCAGGCAGGCACAGCTACGC
  AAAATACAATAATTGGGAAAAGATTGTGGCTTGGACTGAAAAGATGATGGATAAGTATCCTGAAATGGTC
  TCTCGTATTAAAATTGGATCTACTGTTGAAGATAATCCACTATATGTTCTGAAGATTGGGGAAAAGAATG
  AAAGAAGAAAGGCTATTTTTATGGATTGTGGCATTCACGCACGAGAATGGGTCTCCCCAGCATTCTGCCA
  GTGGTTTGTCTATCAGGCAACCAAAACTTATGGGAGAAACAAAATTATGACCAAACTCTTGGACCGAATG
  AATTTTTACATTCTTCCTGTGTTCAATGTTGATGGATATATTTGGTCATGGACAAAGAACCGCATGTGGA
  GAAAAAATCGTTCCAAGAACCAAAACTCCAAATGCATCGGCACTGACCTCAACAGGAATTTTAATGCTTC
  ATGGAACTCCATTCCTAACACCAATGACCCATGTGCAGATAACTATCGGGGCTCTGCACCAGAGTCCGAG
  AAAGAGACGAAAGCTGTCACTAATTTCATTAGAAGCCACCTGAATGAAATCAAGGTTTACATCACCTTCC
  ATTCCTACTCCCAGATGCTATTGTTTCCCTATGGATATACATCAAAACTGCCACCTAACCATGAGGACTT
  GGCCAAAGTTGCAAAGATTGGCACTGATGTTCTATCAACTCGATATGAAACCCGCTACATCTATGGCCCA
  ATAGAATCAACAATTTACCCGATATCAGGTTCTTCTTTAGACTGGGCTTATGACCTGGGCATCAAACACA
  CATTTGCCTTTGAGCTCCGAGATAAAGGCAAATTTGGTTTTCTCCTTCCAGAATCCCGGATAAAGCCAAC
  GTGCAGAGAGACCATGCTAGCTGTCAAATTTATTGCCAAGTATATCCTCAAGCATACTTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203145 representing NM_001870
  Red=Cloning site Green=Tags(s)
  
  MRLILPVGLIATTLAIAPVRFDREKVFRVKPQDEKQADIIKDLAKTNELDFWYPGATHHVAAKMMVDFRV
  SEKESQAIQSALDQNKMHYEILIHDLQEEIEKQFDVKEDIPGRHSYAKYNNWEKIVAWTEKMMDKYPEMV
  SRIKIGSTVEDNPLYVLKIGEKNERRKAIFMDCGIHAREWVSPAFCQWFVYQATKTYGRNKIMTKLLDRM
  NFYILPVFNVDGYIWSWTKNRMWRKNRSKNQNSKCIGTDLNRNFNASWNSIPNTNDPCADNYRGSAPESE
  KETKAVTNFIRSHLNEIKVYITFHSYSQMLLFPYGYTSKLPPNHEDLAKVAKIGTDVLSTRYETRYIYGP
  IESTIYPISGSSLDWAYDLGIKHTFAFELRDKGKFGFLLPESRIKPTCRETMLAVKFIAKYILKHTS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001870
• ORF Size: 1251 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001870.1, NP_001861.1
• RefSeq Size: 1633 bp
• RefSeq ORF: 1254 bp
• Locus ID: 1359
• UniProt ID: P15088
• Cytogenetics: 3q24
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Renin-angiotensin system
• Gene Summary: This gene encodes a member of the carboxypeptidase A family of zinc metalloproteases. The encoded preproprotein is proteolytically processed to generate a mature protease that is released by mast cells and may be involved in the degradation of endogenous proteins and the inactivation of venom-associated peptides. Expression of this gene may be elevated in human asthma patients. [provided by RefSeq, Nov 2015]