ABCB7 (NM_004299) Human Tagged ORF Clone

CAT#: RG202944

ABCB7 (tGFP-tagged) - Human ATP-binding cassette, sub-family B (MDR/TAP), member 7 (ABCB7), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RG202944

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ABCB7
• Synonyms: ABC7; ASAT; Atm1p; EST140535
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202944 representing NM_004299
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTGCTCGCGATGCATTCTTGGCGCTGGGCGGCCGCGGCGGCTGCTTTCGAAAAGCGCCGGCACT
  CCGCGATTCTGATCCGGCCTTTAGTCTCTGTTAGCGGCTCAGGTCCGCAGTGGAGGCCACATCAACTCGG
  CGCCTTGGGAACCGCTCGAGCCTACCAGCAGATTCCAGAGTCATTAAAAAGTATCACATGGCAGAGATTG
  GGAAAAGGCAATTCAGGACAGTTCTTAGATGCTGCAAAGGCTCTCCAGGTATGGCCACTGATAGAAAAGA
  GGACATGTTGGCATGGTCATGCAGGAGGAGGACTCCACACAGACCCAAAAGAAGGGTTAAAAGATGTTGA
  TACTCGGAAAATCATAAAAGCAATGCTTTCTTATGTGTGGCCCAAAGACAGGCCAGATCTACGAGCTAGA
  GTTGCCATTTCGCTGGGATTTTTGGGTGGTGCAAAGGCCATGAATATTGTGGTTCCCTTCATGTTTAAAT
  ATGCTGTAGACAGCCTCAACCAGATGTCGGGAAACATGCTGAACCTGAGTGATGCACCAAATACAGTTGC
  AACCATGGCAACAGCAGTTCTGATTGGCTATGGTGTATCAAGAGCTGGAGCTGCTTTTTTTAACGAAGTT
  CGAAATGCAGTATTTGGCAAGGTAGCCCAGAATTCAATCCGAAGAATAGCCAAAAATGTCTTTCTCCATC
  TTCACAACCTGGATCTGGGTTTTCACCTGAGCAGACAGACGGGAGCTTTATCTAAGGCTATTGACAGAGG
  AACAAGGGGTATCAGTTTTGTCCTGAGTGCTTTGGTATTTAATCTTCTTCCCATCATGTTTGAAGTGATG
  CTTGTCAGTGGTGTTTTGTATTACAAATGCGGTGCCCAGTTTGCTTTGGTAACCCTTGGAACACTTGGTA
  CATACACAGCATTCACAGTTGCAGTCACACGGTGGAGAACTAGATTTAGAATAGAAATGAACAAAGCAGA
  TAATGATGCAGGTAATGCTGCTATAGACTCACTGCTGAATTATGAAACTGTGAAGTATTTTAATAATGAA
  AGATATGAAGCACAGAGATATGATGGATTTTTGAAGACGTATGAGACTGCTTCATTGAAAAGTACCTCTA
  CTCTGGCTATGCTGAACTTTGGTCAAAGTGCTATTTTCAGTGTCGGTTTAACAGCTATAATGGTGCTCGC
  CAGTCAGGGAATTGTGGCAGGTACCCTTACTGTTGGAGATCTAGTAATGGTGAATGGACTGCTTTTTCAG
  CTTTCATTACCCCTGAACTTTCTGGGAACTGTATATAGAGAGACTAGACAAGCACTCATAGATATGAACA
  CCTTGTTTACTCTACTCAAGGTAGACACCCAAATTAAAGACAAAGTGATGGCATCTCCCCTTCAGATCAC
  ACCACAGACAGCTACCGTGGCCTTTGATAATGTGCATTTTGAATACATTGAGGGCCAGAAAGTCCTTAGT
  GGAATATCCTTTGAAGTCCCTGCAGGAAAGAAAGTGGCCATTGTAGGAGGTAGTGGGTCAGGGAAAAGCA
  CAATAGTGAGGCTATTATTTCGCTTCTATGAGCCTCAAAAGGGTAGCATTTATCTTGCTGGTCAAAATAT
  ACAAGATGTGAGCCTGGAAAGCCTTCGGAGGGCAGTGGGAGTGGTACCTCAGGATGCTGTCCTCTTCCAT
  AATACTATTTATTACAACCTCTTATATGGAAACATCAGTGCTTCACCTGAGGAAGTGTATGCAGTGGCAA
  AATTAGCTGGACTTCATGATGCAATTCTTCGAATGCCACATGGATATGACACCCAAGTAGGGGAACGAGG
  ACTCAAGCTTTCAGGAGGAGAAAAGCAAAGAGTAGCAATTGCAAGAGCCATTTTGAAGGACCCCCCAGTC
  ATACTCTATGATGAAGCTACTTCATCGTTAGATTCGATTACTGAAGAGACTATTCTTGGTGCCATGAAGG
  ATGTGGTCAAACACAGAACTTCTATTTTCATTGCACACAGATTGTCAACAGTGGTTGATGCAGATGAAAT
  CATTGTCTTGGATCAGGGTAAGGTAGCCGAACGTGGTACCCACCATGGTTTGCTTGCTAACCCTCATAGT
  ATCTATTCAGAAATGTGGCATACACAGAGCAGCCGTGTGCAGAACCATGATAACCCCAAATGGGAAGCAA
  AGAAAGAAAATATATCCAAAGAGGAGGAAAGAAAGAAACTACAAGAAGAAATTGTCAATAGTGTGAAAGG
  CTGTGGAAACTGTTCGTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202944 representing NM_004299
  Red=Cloning site Green=Tags(s)
  
  MALLAMHSWRWAAAAAAFEKRRHSAILIRPLVSVSGSGPQWRPHQLGALGTARAYQQIPESLKSITWQRL
  GKGNSGQFLDAAKALQVWPLIEKRTCWHGHAGGGLHTDPKEGLKDVDTRKIIKAMLSYVWPKDRPDLRAR
  VAISLGFLGGAKAMNIVVPFMFKYAVDSLNQMSGNMLNLSDAPNTVATMATAVLIGYGVSRAGAAFFNEV
  RNAVFGKVAQNSIRRIAKNVFLHLHNLDLGFHLSRQTGALSKAIDRGTRGISFVLSALVFNLLPIMFEVM
  LVSGVLYYKCGAQFALVTLGTLGTYTAFTVAVTRWRTRFRIEMNKADNDAGNAAIDSLLNYETVKYFNNE
  RYEAQRYDGFLKTYETASLKSTSTLAMLNFGQSAIFSVGLTAIMVLASQGIVAGTLTVGDLVMVNGLLFQ
  LSLPLNFLGTVYRETRQALIDMNTLFTLLKVDTQIKDKVMASPLQITPQTATVAFDNVHFEYIEGQKVLS
  GISFEVPAGKKVAIVGGSGSGKSTIVRLLFRFYEPQKGSIYLAGQNIQDVSLESLRRAVGVVPQDAVLFH
  NTIYYNLLYGNISASPEEVYAVAKLAGLHDAILRMPHGYDTQVGERGLKLSGGEKQRVAIARAILKDPPV
  ILYDEATSSLDSITEETILGAMKDVVKHRTSIFIAHRLSTVVDADEIIVLDQGKVAERGTHHGLLANPHS
  IYSEMWHTQSSRVQNHDNPKWEAKKENISKEEERKKLQEEIVNSVKGCGNCSC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004299
• ORF Size: 2259 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004299.6
• RefSeq Size: 2404 bp
• RefSeq ORF: 2262 bp
• Locus ID: 22
• UniProt ID: O75027
• Cytogenetics: Xq13.3
• Domains: ABC_membrane, ABC_tran, AAA
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters
• Gene Summary: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a half-transporter involved in the transport of heme from the mitochondria to the cytosol. With iron/sulfur cluster precursors as its substrates, this protein may play a role in metal homeostasis. Mutations in this gene have been associated with mitochondrial iron accumulation and isodicentric (X)(q13) and sideroblastic anemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012]