DPM1 (NM_003859) Human Tagged ORF Clone

CAT#: RG202817

DPM1 (tGFP-tagged) - Human dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit (DPM1)

Product Images

Circular map for RG202817

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DPM1
• Synonyms: CDGIE; MPDS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202817 representing NM_003859
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCCTTGGAAGTCAGTCGTAGTCCTCGCAGGTCTCGGCGGGAGCTGGAAGTGCGCAGTCCACGAC
  AGAACAAATATTCGGTGCTTTTACCTACCTACAACGAGCGCGAGAACCTGCCGCTCATCGTGTGGCTGCT
  GGTGAAAAGCTTCTCCGAGAGTGGAATCAACTATGAAATTATAATCATAGATGATGGAAGCCCAGATGGA
  ACAAGGGATGTTGCTGAACAGTTGGAGAAGATCTATGGGTCAGACAGAATTCTTCTAAGACCACGAGAGA
  AAAAGTTGGGACTAGGAACTGCATATATTCATGGAATGAAACATGCCACAGGAAACTACATCATTATTAT
  GGATGCTGATCTCTCACACCATCCAAAATTTATTCCTGAATTTATTAGGAAGCAAAAGGAGGGTAATTTT
  GATATTGTCTCTGGAACTCGCTACAAAGGAAATGGAGGTGTATATGGCTGGGATTTGAAAAGAAAAATAA
  TCAGCCGTGGGGCCAATTTTTTAACTCAGATCTTGCTGAGACCAGGAGCATCTGATTTAACAGGAAGTTT
  CAGATTATACCGAAAAGAAGTTCTAGAGAAATTAATAGAAAAATGTGTTTCTAAAGGCTACGTCTTCCAG
  ATGGAGATGATTGTTCGGGCAAGACAGTTGAATTATACTATTGGCGAGGTTCCAATATCATTTGTGGATC
  GTGTTTATGGTGAATCCAAGTTGGGAGGAAATGAAATAGTATCTTTCTTGAAAGGATTATTGACTCTTTT
  TGCTACTACA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202817 representing NM_003859
  Red=Cloning site Green=Tags(s)
  
  MASLEVSRSPRRSRRELEVRSPRQNKYSVLLPTYNERENLPLIVWLLVKSFSESGINYEIIIIDDGSPDG
  TRDVAEQLEKIYGSDRILLRPREKKLGLGTAYIHGMKHATGNYIIIMDADLSHHPKFIPEFIRKQKEGNF
  DIVSGTRYKGNGGVYGWDLKRKIISRGANFLTQILLRPGASDLTGSFRLYRKEVLEKLIEKCVSKGYVFQ
  MEMIVRARQLNYTIGEVPISFVDRVYGESKLGGNEIVSFLKGLLTLFATT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003859
• ORF Size: 780 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003859.3
• RefSeq Size: 1047 bp
• RefSeq ORF: 783 bp
• Locus ID: 8813
• UniProt ID: O60762
• Cytogenetics: 20q13.13
• Domains: Glycos_transf_2
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2. Mutations in this gene are associated with congenital disorder of glycosylation type Ie. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2015]