C16orf70 (NM_025187) Human Tagged ORF Clone

CAT#: RG202484

C16orf70 (tGFP-tagged) - Human chromosome 16 open reading frame 70 (C16orf70)

Product Images

Circular map for RG202484

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: C16orf70
• Synonyms: C16orf6; lin-10; LIN10
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202484 representing NM_025187
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGACCTGGAGGTAGTGCCCGAACGCTCTCTGGGGAACGAGCAATGGGAATTCACGCTGGGAATGC
  CTCTGGCTCAGGCAGTAGCCATTCTTCAGAAGCACTGTCGCATCATCAAAAACGTCCAGGTTCTCTACAG
  TGAACAGTCTCCTCTAAGCCATGACCTCATTCTTAACCTGACTCAGGACGGGATCAAACTAATGTTTGAT
  GCTTTCAATCAGAGACTTAAGGTGATCGAAGTATGTGATTTGACTAAAGTAAAGTTAAAATATTGTGGCG
  TGCATTTTAATTCTCAGGCCATAGCTCCTACCATTGAACAGATTGACCAGTCTTTTGGCGCAACCCATCC
  TGGAGTGTACAACTCCGCTGAGCAGCTCTTCCATCTCAACTTCAGAGGACTGTCTTTCTCTTTTCAGTTA
  GACTCATGGACTGAGGCTCCAAAGTATGAGCCCAATTTTGCCCATGGCCTGGCTTCTCTCCAGATACCCC
  ATGGAGCAACTGTAAAACGAATGTACATCTACAGTGGCAACAGCCTGCAGGATACCAAGGCTCCCATGAT
  GCCTCTGAGCTGTTTCCTGGGCAATGTCTATGCTGAGAGTGTAGATGTTCTTCGAGATGGAACTGGACCT
  GCAGGTTTACGACTTCGCCTACTTGCTGCAGGTTGTGGACCTGGCCTATTAGCAGATGCCAAGATGCGGG
  TATTTGAACGTTCAGTGTATTTTGGTGATTCCTGCCAAGATGTTCTTAGCATGCTTGGCTCTCCACACAA
  AGTCTTCTATAAATCAGAAGACAAGATGAAAATTCATTCTCCTTCCCCTCATAAACAAGTTCCATCGAAG
  TGTAATGACTACTTTTTTAACTACTTCACTCTTGGAGTGGACATCCTCTTTGATGCGAATACACACAAAG
  TGAAGAAGTTTGTTCTACACACCAATTACCCTGGGCATTATAATTTCAACATTTATCATCGCTGTGAGTT
  CAAGATCCCACTAGCCATAAAGAAAGAAAACGCAGATGGTCAGACAGAAACATGCACGACCTACAGCAAG
  TGGGACAACATCCAGGAGCTCCTGGGCCACCCTGTGGAGAAGCCTGTTGTCCTGCACAGGTCTTCCTCCC
  CAAACAACACCAACCCATTTGGTTCCACATTCTGCTTTGGTCTTCAGCGAATGATCTTTGAGGTCATGCA
  GAACAACCACATTGCCTCGGTGACCCTGTATGGCCCCCCCAGGCCTGGTAGCCACCTGAGAACAGCGGAA
  CTCCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202484 representing NM_025187
  Red=Cloning site Green=Tags(s)
  
  MLDLEVVPERSLGNEQWEFTLGMPLAQAVAILQKHCRIIKNVQVLYSEQSPLSHDLILNLTQDGIKLMFD
  AFNQRLKVIEVCDLTKVKLKYCGVHFNSQAIAPTIEQIDQSFGATHPGVYNSAEQLFHLNFRGLSFSFQL
  DSWTEAPKYEPNFAHGLASLQIPHGATVKRMYIYSGNSLQDTKAPMMPLSCFLGNVYAESVDVLRDGTGP
  AGLRLRLLAAGCGPGLLADAKMRVFERSVYFGDSCQDVLSMLGSPHKVFYKSEDKMKIHSPSPHKQVPSK
  CNDYFFNYFTLGVDILFDANTHKVKKFVLHTNYPGHYNFNIYHRCEFKIPLAIKKENADGQTETCTTYSK
  WDNIQELLGHPVEKPVVLHRSSSPNNTNPFGSTFCFGLQRMIFEVMQNNHIASVTLYGPPRPGSHLRTAE
  LP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025187
• ORF Size: 1266 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025187.5
• RefSeq Size: 2943 bp
• RefSeq ORF: 1269 bp
• Locus ID: 80262
• UniProt ID: Q9BSU1
• Cytogenetics: 16q22.1
• Domains: UPF0183
• Gene Summary: Plays a regulatory role in autophagic activity. In complex with BCAS3, associates with the autophagosome formation site during both non-selective and selective autophagy.[UniProtKB/Swiss-Prot Function]