ALDH16A1 (NM_153329) Human Tagged ORF Clone

CAT#: RG202372

ALDH16A1 (tGFP-tagged) - Human aldehyde dehydrogenase 16 family, member A1 (ALDH16A1), transcript variant 1

Product Images

Circular map for RG202372

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ALDH16A1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202372 representing NM_153329
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGACGCGTGCAGGGCCCCGCGCCCGCGAGATCTTCACCTCGCTGGAGTACGGACCGGTGCCGG
  AGAGCCACGCATGCGCACTGGCCTGGCTGGACACCCAGGACCGGTGCTTGGGCCACTATGTGAATGGGAA
  GTGGTTAAAGCCTGAACACAGAAATTCAGTGCCTTGCCAGGATCCCATCACAGGAGAGAACTTGGCCAGT
  TGCCTGCAGGCACAGGCCGAGGATGTGGCTGCAGCCGTGGAGGCAGCCAGGATGGCATTTAAGGGCTGGA
  GTGCGCACCCCGGCGTCGTCCGGGCCCAGCACCTGACCAGGCTGGCCGAGGTGATCCAGAAGCACCAGCG
  GCTGCTGTGGACCCTGGAATCCCTGGTGACTGGGCGGGCTGTTCGAGAGGTTCGAGACGGGGACGTCCAG
  CTGGCCCAGCAGCTGCTCCACTACCATGCAATCCAGGCATCCACCCAGGAGGAGGCACTGGCAGGCTGGG
  AGCCCATGGGAGTAATTGGCCTCATCCTGCCACCCACATTCTCCTTCCTTGAGATGATGTGGAGGATTTG
  CCCTGCCCTGGCTGTGGGCTGCACCGTGGTGGCCCTCGTGCCCCCGGCCTCCCCGGCGCCCCTCCTCCTG
  GCCCAGCTGGCGGGGGAGCTGGGCCCCTTCCCGGGAATCCTGAATGTCGTCAGTGGCCCTGCGTCCCTGG
  TGCCCATCCTGGCCTCCCAGCCTGGAATCCGGAAGGTGGCCTTCTGCGGAGCCCCGGAGGAAGGGCGTGC
  CCTTCGACGGAGCCTGGCGGGTGAGTGTGCGGAGCTGGGCCTGGCGCTGGGGACGGAGTCGCTGCTGCTG
  CTGACGGACACGGCGGACGTAGACTCGGCCGTGGAGGGTGTCGTGGACGCCGCCTGGTCCGACCGCGGCC
  CGGGTGGCCTCAGGCTCCTCATCCAGGAGTCTGTGTGGGATGAAGCCATGAGACGGCTGCAGGAGCGGAT
  GGGGCGGCTTCGGAGTGGCCGAGGGCTGGATGGGGCCGTGGACATGGGGGCCCGGGGGGCTGCCGCATGT
  GACCTGGTCCAGCGCTTTGTGCGTGAGGCCCAGAGCCAGGGTGCACAGGTGTTCCAGGCTGGTGATGTGC
  CTTCGGAACGCCCATTCTATCCCCCAACCTTGGTCTCCAACCTGCCCCCAGCCTCCCCATGTGCCCAGGT
  GGAGGTGCCGTGGCCTGTGGTCGTGGCCTCCCCCTTCCGCACAGCCAAGGAGGCACTGTTGGTGGCCAAC
  GGGACGCCCCGCGGGGGCAGCGCCAGTGTGTGGAGCGAGAGGCTGGGGCAGGCGCTGGAGCTGGGCTATG
  GGCTCCAAGTGGGCACTGTCTGGATCAACGCCCACGGCCTCAGAGACCCTTCGGTGCCCACAGGCGGCTG
  CAAGGAGAGTGGGTGTTCCTGGCACGGGGGCCCAGACGGGCTGTATGAGTATCTGCGGCCCTCAGGGACC
  CCTGCCCGGCTGTCCTGCCTCTCCAAGAACCTGAACTATGACACCTTTGGCCTCGCTGTTCCCTCAACCC
  TGCCGGCTGGGCCTGAAATAGGGCCCAGCCCAGCACCCCCCTATGGGCTCTTCGTTGGGGGCCGTTTCCA
  GGCTCCTGGGGCCCGAAGCTCCAGGCCCATCCGGGATTCGTCTGGCAACCTCCATGGCTACGTGGCTGAG
  GGTGGAGCCAAGGACATCCGAGGTGCTGTGGAGGCCGCTCACCAGGCTTTCCCTGGCTGGGCGGGCCAGT
  CCCCAGGAGCCCGGGCAGCCCTGCTGTGGGCCCTGGCGGCTGCACTGGAGCGCCGGAAGTCTACCCTGGC
  CTCGAGGCTGGAGAGGCAGGGAGCGGAGCTCAAGGCTGCGGAGGCGGAGGTGGAGCTGAGCGCAAGACGA
  CTTCGGGCGTGGGGGGCCCGGGTGCAGGCCCAAGGCCACACCCTGCAGGTAGCCGGGCTGAGAGGCCCTG
  TGCTGCGCCTGCGGGAGCCGCTGGGTGTGCTGGCTGTGGTGTGTCCGGACGAGTGGCCCCTGCTTGCCTT
  CGTGTCCCTGCTGGCTCCCGCCCTGGCCTACGGCAACACTGTGGTCATGGTGCCCAGTGCGGCCTGTCCT
  CTGCTGGCCCTGGAGGTCTGCCAGGACATGGCCACCGTGTTCCCAGCAGGCCTGGCCAACGTGGTGACAG
  GAGACCGGGACCATCTGACCCGCTGCCTGGCCTTGCACCAAGACGTCCAGGCCATGTGGTATTTCGGATC
  AGCCCAGGGTTCCCAGTTTGTCGAGTGGGCCTCGGCAGGAAACCTCAAACCGGTGTGGGCGAGCAGGGGC
  TGCCCGCGGGCCTGGGACCAGGAGGCCGAGGGGGCAGGCCCAGAGCTGGGGCTGCGAGTGGCGCGGACCA
  AGGCCCTGTGGCTGCCTATGGGGGAC
  
  ACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202372 representing NM_153329
  Red=Cloning site Green=Tags(s)
  
  MAATRAGPRAREIFTSLEYGPVPESHACALAWLDTQDRCLGHYVNGKWLKPEHRNSVPCQDPITGENLAS
  CLQAQAEDVAAAVEAARMAFKGWSAHPGVVRAQHLTRLAEVIQKHQRLLWTLESLVTGRAVREVRDGDVQ
  LAQQLLHYHAIQASTQEEALAGWEPMGVIGLILPPTFSFLEMMWRICPALAVGCTVVALVPPASPAPLLL
  AQLAGELGPFPGILNVVSGPASLVPILASQPGIRKVAFCGAPEEGRALRRSLAGECAELGLALGTESLLL
  LTDTADVDSAVEGVVDAAWSDRGPGGLRLLIQESVWDEAMRRLQERMGRLRSGRGLDGAVDMGARGAAAC
  DLVQRFVREAQSQGAQVFQAGDVPSERPFYPPTLVSNLPPASPCAQVEVPWPVVVASPFRTAKEALLVAN
  GTPRGGSASVWSERLGQALELGYGLQVGTVWINAHGLRDPSVPTGGCKESGCSWHGGPDGLYEYLRPSGT
  PARLSCLSKNLNYDTFGLAVPSTLPAGPEIGPSPAPPYGLFVGGRFQAPGARSSRPIRDSSGNLHGYVAE
  GGAKDIRGAVEAAHQAFPGWAGQSPGARAALLWALAAALERRKSTLASRLERQGAELKAAEAEVELSARR
  LRAWGARVQAQGHTLQVAGLRGPVLRLREPLGVLAVVCPDEWPLLAFVSLLAPALAYGNTVVMVPSAACP
  LLALEVCQDMATVFPAGLANVVTGDRDHLTRCLALHQDVQAMWYFGSAQGSQFVEWASAGNLKPVWASRG
  CPRAWDQEAEGAGPELGLRVARTKALWLPMGD
  
  TRPLE - GFP Tag - V
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153329
• ORF Size: 2406 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153329.2, NP_699160.1
• RefSeq Size: 2627 bp
• RefSeq ORF: 2409 bp
• Locus ID: 126133
• UniProt ID: Q8IZ83
• Cytogenetics: 19q13.33
• Domains: aldedh
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the aldehyde dehydrogenase superfamily. The family members act on aldehyde substrates and use nicotinamide adenine dinucleotide phosphate (NADP) as a cofactor. This gene is conserved in chimpanzee, dog, cow, mouse, rat, and zebrafish. The protein encoded by this gene interacts with maspardin, a protein that when truncated is responsible for Mast syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]