ACY3 (NM_080658) Human Tagged ORF Clone

CAT#: RG202287

ACY3 (tGFP-tagged) - Human aspartoacylase (aminocyclase) 3 (ACY3)

Product Images

Circular map for RG202287

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ACY3
• Synonyms: ACY-3; HCBP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202287 representing NM_080658
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCTCACTGCCTGTGCCCCGGGAGCCCCTGCGTCGCGTGGCTGTGACTGGGGGCACGCATGGCAACG
  AGATGTCGGGCGTCTACCTGGCCCGGCACTGGCTGCATGCCCCCGCAGAGCTGCAGAGAGCCAGCTTCTC
  CGCTGTGCCTGTGCTGGCCAACCCGGCAGCCACATCCGGCTGCCGCCGCTACGTGGACCATGACCTCAAC
  CGCACCTTCACCAGCAGCTTCCTCAATTCCAGGCCCACCCCGGACGACCCATATGAGGTGACAAGAGCCC
  GAGAGCTGAACCAGCTGCTGGGGCCCAAGGCCTCGGGCCAGGCCTTTGACTTTGTCCTTGACCTGCACAA
  CACCACGGCCAACATGGGCACCTGCTTAATCGCGAAGTCCTCCCACGAAGTCTTTGCCATGCACCTGTGC
  CGCCATCTGCAGCTGCAGTACCCCGAGCTGTCCTGCCAGGTCTTCCTGTACCAGCGGTCTGGGGAGGAGA
  GCTACAACCTGGACTCTGTGGCCAAAAATGGACTGGGTCTGGAGCTGGGCCCCCAGCCACAGGGTGTGCT
  GCGGGCTGACATTTTCTCAAGGATGAGGACCCTGGTGGCCACAGTTCTGGACTTCATCGAACTCTTCAAC
  CAGGGTACGGCCTTTCCTGCCTTTGAGATGGAAGCCTATAGACCCGTGGGCGTCGTGGACTTCCCCCGCA
  CCGAGGCCGGGCACCTGGCAGGCACTGTGCATCCTCAGCTGCAGGACCGAGACTTCCAGCCACTGCAGCC
  TGGTGCTCCCATCTTCCAGATGTTCAGTGGGGAGGACCTGCTCTATGAGGGAGAGTCCACGGTGTACCCC
  GTGTTCATTAACGAGGCTGCCTACTATGAGAAGGGCGTTGCCTTTGTCCAGACTGAGAAGTTCACATTCA
  CCGTGCCTGCCATGCCCGCGCTGACCCCTGCCCCGAGCCCAGCTTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202287 representing NM_080658
  Red=Cloning site Green=Tags(s)
  
  MCSLPVPREPLRRVAVTGGTHGNEMSGVYLARHWLHAPAELQRASFSAVPVLANPAATSGCRRYVDHDLN
  RTFTSSFLNSRPTPDDPYEVTRARELNQLLGPKASGQAFDFVLDLHNTTANMGTCLIAKSSHEVFAMHLC
  RHLQLQYPELSCQVFLYQRSGEESYNLDSVAKNGLGLELGPQPQGVLRADIFSRMRTLVATVLDFIELFN
  QGTAFPAFEMEAYRPVGVVDFPRTEAGHLAGTVHPQLQDRDFQPLQPGAPIFQMFSGEDLLYEGESTVYP
  VFINEAAYYEKGVAFVQTEKFTFTVPAMPALTPAPSPAS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080658
• ORF Size: 957 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080658.2
• RefSeq Size: 1317 bp
• RefSeq ORF: 960 bp
• Locus ID: 91703
• UniProt ID: Q96HD9
• Cytogenetics: 11q13.2
• Domains: Aste_AspA
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Histidine metabolism
• Gene Summary: Plays an important role in deacetylating mercapturic acids in kidney proximal tubules. Also acts on N-acetyl-aromatic amino acids (By similarity).[UniProtKB/Swiss-Prot Function]