Calbindin (CALB1) (NM_004929) Human Tagged ORF Clone

CAT#: RG201358

CALB1 (tGFP-tagged) - Human calbindin 1, 28kDa (CALB1)

Product Images

Circular map for RG201358

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Calbindin
• Synonyms: CALB; D-28K
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201358 representing NM_004929
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAATCCCACCTGCAGTCATCCCTCATCACAGCCTCACAGTTTTTCGAGATCTGGCTCCATTTCG
  ACGCTGACGGAAGTGGTTACCTGGAAGGAAAGGAGCTGCAGAACTTGATCCAGGAGCTCCAGCAGGCGCG
  AAAGAAGGCTGGATTGGAGTTATCACCTGAAATGAAAACTTTTGTGGATCAGTATGGGCAAAGAGATGAT
  GGAAAAATAGGAATTGTAGAGTTGGCTCACGTATTACCCACAGAAGAGAATTTCCTGCTGCTCTTCCGAT
  GCCAGCAGCTGAAGTCCTGTGAGGAATTCATGAAGACATGGAGAAAATATGATACTGACCACAGTGGCTT
  CATAGAAACTGAGGAGCTTAAGAACTTTCTAAAGGACCTGCTAGAAAAAGCAAACAAGACTGTTGATGAC
  ACAAAATTAGCCGAGTATACAGACCTAATGCTGAAACTATTTGATTCAAATAATGATGGGAAGCTGGAAT
  TAACTGAGATGGCCAGGTTACTACCAGTGCAGGAGAATTTTCTTCTTAAATTCCAGGGAATCAAAATGTG
  TGGGAAAGAGTTCAATAAGGCTTTTGAGCTGTATGATCAGGACGGCAATGGATACATAGATGAAAATGAA
  CTGGATGCTTTACTGAAGGATCTGTGCGAGAAGAATAAACAGGATCTGGATATTAATAATATTACAACAT
  ACAAGAAGAACATAATGGCTTTGTCGGATGGAGGGAAGCTGTACCGAACGGATCTTGCTCTTATTCTCTG
  TGCTGGGGATAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201358 representing NM_004929
  Red=Cloning site Green=Tags(s)
  
  MAESHLQSSLITASQFFEIWLHFDADGSGYLEGKELQNLIQELQQARKKAGLELSPEMKTFVDQYGQRDD
  GKIGIVELAHVLPTEENFLLLFRCQQLKSCEEFMKTWRKYDTDHSGFIETEELKNFLKDLLEKANKTVDD
  TKLAEYTDLMLKLFDSNNDGKLELTEMARLLPVQENFLLKFQGIKMCGKEFNKAFELYDQDGNGYIDENE
  LDALLKDLCEKNKQDLDINNITTYKKNIMALSDGGKLYRTDLALILCAGDN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004929
• ORF Size: 783 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004929.4
• RefSeq Size: 2531 bp
• RefSeq ORF: 786 bp
• Locus ID: 793
• UniProt ID: P05937
• Cytogenetics: 8q21.3
• Domains: EFh
• Gene Summary: The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease. [provided by RefSeq, Jan 2015]